LOCUS AK027703 2617 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ14797 fis, clone NT2RP4001256, highly similar
to Gamma-tubulin complex component 4.
ACCESSION AK027703
VERSION AK027703.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2617)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Ishibashi,T., Fujimori,K.,
Tanai,H., Kimata,M., Watanabe,M., Hiraoka,S., Ishii,S., Kawai,Y.,
Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y., Nagahari,K.,
Masuho,Y. and Kanehori,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction,
5'- & 3'-end one pass sequencing and clone selection: Helix
Research Institute (supported by Japan Key Technology Center etc.)
and Department of Virology, Institute of Medical Science,
University of Tokyo.
FEATURES Location/Qualifiers
source 1..2617
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP4001256"
/clone_lib="NT2RP4"
/db_xref="H-InvDB:HIT000010975"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 255..2258
/codon_start=1
/note="highly similar to Gamma-tubulin complex component
4"
/protein_id="BAG51368.1"
/translation="MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNR
LCRLGTDYIRFTEFIEQYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQ
ALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKH
SCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGNVSAQP
EEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKI
LFVGESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRS
TVAEHLWKLMVEESDLLGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDV
NVAFQQSAHKVLLDDDNLLPLLHLTIEYHGKEHKADATQAREGPSRETSPREAPASGW
AALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLKSN
QTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLS
NLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSS
LLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM"
BASE COUNT 658 a 663 c 635 g 661 t
ORIGIN
1 agtctccgca gagcccgggc gggagtagct ggtggacccc gttgagctgc cgaacttccg
61 ggactccccc gcgacccctt cccagcttcc cgtccgctcc gccgcagcga ttgtctcggt
121 gggttgattc ggcacaaacc gcccgaccca ggggccggtg cgcgtgtgga aggggaagca
181 ctcccctcgt ggtcgcctgg aggtgcgctg gaggaggggg tgacataacc agggactcga
241 ggtccgccgt gggaatgatc cacgaactgc tcttggctct gagcgggtac cctgggtcca
301 ttttcacctg gaacaagcgg agtggcctgc aggtatcgca ggacttccct ttcctccacc
361 ccagtgagac cagtgtcctg aatcgactct gccggctcgg cacagactat attcgcttca
421 ctgagttcat tgaacagtac acgggccatg tgcaacagca ggatcaccat ccatctcaac
481 agggccaagg tgggttacat ggaatctacc tgcgggcctt ctgcacaggg ctggattctg
541 ttttgcagcc ttatcgccaa gcactgcttg atttggaaca agagttcctg ggtgatcccc
601 atctctccat atcacatgtc aactacttcc tagaccagtt ccagcttctt tttccctctg
661 tgatggttgt agtagaacaa attaaaagtc aaaagattca tggttgtcaa atcctggaaa
721 cagtctacaa acacagctgt ggggggttgc ctcctgttcg aagtgcactg gaaaaaatcc
781 tggccgtttg tcatggggtc atgtataaac agctctcagc ctggatgctc catggactcc
841 tcttggacca gcatgaagaa ttctttatca aacaggggcc atcttctggt aatgtcagtg
901 cccagccaga agaggacgag gaggatctgg gcattggggg actgacagga aaacaactga
961 gagaactgca ggacttgcgc ctgattgagg aagagaacat gctggcacca tctctgaagc
1021 agttttccct acgagtggag attttgccat cctacattcc agtgagggtt gctgaaaaaa
1081 tcctatttgt tggagaatct gtccagatgt ttgagaatca aaatgtgaac ctgactagaa
1141 aaggatccat tttgaaaaac caggaagaca cttttgctgc agagctgcac cgtctcaagc
1201 agcagccact cttcagcttg gtggactttg aacaggtggt ggatcgcatt cgcagcactg
1261 tggctgagca tctctggaag ttgatggtag aagaatccga tttactgggt cagctgaaga
1321 tcattaaaga cttttacctt ctgggacgtg gagaactgtt tcaggccttc attgacacag
1381 ctcaacacat gttgaaaaca ccacccactg cagtaactga gcatgatgtg aatgtggcct
1441 ttcaacagtc agcacacaag gtattgctag atgatgacaa ccttctccct ctgttgcact
1501 tgacaatcga gtatcacgga aaggagcaca aagcagatgc tactcaggca agagaagggc
1561 cttctcggga aacttctccc cgggaagccc ctgcatctgg ctgggcagcc ctaggtcttt
1621 cctacaaagt acagtggcca ctacatattc tcttcacccc agctgtcctg gaaaagtaca
1681 atgttgtttt taagtactta ctgagtgtgc gccgggtgca agctgagctg cagcactgct
1741 gggccctaca aatgcagcgc aagcacctca agtcgaacca gactgatgca atcaagtggc
1801 gcctaagaaa tcacatggca tttttggtgg ataatcttca gtactatctc caggtagatg
1861 tgttggagtc tcagttctcc cagctgcttc atcagatcaa ttctacccga gactttgaaa
1921 gcatccgatt ggctcatgac cacttcctga gcaatttgct ggctcaatcc tttatcctat
1981 tgaaacctgt gtttcactgc ctgaatgaaa tcctagatct ctgtcacagt ttttgttcgc
2041 tggtcagtca gaacctaggc ccactggatg agcgtggagc cgcccagctg agcattctcg
2101 tgaagggctt tagccgccag tcttcactcc tgttcaagat tctctccagt gttcggaatc
2161 atcagatcaa ctcagatttg gctcaactac tgttacgact agattataac aaatactata
2221 cccaggctgg tggaactctg ggcagtttcg ggatgtgaaa atttctggct cataaattga
2281 aataacagcc acgttcccaa ggttgtaaca gaagattcaa aacatcccat tctagccaca
2341 cacaaataaa tatctgcggc ttagtgatag gactctacct tttctcctag aagcagttac
2401 tgaacatcca ggagtacaac tccttcccat cattcccatg tggaagggtc tctcccatca
2461 aggagaacat gtggcatctc tgatccttta cattgagaac atttgttgga tatgttcatt
2521 tattcaatag tcatttattg agcacctact acgtaccttg gtactgttca agctgtggga
2581 gatacagcgg taaacaaaca atatagagca gaaagtt
//