LOCUS AK056031 1982 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31469 fis, clone NT2NE2001428.
ACCESSION AK056031
VERSION AK056031.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1982)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1982
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2001428"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000012645"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 439..903
/codon_start=1
/protein_id="BAB71077.1"
/translation="MEFHSCCPSWCTMARSRLTSTSASQCVRNWWVLGLTDFKNEAAH
PHELVRYALPALLTKDNGYLSKPIHHSVSRILRRDQRRFPPRPRKIDRSSLARVAMTH
MRIYKRPSHRDFMWLPFLNGSRFSSASLLTRFCSGSTADPWSSSSSSVSSSL"
BASE COUNT 465 a 531 c 429 g 557 t
ORIGIN
1 gttcattact ttcttgtttt tgccaaatta ataggtgaag atggcctatc aaaatcattt
61 tgattttcac ttctttattt cagatggagt ttctctctta ttttccaggc tgcagtgcag
121 tggcgtgatc tcggctcact gcaacctcca cctcccaggt tcaagcgatt ctcctgtctt
181 agcctcacga gtagccggga ttacaggcac atgcccccat tcccggctaa tttttgtatt
241 tttagtagtg acggggtttc atcatattgg tcaggctggt ctcgaactcc tgacctcagg
301 tgatccgcct tggcctccca aagtgttggg attacaggcg tgagccaccc actgtatctc
361 ttgttcggag gaatatctaa tcaagaaaat catttttact gtttttattt tttggtttgt
421 tcgttttttt cctgtgagat ggagtttcac tcttgttgcc catcctggtg tacaatggcg
481 cgatctcggc tcacttcaac ctccgcctcc cagtgcgtcc ggaattggtg ggttcttggt
541 ctcactgact tcaagaatga agctgcgcac cctcacgaac ttgtcagata tgctctccca
601 gcccttctta ctaaggacaa tgggtacttg tccaagccca tccaccattc tgtcagtcgc
661 atcctaagaa gagatcagag aagattccct cctagaccac gcaaaataga ccgatcatct
721 ttggcacgtg tggccatgac ccacatgagg atttataagc gacctagtca ccgggacttc
781 atgtggttac cttttctgaa tggctcccgg ttctcctctg cttccctgtt gactcggttt
841 tgttctggtt caacagcaga cccctggtca tcaagttcat cttctgtttc atcatcactg
901 tagggaacca cttcgtcatt aggctgagaa tcctcgtcaa atgtcgtttc tgagtctctt
961 tctgtactca ttctgctggc aaattggaac tacctgctta aaaggaaaga gaaaccagag
1021 tgaattatga ctcttgccca gtttttggtt gaatactgcc tgcatgctgc tacaatgccc
1081 cttttacaga acattgtcta acatgtaaat attctggaat agtaccccca aattcaactt
1141 gacatgaaaa caagaacaaa accattcagt actgattaaa tgcttgggat aatgtgcaac
1201 aatctaggtg atccgaagag tatttgatga cattgcatta atgaccttaa gttaatagct
1261 gcacccttct ggttaccgct agaactgagc tttcgctcgc cgtccaccac tgctgttttg
1321 ctgccgtcgc agacccgccg ctgacttcca tccctccaat ccagcagggc gcccgctgtg
1381 ctcctgatcc agggaagcac ccgttgccac tcctgatcgc gctaaaggct tgccattgtt
1441 cctgcatggc taagtgcctg gtttcgacct aatcaagctg aacactagtc actgggttcc
1501 acggttctct tccgtgaccc acgacttcta atagagctat aacactcacc gcacggccca
1561 agattccatt ccttcgaatc cgtgaggcca agaaccccag gtcagagaac acgaggcttg
1621 ccacatcttg gaagtggcct gccgccattt tggaagtggc ccaccaccat cttgggagct
1681 ctcggagcaa ggacccctgg catctggata ttaagactgt ctccagccct ttgaggttga
1741 gagaggaagc aagcacggag gaatggattt gacaacccac atgtccgtga ctcaaagcaa
1801 gcaaggcctt gcacaaccaa ctcgccaccc acacaatctg cacagtctca gtgagaaacc
1861 aggagcaggg agctctccgc tgagagtgaa gaaatgtcct caactcaact tctctgcagt
1921 gtgcatgtgt gtgaaacgca gacacacacg ggaggaattt ggctctgtca ttttagtgta
1981 cc
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