LOCUS AK031621 2375 bp mRNA linear HTC 06-OCT-2010
DEFINITION Mus musculus 13 days embryo male testis cDNA, RIKEN full-length
enriched library, clone:6030463A22 product:LEFT-RIGHT
DETERMINATION FACTOR B PRECURSOR (LEFTY-2 PROTEIN), full insert
sequence.
ACCESSION AK031621
VERSION AK031621.1
KEYWORDS HTC_FLI; HTC; CAP trapper.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 2375)
AUTHORS Adachi,J., Aizawa,K., Akimura,T., Arakawa,T., Bono,H.,
Carninci,P., Fukuda,S., Furuno,M., Hanagaki,T., Hara,A.,
Hashizume,W., Hayashida,K., Hayatsu,N., Hiramoto,K., Hiraoka,T.,
Hirozane,T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I.,
Kasukawa,T., Katoh,H., Kawai,J., Kojima,Y., Kondo,S., Konno,H.,
Kouda,M., Koya,S., Kurihara,C., Matsuyama,T., Miyazaki,A.,
Murata,M., Nakamura,M., Nishi,K., Nomura,K., Numazaki,R., Ohno,M.,
Ohsato,N., Okazaki,Y., Saito,R., Saitoh,H., Sakai,C., Sakai,K.,
Sakazume,N., Sano,H., Sasaki,D., Shibata,K., Shinagawa,A.,
Shiraki,T., Sogabe,Y., Tagami,M., Tagawa,A., Takahashi,F.,
Takaku-Akahira,S., Takeda,Y., Tanaka,T., Tomaru,A., Toya,T.,
Yasunishi,A., Muramatsu,M. and Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (16-JUL-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Yoshihide Hayashizaki
The Institute of Physical and Chemical Research (RIKEN), Omics
Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
URL :http://www.osc.riken.jp/
REFERENCE 2
AUTHORS
CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and
Genome Science Group (Genome Network Project Core Group)
TITLE The Transcriptional Landscape of the Mammalian Genome
JOURNAL Science 309, 1559-1563 (2005)
REFERENCE 3
AUTHORS
CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group
(Genome Network Project Core Group) and the FANTOM Consortium
TITLE Antisense Transcription in the Mammalian Transcriptome
JOURNAL Science 309, 1564-1566 (2005)
REFERENCE 4
AUTHORS
CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research
Group Phase I and II Team
TITLE Analysis of the mouse transcriptome based on functional annotation
of 60,770 full-length cDNAs
JOURNAL Nature 420, 563-573 (2002)
REFERENCE 5
AUTHORS
CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the
FANTOM Consortium
TITLE Functional annotation of a full-length mouse cDNA collection
JOURNAL Nature 409, 685-690 (2001)
REFERENCE 6
AUTHORS Carninci,P. and Hayashizaki,Y.
TITLE High-efficiency full-length cDNA cloning
JOURNAL Meth. Enzymol. 303, 19-44 (1999)
REFERENCE 7
AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
TITLE Normalization and subtraction of cap-trapper-selected cDNAs to
prepare full-length cDNA libraries for rapid discovery of new genes
JOURNAL Genome Res. 10, 1617-1630 (2000)
REFERENCE 8
AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N.,
Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T.,
Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M.,
Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S.,
Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y.,
Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K.,
Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M.,
Inoue,Y., Kira,A. and Hayashizaki,Y.
TITLE RIKEN integrated sequence analysis (RISA) system-384-format
sequencing pipeline with 384 multicapillary sequencer
JOURNAL Genome Res. 10, 1757-1771 (2000)
COMMENT cDNA library was prepared and sequenced in Mouse Genome
Encyclopedia Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in RIKEN.
Division of Experimental Animal Research in Riken contributed to
prepare mouse tissues.
Please visit our web site for further details.
