LOCUS AK027520 2018 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ14614 fis, clone NT2RP1001247, highly similar
to Transforming growth factor beta-4 precursor.
ACCESSION AK027520
VERSION AK027520.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2018)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction,
5'- & 3'-end one pass sequencing and clone selection: Helix
Research Institute (supported by Japan Key Technology Center etc.)
and Department of Virology, Institute of Medical Science,
University of Tokyo.
FEATURES Location/Qualifiers
source 1..2018
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP1001247"
/clone_lib="NT2RP1"
/db_xref="H-InvDB:HIT000010792"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pUC19FL3"
/note="mRNA from NT2 neuronal precursor cells after
48-hours retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 81..1181
/codon_start=1
/note="highly similar to Transforming growth factor
beta-4 precursor"
/protein_id="BAG51336.1"
/translation="MWPLWLCWALWVLPLAGPGAALTEEQLLGSLLRQLQLSEVPVLD
RADMEKLVIPAHVRAQYVVLLRRSHGDRSRGKRFSQSFREVAGRFLASEASTHLLVFG
MEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSAQARVTVEWLRVRDDGSNRT
SLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPLASGAHKL
VRFASQGAPAGLGEPQLELHTLDLRDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGMK
WAKNWVLEPPGFLAYECVGTCQQPPEALAFNWPFLGPRQCIASETASLPMIVSIKEGG
RTRPQVVSLPNMRVQKCSCASDGALVPRRLQP"
BASE COUNT 407 a 602 c 589 g 420 t
ORIGIN
1 acacccagct gcctgagacc ctccttcaac ctccctagag gacagcccca ctctgcctcc
61 tgctccccca gggcagcacc atgtggcccc tgtggctctg ctgggcactc tgggtgctgc
121 ccctggctgg ccccggggcg gccctgaccg aggagcagct cctgggcagc ctgctgcggc
181 agctgcagct cagcgaggtg cccgtactgg acagggccga catggagaag ctggtcatcc
241 ccgcccacgt gagggcccag tatgtagtcc tgctgcggcg cagccacggg gaccgctccc
301 gcggaaagag gttcagccag agcttccgag aggtggccgg caggttcctg gcgtcggagg
361 ccagcacaca cctgctggtg ttcggcatgg agcagcggct gccgcccaac agcgagctgg
421 tgcaggccgt gctgcggctc ttccaggagc cggtccccaa ggccgcgctg cacaggcacg
481 ggcggctgtc cccgcgcagc gcccaggccc gggtgaccgt cgagtggctg cgcgtccgcg
541 acgacggctc caaccgcacc tccctcatcg actccaggct ggtgtccgtc cacgagagcg
601 gctggaaggc cttcgacgtg accgaggccg tgaacttctg gcagcagctg agccggcccc
661 ggcagccgct gctgctacag gtgtcggtgc agagggagca tctgggcccg ctggcgtccg
721 gcgcccacaa gctggtccgc tttgcctcgc agggggcgcc agccgggctt ggggagcccc
781 agctggagct gcacaccctg gacctcaggg actatggagc tcagggcgac tgtgaccctg
841 aagcaccaat gaccgagggc acccgctgct gccgccagga gatgtacatt gacctgcagg
901 ggatgaagtg ggccaagaac tgggtgctgg agcccccggg cttcctggct tacgagtgtg
961 tgggcacctg ccagcagccc ccggaggccc tggccttcaa ttggccattt ctggggccgc
1021 gacagtgtat cgcctcggag actgcctcgc tgcccatgat cgtcagcatc aaggagggag
1081 gcaggaccag gccccaggtg gtcagcctgc ccaacatgag ggtgcagaag tgcagctgtg
1141 cctcggatgg ggcgctcgtg ccaaggaggc tccagccata ggcgcctggt gtatccattg
1201 agccctctaa ctgaacgtgt gcatagaggt ggtcttaatg taggtcttaa ctttatactt
1261 agcaagttac tccatcccaa tttagtgctc ctgtgtgacc ttcgccctgt gtccttccat
1321 ttcctgtctt tcccgtccat cacccatcct aagcacttac gtgagtaaat aatgcagctc
1381 agatgctgag ctctagtagg aaatgctggc atgctgatta caagatacag ctgagcaatg
1441 cacacatttt cagctgggag tttctgttct ctggcaaatt cttcactgag tctggaacaa
1501 taatacccta tgattagaac tggggaaaca gaactgaatt gctgtgttat atgaggaatt
1561 aaaaccttca aatctctatt tcccccaaat actgacccat tctggacttt tgtaaacata
1621 cctaggcccc tgttcccctg agagggtgct aagaggaagg atgaagggct tcaggctggg
1681 ggcagtggac agggaattgg gatacctgga ttctggttct gacagggcca caagctagga
1741 tctctaacaa acgcagaagg ctttggctcg tcatttcctc ttaaaaagga ggagctgggc
1801 ttcagctcta agaacttcat tgccctgggg atcagacagc ccctacctac ccctgcccac
1861 tcctctggag actgagcctt gcccgtgcat atttaggtca tttcccacac tgtcttagag
1921 aacttgtcac cagaaaccac atgtatttgc atgttttttg ttaatttagc taaagcaatt
1981 gaatgtagat actcagaaga aataaaaaat gatgtttc
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