LOCUS AK056096 2764 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31534 fis, clone NT2RI2000671.
ACCESSION AK056096
VERSION AK056096.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2764)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2764
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2000671"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012710"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 884..1654
/codon_start=1
/protein_id="BAG51623.1"
/translation="MGKTKDIGDDDTVASEFWSGAFSQPSSVPTRPRTPNRDSWRRAW
AARGLHPRPSILQPGPARLSRARAGGTRCPQRRHGRATFCALGRGIGVRRGPGPRPAR
IPGLTLTWKRMSARRMQWAMQTGGRNQTFGGGVPLFWTWLTICCAVWRSLPCRLTHSC
SRAFSSAPLKKTKSSMLPPKQALASAARNLCRGAGCNRQAVAGQLLPSTWSLHAHGLA
KEAPILPVKKISRSCSVNNKVSEKTTKPPTLRSFLSPI"
BASE COUNT 736 a 711 c 697 g 620 t
ORIGIN
1 gctatcattt taggcttaac taccaccatc tcctcgcggg cagtgggggc aaccgcgaag
61 acagaatttc acaaactttc agctccagca gtttcaagga aatgtcaaat gacaagatgg
121 aagaccgagt gtcaagagct aagacaacaa aaaggccccc aaaatttgtt ttcaagaaaa
181 ccaatgaaaa aagcaagcaa tcagaacgtg aagtccagga tcatggatgt gcttatcttc
241 acagtcactg ccgggtcctg aagttctcct ctggcccagt gccagctgcg aagccctctg
301 ccaaagggcc agaggaggcc ggtgatatag agctctgatt acccacccgt cagggcaccg
361 ctgaaggtca ctgaatactt actgtgcacc cgaaaaataa atcctgcgag ttcacgcccg
421 cgtagttcgc cccctgattt tgagcgactc ctttcgcatg ggatctacaa aaccgaactg
481 ccttaaagac ctctttcaca cggacgtgaa gtcacagaac tgacaaaatc ccatcctgtc
541 aaagtgcacg ggtctttgaa atctaacaca aaaagccata gaaagattct ctaaacaccc
601 tgtactaaga ggaacacgga cagggcactg cgttctgaag tagaggccag ggcactggcc
661 cttagacacg tctcgctgtc accgggctaa caacattggc aagggcggcg gcagcagcac
721 tgatatttgc agcccccaag ggctctggcg aaaccccctc tattactctg taccctgcct
781 gcttccaaga tgaacctgtt gctgggaaag aacaggctaa attagaaaag ggagtatttt
841 gtcaaagttg aaggtgagtg atagcctgcc cgcctcaaat aggatgggaa aaactaagga
901 tattggggat gatgacactg tcgcctccga attctggtcc ggggctttct cccagccctc
961 aagtgtcccc acgcggccgc ggaccccgaa cagggacagc tggagacggg cttgggcagc
1021 gcgcggcctg cacccgcggc catccatctt gcagcccggc ccggccaggc tatcccgggc
1081 aagggctggg ggaactcgct gcccccagcg ccgccacggc cgggcgacat tctgcgccct
1141 gggaaggggg ataggggtgc ggcgcggccc cggtccccgg cccgcccgga tccccggcct
1201 caccttgacg tggaagcgga tgagcgccag gcggatgcag tgggccatgc agacgggcgg
1261 caggaaccag acctttggcg gcggggtgcc cttgttctgg acatggctga cgatctgctg
1321 tgccgtctgg cgctcgttgc cctgccgctt gacccactcg tgcagccggg ccttctcgag
1381 cgcgccgttg aagaagacga agagctcgat gttgccgccg aagcaggcct tggccagcgc
1441 cgccagaaac ctctgtagag gagcaggatg caaccgacag gctgtggccg gacagctgct
1501 gcccagcaca tggagcctgc acgcgcacgg tttggccaaa gaagccccca tactcccggt
1561 gaaaaagatt tccagaagct gttctgtcaa taacaaagtc tcagagaaaa ccacaaaacc
1621 accaacacta agatcattct tgagtccaat ttgaaaaact agggtcaagt tctgcagagg
1681 cattgaaagg acaagaaacc accctgatac ccatcgtgtg agggaaaatg ctctattcac
1741 cattcctcag cttctgcttc tggtttcaga gttctctcta tattggaggg tgttttaaag
1801 ctagagtggt ctttatccac ttttattaac acatctgaat gtgaaggtca agagaggaaa
1861 gtgatatgtc ctaagtcaga gtagagtcaa caagaaaata agacaaacag cgactgagcc
1921 cctggtgtat actgggcatt ggccagctac tggggatatg gagatgaaga aaacataacc
1981 cttcttcaag gagcccaacc taccaaggta gacagacata tagacaaata gatacttgca
2041 tagaaaaaaa gaggaaaagg ggatcagtgt gacctgtgta actaagtacc ctataaaccc
2101 tcctgcaaca gatcatattg ccctttatag tggggatggt aatcccatct gaattccaca
2161 ggtactttgc agtcatacca cacccatgtg tctgtcggtc ctgactgtac catttataaa
2221 cagcttcact ttcagcagtt ctcagccctc ttaagctagg gtcattgtca gtagggatac
2281 tgcttcataa gcaccagcag aacaccaaag gagaccatat gggtgaaagc aaccagcact
2341 gccctggcgc ttcataggtt cttagagttt ttatctttta ctttcagtct aacacagcac
2401 tgcctgcttt ttgtttttgt tgcttggttt gtttttttct taccgtgttc accaaacttg
2461 tgtccaaata gctttgggct gatgcaaaaa tatctatgtg gaagagaaga gttgttctca
2521 tggagggcct tcagatgagt gctatagact ctctaggcaa ctccaagagg cttctcaagc
2581 agggtgggca gtgagagctg ctatggaatc aatggacaaa ctgacaggga ctgctttgaa
2641 agacagtact cagttgagta tatatattct ctcttaaggg ctaaaagttt ataatcatcc
2701 cttaaacact ctgtgatggg atcttcagga tcatcttttg aagtaaacta tattttacaa
2761 tgtg
//