LOCUS AK023227 2609 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ13165 fis, clone NT2RP3003686.
ACCESSION AK023227
VERSION AK023227.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2609)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2609
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3003686"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000006501"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 274..2508
/codon_start=1
/protein_id="BAB14478.1"
/translation="MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYD
ACLQHLARLQDINKDDYKIILNTAVAEFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMD
GLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFIEPFEEKFAQAVCFLLVDLYI
LTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGALIEAAKSKI
HQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSS
NIAEHPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGS
TDPGKKFSGRPMCTLLTNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLW
LRLAECCIAANKGTSEQETKGLPSKKGIVQSIVGQGYHRKIVLASQSIQNTVYNDGQS
SAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQLGGNTESSESSETCSSKS
HDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLLQQPKLS
GSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENETME
SSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEA
ILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMPAFTT
VQRK"
BASE COUNT 790 a 567 c 607 g 645 t
ORIGIN
1 agtgggtacc gggacgccgt gaggcggaag ctgtgtatgg cgggaggctg tggcggtccc
61 ttggtgggga agctgttgct gttgctagac gacgggaact agctctcgtc acttcctcag
121 cccgccgtct gcccactcct ctagccggaa cctgggggcc cggagccggg gtaggcacag
181 agttgtcctc ggaggtccag gacagcggcc agcccggcgg cgggagtcag ggccacgcca
241 cctgcaggga agaacccgag tcgaagcggg aagatggctg cagacaagcc tgcagatcag
301 ggagcagaga aacatgaagg cacaggtcag tcctctggga tcactgatca agagaaggag
361 ttatccacca atgctttcca agctttcaca tctggaaatt atgatgcctg tctacaacac
421 cttgcccgtc tacaagatat aaacaaagat gattataaaa taattttgaa tacagcagta
481 gctgagtttt ttaaaagtaa ccaaacaaca acagataatt tgagacaaac acttaaccag
541 ctgaagaatc aggtccactc agctgttgaa gaaatggatg gattagatga tgttgaaaac
601 agcatgttgt actataatca agcagtcatt ctttatcatc tgcggcagta tacagaagcc
661 atatcagttg gtgaaaaact ttatcagttc atagagcctt ttgaagaaaa atttgcccaa
721 gcagtgtgtt ttttgcttgt agacctgtat atattaacct accaagctga gaaagctttg
781 catcttcttg ctgtcctaga aaaaatgatt tcacagggta acaataacaa aaatggaaag
841 aatgagactg gtaataacaa caacaaagat ggatctaatc ataaagctga aagtggagct
901 ctaatagaag ctgcaaaatc aaagatacat cagtacaaag tacgagctta tatccaaatg
961 aagtctctga aagcatgtaa aagggaaatc aagtcagtca tgaatacagc tggaaattcc
1021 gcaccctctc tctttcttaa aagcaatttt gagtacttaa gaggtaatta tcgaaaagcc
1081 gtgaagctat taaatagttc aaacattgct gagcatccag gattcatgaa aacaggtgaa
1141 tgcttgagat gcatgttctg gaataacctt ggttgcatcc attttgccat gagcaagcac
1201 aatttgggaa tattctactt taaaaaggct ctgcaagaga atgacaatgt ctgtgcacag
1261 ctcagtgcag gtagcactga tccaggtaaa aaattttcag gaagacccat gtgtacgtta
1321 ctaaccaata agagatatga gttgctgtat aactgtggaa ttcagcttct tcacattgga
1381 aggcctcttg ctgccttcga atgtctgatt gaagctgttc aggtttatca tgcaaatcct
1441 cgcctctggc tacggctggc tgaatgctgc attgctgcca ataaggggac ttctgaacaa
1501 gaaactaaag gccttcccag caaaaaagga attgtacagt ctattgttgg tcaaggctat
1561 catcgtaaaa tagttttggc atcacagtct atacagaata ctgtttataa tgatgggcag
1621 tcttcggcca ttcctgtagc cagtatggag tttgcagcca tatgtctcag aaatgccttg
1681 ttgctgctac ctgaagaaca gcaagatcca aagcaggaaa atggggctaa aaatagtaat
1741 caattaggtg ggaacacaga gagcagcgaa agcagtgaaa cttgcagcag taaaagccat
1801 gatggagata aattcattcc agctccacct tcttctccat tgagaaaaca ggaattagaa
1861 aacttaaagt gctccatact tgcttgcagt gcctacgtgg ctctggcttt gggtgataac
1921 ctcatggctt tgaatcatgc agataaactt cttcagcagc ccaagctgtc aggatctctt
1981 aagtttttgg gacatttata tgctgcagaa gccctcatct ctctcgacag aatatctgat
2041 gccattactc acttgaaccc ggagaatgtc actgatgtct ccttagggat ctcttcaaat
2101 gagcaggacc aaggatcaga caaaggtgaa aatgaaacaa tggaatcctc tggtaagcgg
2161 gcccctcagt gctaccccag ttccgtcaac tctgccagga ctgtgatgct gttcaacctt
2221 ggcagcgctt actgcctgag gagcgaatat gacaaagccc gaaagtgtct ccaccaggcg
2281 gcttcaatga tccatcctaa agaggtgccc cctgaggcca tcttgctggc agtctacctt
2341 gaactgcaga atggtaatac tcagctggcc ttacagatca tcaaaaggaa tcagctgctc
2401 cctgcagtga aaacacactc tgaagtgaga aagaagccag tgtttcagcc tgtccacccg
2461 atccagccca tccaaatgcc ggctttcacc actgtgcaga gaaagtgata cttcactttt
2521 ggaaaactgt tacctgagac ccaggggaga atttactggc cattttagtt gtatcacagc
2581 agaatgaata aaagatggtg aaggctgtt
//