LOCUS AK055994 2168 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31432 fis, clone NT2NE2000550.
ACCESSION AK055994
VERSION AK055994.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2168)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2168
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2000550"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000012608"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 574..1605
/codon_start=1
/protein_id="BAB71067.1"
/translation="MGSPGASLGIKKALQSEQATALPASAPAVSQPTAPAPSCLPKAG
QVIPALLREAPFSSVIAPTLLCGFLFLAWVAAEVPEESSRMAGSGARSEEGRRQHAFV
PEPFDGANVVPNLWLHSFEVINDLNHWDHITKLRFLKESLRGEALGVYNRLSPQDQGD
YGTVKEALLKAFGVPGAAPSHLPKEIVFANSMGKGYYLKGKIGKVPVRFLVDSGAQVS
VVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVAN
ASAEEAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLELIEEDPSS
EEGRQELSH"
BASE COUNT 451 a 574 c 633 g 510 t
ORIGIN
1 gcagcagttg agtccatgta agatgattgt gcacgtgtgt gtgtgtgtgt gtgtgtgtgt
61 gtcctcactc acttaaaaaa gaccatttct gtgcattctt tatgctggaa ccctcctctg
121 cctccctctc caggaaatcc cataggatcc catacctgct ttagaatgtg tgtgagacac
181 ttcccagtgc ctggcatgtg gtaggagctc agtacatgta aactgttttc ctttgaccac
241 tcaaagtagt cacttgttgg ggaggccaat ggcggcttcc ccggtctctg agctgatggg
301 cgtctggtcc ctgcctcccc ttccttcact ggctgatgac aaatacctga atgtcatcac
361 atcctccgtg tcctcatctg ttattctgga acacaggcca gggagggtac agggtagtcc
421 cccttcaaca gaggctggcc tttcttggga tgtgggctgg ctggtgttgg tccaaggatg
481 gatgcagagg gtgaggcacc catcctgcta gtccggccgg atgctggcag gagggcgggg
541 tgaggagggg cggagcttcc agaacaaagg agaatgggga gcccaggggc cagcctaggc
601 atcaaaaagg ctctgcagag tgaacaggcc acagcactgc ctgcctctgc cccagcagtc
661 agccagccga ccgcgcctgc tccctcctgc ttgcccaagg ccgggcaagt catccccgct
721 ctgcttcgag aggccccgtt ttccagcgtg attgcgccga cactgctctg tgggtttctc
781 ttcttggcgt gggttgctgc tgaggttcca gaggagagca gcaggatggc cgggagcgga
841 gccaggagtg aggaaggccg ccggcagcat gccttcgtcc cggaaccttt tgatggggcc
901 aatgtcgtcc caaacctctg gctgcacagc tttgaagtca tcaatgacct caaccattgg
961 gaccatatca ccaagctaag gttcctgaaa gagtccctca gaggagaggc cctgggtgtc
1021 tacaataggc tcagtcccca ggaccaggga gactatggga ctgtgaaaga ggccctcctg
1081 aaggcctttg gggtccctgg ggctgccccc agccacctgc ccaaagagat cgtctttgcc
1141 aacagcatgg gtaagggcta ctatctcaag gggaagattg gcaaagtgcc cgtgaggttc
1201 ctggtggact ctggggccca ggtctctgtg gtccacccaa acttgtggga ggaggtcact
1261 gatggcgatc tggacaccct gcagcccttt gagaatgtgg taaaggtggc caatggtgct
1321 gaaatgaaga tcctgggtgt ctgggataca gcggtgtccc taggcaagct gaagctgaag
1381 gcacagttcc tagtggccaa tgcgagtgcc gaggaagcca tcattggcac tgatgtgctc
1441 caggaccaca atgctatcct ggactttgag caccgcacat gcaccctgaa agggaagaag
1501 tttcgccttc tgcctgtggg agggtccctg gaagatgagt ttgacctgga gctcatagag
1561 gaggacccct cctcagaaga agggcggcag gagctatccc actgagaagc caccttttct
1621 ttaacctcct aaatattggt gggaagaccc accgctgtgg ggggggttgc atatcctcat
1681 gggggtcact gggcttggcc agtctgctta tcaactcttg ctcttctctc ccctttgcct
1741 ccctctgcag gggccttaat ctgcccctgg taggggaggc ttccactgaa caggcacagg
1801 tgagggagag caggctggct tagagggaca gggtccccat ggtcatcaag ctgctgttga
1861 tgacaaagac tcaaaggctg gaagagctcc caaggaagct agaaatgctt gtctttgaaa
1921 gaactgtggg accccttcag attccctgag gtatggcttg gtcactctca ggtcctcaaa
1981 gcctgtctta gttgggctgg gtcctagctg cagggtcttt gtgagggtca cagttgctct
2041 gggacacctc cctgaagagc ctttccacct gtacaatcgt attttctttc tgtcatttgc
2101 tttgaagccc attgtgcctt atgccaataa ttcaattgct gcaaacacca ataaagattg
2161 attcatgg
//