LOCUS AK023019 3166 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12957 fis, clone NT2RP2005531, weakly similar
to PROTEIN 4.1.
ACCESSION AK023019
VERSION AK023019.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3166)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Sasaki,N.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..3166
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2005531"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006293"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 143..2206
/codon_start=1
/protein_id="BAB14360.1"
/translation="MLSFFRRTLGRRSMRKHAEKERLREAQRAATHIPAAGDSKSIIT
CRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTK
SIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLDCPFDTAV
QLAAYNLQAELGDYDLAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAET
NYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGDTKIGLFFWPKITRLDF
KKNKLTLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRGPVQKSSH
RSGFIRLGSRFRYSGKTEYQTTKTNKARRSTSFERRPSKRYSRRTLQMKACATKPEEL
SVHNNVSTQSNGSQQAWGMRSALPVSPSISSAPVPVEIENLPQSPGTDQHDRKCIPLN
IDLLNSPDLLETTIGDVIGASDTMETSQALNDVNVATRLPGLGEPEVEYETLKDTSEK
LKQLEMENSPLLSPRSNIDVNINSQEEVVKLTEKCLNNVIESPGLNVMRVPPDFKSNI
LKAQVEAVHKVTKEDSLLSHKNANVQDAATNSAVLNENNVPLPKESLETLMLITPADS
GSVLKEATDELDALLASLTENLIDHTVAPQVSSTSMITPRWIVPLWSHFGRRSCPEAE
VFTDH"
BASE COUNT 922 a 671 c 699 g 874 t
ORIGIN
1 gtcctccggg gattagagcc ggtgggctcg ttgtgggcgc catttctcgg cgtctcccga
61 ggagccgccc ctttctcagc cttgctcggc tcttccccgc tctggtcgcc ggggctgcgc
121 cgtccccagc tcagtgacaa aaatgctgag tttcttccgt agaacactag ggcgtcggtc
181 tatgcgtaaa catgcagaga aggaacgact ccgagaagca caacgcgccg ccacacatat
241 tcctgcagct ggagattcta agtccatcat cacgtgtcgg gtgtcccttc tggatggtac
301 tgatgttagt gtggacttgc caaaaaaagc caaaggacaa gagttgtttg atcagattat
361 gtaccacctg gacctgattg aaagcgacta ttttggtctg agatttatgg attcagcaca
421 agtagcacat tggttggatg gtacaaaaag catcaaaaag caagtaaaaa ttggttcacc
481 ctattgtctg catcttcgag ttaagtttta ttcctcagaa ccaaataacc ttcgtgagga
541 gctaacccgg tatttatttg ttcttcagtt aaaacaagat attctcagtg gaaaattaga
601 ctgtcccttt gatacagcag tgcaattggc agcttataat ctgcaagctg aacttggtga
661 ctatgatctt gctgagcata gtcctgaact tgtctcagag ttcagattcg tgcctattca
721 gactgaagag atggaactgg ctatttttga gaaatggaag gaatacagag gtcaaacacc
781 agcacaggct gaaaccaatt atctgaataa agccaaatgg ctagaaatgt atggggttga
841 tatgcatgtg gtcaaggcta gagatgggaa tgactatagt ttgggactaa caccaacagg
901 agtccttgtt tttgaaggag ataccaaaat tggcttattt ttttggccga agataaccag
961 attggatttt aagaagaata aattaacctt ggtggttgta gaagatgatg atcagggcaa
1021 agaacaggaa catacatttg tctttagact ggatcatcca aaagcatgca aacatttatg
1081 gaaatgtgct gtggagcatc atgctttctt ccgccttcga ggccccgtcc aaaagagttc
1141 tcatcgatca ggatttattc gactaggatc acgatttaga tatagtggga aaacagagta
1201 tcagaccaca aaaaccaata aagcaagaag atcaacatcc tttgaaagaa ggcccagcaa
1261 acgatattct agacgaactc tacaaatgaa agcatgtgct acaaaacctg aagaacttag
1321 tgttcacaat aatgtttcga cccaaagtaa tggctcccaa caggcttggg ggatgagatc
1381 tgctctgcct gtgagtcctt ccatttcctc tgctcctgtg ccagtggaga tagagaatct
1441 tccacagagt cctggaacag accagcatga caggaaatgc attcctctga atattgattt
1501 gctgaatagc ccagacttat tggaaacaac gattggtgat gtaattgggg catctgacac
1561 tatggaaaca tcccaagcac tgaatgacgt taatgtagcc accaggcttc cgggattagg
1621 ggaacctgaa gttgaatatg agacattaaa agacacctca gagaagctca aacagcttga
1681 gatggagaac agtcctttgc tgtcccctcg atccaacatc gatgttaaca taaacagcca
1741 ggaggaagtg gtgaagttga ctgagaaatg ccttaataat gtcattgaga gcccaggatt
1801 gaatgtcatg agagttcctc ctgacttcaa gagtaacatt ttgaaggctc aagtagaagc
1861 agtgcataag gttacaaaag aagatagctt attaagtcat aaaaatgcca atgttcagga
1921 tgctgccaca aacagtgctg tgttaaatga gaataatgtg cccctcccca aagagtctct
1981 tgagactctg atgcttatca cacctgccga cagtggttct gttctaaagg aagctacaga
2041 tgaattggat gccttgcttg catctctaac tgagaatcta attgatcaca cagttgcacc
2101 tcaggtgtct tccacatcca tgatcacacc ccggtggatt gttccgctct ggtcccattt
2161 tggcagaaga agctgtcctg aagcagaagt gtttactgac cactgagctc tgagggcctg
2221 tagctggaat acgcatctct ccagcattcc gtcctgggat ccgtttcagc tagaatatgt
2281 tggattcagg agcttgtcca ttatttgtag gtaaaaaaag ctgcacgtag atttgacttc
2341 aactccgtaa aaaagacagc tgtattttcc gtccaactgg aattgttgaa tcacactgca
2401 tagctgccca aaagagagtg tttggtcttg aactttctat acttttataa atgttacaaa
2461 ttcccgaaag aagggaattt ctttttctgg ggtttccttc aaactcttgg ctccacctag
2521 cggttctatt tgttcataac aacttcataa caagcctgcc tctggtagtc aacagccttt
2581 tgaaagctat ttccatctag tatcagggtg agagcatcct tgatctggct gcctgttaga
2641 gaaattgcac ttttcctgac ttacctagaa atcaagaatt taggaaatta atgtggacac
2701 tataaaggca gacttagggc caactttttt tttttttaca attattacaa cactaaagag
2761 aagtttagaa tatagagagt ttttaaatgt ctcccattct tttgatttct tactgtactg
2821 gctatcttaa tatttcaagt ttacatcaag ataaaccctg agaagaacta cggagaaatc
2881 aaataaaatc ctgtcatatt tttttcaccc tgcctttcca caggaagcac tcacaggcac
2941 cacacacgta tcatgtaact tatcagtggg gtgggttact gttgaagaga ccctggggca
3001 tttacctcag gcatctgcac tcctccgagc ccggtggaga atgcaggctg ctgtagtctc
3061 aggtaatgaa ggcacagcac agcagtactc cacattgttt cctatttgga catagacttc
3121 atttcctttc agtataagct gagtaaattt agagctttca aactgg
//