LOCUS AK054633 2403 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ30071 fis, clone ASTRO2000095.
ACCESSION AK054633
VERSION AK054633.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2403)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2403
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2000095"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000011247"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 110..1315
/codon_start=1
/protein_id="BAB70779.1"
/translation="MSRDVCIHTWPCTYYLEPKRRWVTGQLSLTSLSLRFMTDSTGEI
LVSFPLSSIVEIKKEASHFIFSSITILEKGHAKHWFSSLRPSRNVVFSIIEHFWRELL
LSQPGAVADASVPRTRGEELTGLMAGSQKRLEDTARVLHHQGQQLDSVMRGLDKMESD
LEVADRLLTELESPAWWPFSSKLWKTPPETKPREDVSMTSCEPFGKEGILIKIPAVIS
HRTESHVKPGRLTVLVSGLEIHDSSSLLMHRFEREDVDDIKVHSPYEISIRQRFIGKP
DMAYRLISAKMPEVIPILEVQFSKKMELLEDALVLRSARTSSPAEKSCSVWHAASGLM
GCTLHREPPAGDQEGTALHLQTSLPALSEADTQELTQGFRPMSLSTQTVLVMLCSESA
GALWRQRLS"
BASE COUNT 548 a 648 c 648 g 559 t
ORIGIN
1 ctgcgcaggc gccgagcggc cgaggcgccg cggtcggctc tgggactcgt ctggcgtccc
61 tcagaggcag aagaggcctg gaccttggcg cacacagacc caggaacaga tgagcaggga
121 tgtctgcatc cacacctggc cgtgcaccta ctacctggag cccaagaggc gatgggttac
181 tggacagctg tccttaacat cgctgtcgct caggttcatg actgacagca ctggagagat
241 tctggtcagc ttccccctct ccagcatagt tgagatcaag aaggaggctt cacattttat
301 cttcagctcc atcaccatcc tggagaaggg ccatgccaag cactggttca gctccctgcg
361 gccaagtcga aatgtggtct tcagcatcat cgagcatttc tggagggagc tgctgctgtc
421 tcagcctgga gccgtggcag acgcatctgt cccaaggacc cggggcgagg agctgacggg
481 actcatggct ggatcccaga aacgcctgga ggacacggcg agggtcctgc accaccaggg
541 ccagcagctg gacagcgtca tgagaggcct ggacaagatg gagtcagacc tggaggtggc
601 ggacagattg ctgacagaac tggaatctcc tgcttggtgg ccctttagct ccaagctttg
661 gaagacacca ccggaaacaa agcccaggga agatgtctcc atgaccagtt gtgaaccctt
721 tgggaaagaa gggatactga taaaaattcc tgctgttatt tcccacagaa cagagtctca
781 cgttaaacca gggaggctca ccgtccttgt gtctgggttg gaaatacatg actccagttc
841 tttgctcatg cacaggtttg aaagagaaga cgtggacgac atcaaggtcc actcacctta
901 cgaaattagc atccgccagc ggtttattgg aaagccagac atggcctatc gtttgatatc
961 tgccaagatg ccagaggtta tccccatttt agaagtgcag ttcagcaaga agatggagct
1021 gttagaagat gcattggtgc tcagaagcgc aagaacctct tcccccgcag agaagagctg
1081 ctcagtctgg catgcagcat ctgggctgat gggctgtacc ctgcaccgtg agccacccgc
1141 aggagaccag gagggcacag cactgcacct gcagacaagc ctgccagccc tttctgaggc
1201 agatacccag gaactaaccc agggcttccg gcctatgtcg ctgtcaaccc agacagtgtt
1261 ggtgatgctt tgctccgagt ctgcaggagc tttgtggaga cagagacttt cctagcaggg
1321 cagcaagcgg aagcttttct tccagaacag cctttgctca gggcatgagg gagccggcca
1381 ggcccacagc gcccagggca tcctcctcac ggatgtgttg gaggaaagtg gctttggtgc
1441 tatcctgggg gaccatggcc ataagctggc agtgtcagcc acgcatcagt tgcgtttctt
1501 tgttcaggtt gtgcctcttg ctgcctgcct gagtcaccca gggctgctcc ccttcctggc
1561 tctgacgccc tcagcgggtc ccacaagccg ctcaggacca cagagtctgg ccatgggtct
1621 ggccaggcat ccactgatgc tctaagctgc agcttctctt aggaacactt ctatcaggtc
1681 tggctcgcag gcatgcccca gggccagcct ggattgtact gtccttcaag cactccttgc
1741 tctatttctt tgaagtttat aaaagtttgt ttcccccaaa acccaaagca cttttaatca
1801 ggtagtttct gtggaggtcg aactggctgg aaggaaggct ggttcctgga tttctttact
1861 catgtagcag ggactcaagg aagtctgatt tgcgcctcgc ctctgactca gagcccatga
1921 aggttcctgt gtgaggccac tggctgcctc ggagctgaca gcatcctgca caatggctag
1981 atggcttgtc accccaggcc gcagaggaac aactgtttct agggtttggc cagacttcac
2041 attctccatt ctttccatcc ctgttatgtg gaatgcacac gatggcatgt aattacttca
2101 gtcccttctc aaaggaaaaa gaatctggac ccgcactcca ggacactagc agtttgaagg
2161 ttcgctcagg aatgttcttt gtctgcagac ctagcagctg tacagatcgc tcttgtgttt
2221 ttgcactatt aattaacttc aagtgcaatt ttggcaggca ggccaagggt ctcaacaggc
2281 tatttggaat gagttctctt ttaaggcctt caacgtagtg agacattttc aaatcaaaaa
2341 gtacagcagg agccagcctt tattttgtct gtgtacacaa taaaatattc cgcttctata
2401 ggc
//