URL:http://www.osc.riken.jp/
URL:http://fantom.gsc.riken.jp/
clone information is available at:
http://fantom.gsc.riken.jp/3/db/annotate/
main.cgi?masterid=6030463A22
FEATURES Location/Qualifiers
source 1..2375
/clone="6030463A22"
/clone_lib="RIKEN full-length enriched mouse cDNA library"
/db_xref="FANTOM_DB:6030463A22"
/db_xref="MGI:2394876"
/db_xref="taxon:10090"
/dev_stage="13 days embryo"
/mol_type="mRNA"
/organism="Mus musculus"
/sex="male"
/strain="C57BL/6J"
/tissue_type="testis"
CDS 78..1184
/codon_start=1
/note="LEFT-RIGHT DETERMINATION FACTOR B PRECURSOR
(LEFTY-2 PROTEIN) (SWISSPROT|P57785, evidence: FASTY,
99.7%ID, 100%length, match=1104)"
/note="putative"
/protein_id="BAC27483.1"
/transl_table=1
/translation="MKSLWLCWALWVLPLAGPGAAMTEEQVLSSLLQQLQLSQAPTLD
SADVEEMAIPTHVRSQYVALLQGSHADRSRGKRFSQNFREVAGRFLMSETSTHLLVFG
MEQRLPPNSELVQAVLRLFQEPVPRTALRRFERLSPHSARARVTIEWLRVREDGSNRT
ALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSAHKL
VRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEVPVTEGTRCCRQEMYLDLQG
MKWAENWILEPPGFLTYECVGSCLQLPESLTIGWPFLGPRQCVASEMTSLPMIVSVKE
GGRTRPQVVSLPNMRVQTCSCASDGALIPRGIDL"
BASE COUNT 570 a 582 c 658 g 565 t
ORIGIN
1 gagaccgtct aggtcccaag aacttttcag ggcactttta gggacgcata tatccacgat
61 tcctcctggg cagcgccatg aagtccctgt ggctttgctg ggcactctgg gtactgcccc
121 tggctggccc tggggcagcg atgaccgagg aacaggtcct gagcagtcta ctgcagcagc
181 tgcagctcag ccaggccccc accctggaca gcgcggatgt ggaggagatg gccatcccta
241 cccacgtgag gtcccagtat gtggccctgc tgcagggaag tcacgctgac cgctcccgag
301 gcaagaggtt cagccagaat tttcgagagg tggcaggcag gttcctgatg tcagagacct
361 ccactcacct gctagtgttc ggaatggagc agcggctgcc gcctaacagc gagctggtgc
421 aggctgtgct gcggctgttc caggagcctg tgcccagaac agctctccgg aggtttgaga
481 ggctgtcccc acacagtgcc cgggctcggg tcaccattga atggctgaga gtccgtgagg
541 atggctccaa tcgcactgcc ctcatcgact ctaggctcgt gtccatccac gagagcggct
601 ggaaggcctt cgacgtgacc gaggccgtga acttctggca gcagctgagc cggccgaggc
661 agccgctgct gctccaggtg tcggtgcaga gggagcatct ggggccgggg acctggagcg
721 cacacaagtt ggtccgtttc gcggcgcagg ggacgccgga cggcaagggg cagggcgagc
781 cacagctgga gctgcacacg ctggacctca aggactacgg agctcaaggc aattgtgacc
841 ccgaggtacc agtgactgaa ggcacccgat gctgtcgcca ggagatgtac ctggacctgc
901 aggggatgaa gtgggccgag aactggatcc tagaaccgcc agggttcctg acgtatgaat
961 gtgtgggcag ctgcctgcag ctaccagagt ccctgaccat cgggtggcca tttctggggc
1021 ctcggcagtg tgttgcctca gagatgacct ccttgcccat gattgtcagt gtgaaggagg
1081 gaggcaggac caggcctcaa gtggtcagcc tgcccaacat gagggtgcag acctgtagct
1141 gcgcctcaga tggggcgctc atacccaggg ggatagatct gtagtctccc tgtccacaga
1201 tgtattctca gtgagcttgt cctaacttag tgctctcgtc agacctttgc tctacagtct
1261 tggttttctt gtccatcacc cagtttaagc acttacatgg gtaaatcatg tcactccagt
1321 aggacacact gaccccactt agccaaggac atggctatgc agtgaacagg ttcgcatctg
1381 agtctgtttt ctggccagaa ctcagcttaa tgtacaacaa aaccctacgg tgagaacagg
1441 ggaatcaaaa gctcgtttac tcttacaccg tgattactgg catcaacgta ccatgtcagg
1501 gactgcccac agcaggctgg gagggagaca tctcagaagc ctgcggcagc tccttgtgaa
1561 aaaccgttgt tcccatttct cctaacctta gccctagaca agagctgtat agatttcatg
1621 tgtgtgactg cttttcagtt ggccttggtg ttcatagtta ttctatatta tttgactttc
1681 ctactccttt ctccttctgc cctggtgaat tctatgaaac tagatgttcc ttgatgtaat
1741 gattcttaaa caattaaaaa gttgaggcat gggacacagc acagcacagt cctgatggcc
1801 caggtgcatg ctgtagatgt attctgtgtg ctcttatctt ggaaacaatg caataacttt
1861 gcaatgttag ttcagattaa tgtttgactt gcaaagaaag tttgaagaaa ttattagaaa
1921 gtgaaataga gccaacactg ggatcccgaa aagaaaaaag ctattgaagt tatgaaataa
1981 gttttgcaca aaatttgaga gtgtttcctg gataagcaag tatagaatac ataaaatctt
2041 atattagtaa aactaagcca aaacaccggg actcttagga gggtcactgc gtgcaatgtg
2101 cagaagcaga aagctggcag aactgccgag ttaagggtgt acctgagtct ttctggccat
2161 tacctggcag ctttgcccat gtcatttatt gtcagagctt cacgggaaaa tgcaagtagc
2221 cgacttcgga gctctgagct ctggagtata ataagtcaaa aggtaaagtt taaataatga
2281 taagtttgca ataattatta ttttggccag aggcctggga ataggggaag cttgaaactc
2341 tgggggaaca attataattc ttgattcttt gtgtg
//