LOCUS AK022952 2741 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12890 fis, clone NT2RP2004124.
ACCESSION AK022952
VERSION AK022952.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2741)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Takahashi-Fujii,A., Hara,H.,
Tanase,T., Nomura,Y., Togiya,S., Komai,F., Hara,R., Takeuchi,K.,
Arita,M., Nabekura,T., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Oshima,A.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2741
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2004124"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006226"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 256..2490
/codon_start=1
/protein_id="BAB14327.1"
/translation="MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYD
ACLQHLACLQDINKDDYKIILNTAVAEFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMD
GLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFIEPFEEKFAQAVCFLLVDLYI
LTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGALIEAAKSKI
HQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSS
NIAEHPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGS
TDPGKKFSGRPMCTLLTNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLW
LRLAECCIAANKGTSEQETKGLPSKKGIVQSIVGQGYHRKIVLASQSIQNTVYNDGQS
SAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQLGGNTESSESSETCSSKS
HDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLLQQPKLS
GSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAME
SSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEA
ILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMPAFTT
VQRK"
BASE COUNT 835 a 589 c 619 g 698 t
ORIGIN
1 gtgaggcgga agctgtgtat ggcgggaggc tgtggcggtc ccttggtggg gaagctgttg
61 ctgttgctag acgacgggaa ctagctctcg tcacttcctc agcccgccgt ctgcccactc
121 ctctagccgg aacctggggg cccggagccg gggtaggcac agagttgtcc tcggaggtcc
181 aggacagcgg ccagcccggc ggcgggagtc agggccacgc cacctgcagg gaagaacccg
241 agtcgaagcg ggaagatggc tgcagacaag cctgcagatc agggagcaga gaaacatgaa
301 ggcacaggtc agtcctctgg gatcactgat caagagaagg agttatccac caatgctttc
361 caagctttca catctggaaa ttatgatgcc tgtctacaac accttgcctg tctacaagat
421 ataaacaaag atgattataa aataattttg aatacagcag tagctgagtt ttttaaaagt
481 aaccaaacaa caacagataa tttgagacaa acacttaacc agctgaagaa tcaggtccac
541 tcagctgttg aagaaatgga tggattagat gatgttgaaa acagcatgtt gtactataat
601 caagcagtca ttctttatca tctgcggcag tatacagaag ccatatcagt tggtgaaaaa
661 ctttatcagt tcatagagcc ttttgaagaa aaatttgccc aagcagtgtg ttttttgctt
721 gtagacctgt atatattaac ctaccaagct gagaaagctt tgcatcttct tgctgtccta
781 gaaaaaatga tttcacaggg taacaataac aaaaatggaa agaatgagac tggtaataac
841 aacaacaaag atggatctaa tcataaagct gaaagtggag ctctaataga agctgcaaaa
901 tcaaagatac atcagtacaa agtacgagct tatatccaaa tgaagtctct gaaagcatgt
961 aaaagggaaa tcaagtcagt catgaataca gctggaaatt ccgcaccctc tctctttctt
1021 aaaagcaatt ttgagtactt aagaggtaat tatcgaaaag ccgtgaagct attaaatagt
1081 tcaaacattg ctgagcatcc aggattcatg aaaacaggtg aatgcttgag atgcatgttc
1141 tggaataacc ttggttgcat ccattttgcc atgagcaagc acaatttggg aatattctac
1201 tttaaaaagg ctctgcaaga gaatgacaat gtctgtgcac agctcagtgc aggtagcact
1261 gatccaggca aaaaattttc aggaagaccc atgtgtacgt tactaaccaa taagagatat
1321 gagttgctgt ataactgtgg aattcagctt cttcacattg gaaggcctct tgctgccttc
1381 gaatgtctga ttgaagctgt tcaggtttat catgcaaatc ctcgcctctg gctacggctg
1441 gctgaatgct gcattgctgc caataagggg acttctgaac aagaaactaa aggccttccc
1501 agcaaaaaag gaattgtaca gtctattgtt ggtcaaggct atcatcgtaa aatagttttg
1561 gcatcacagt ctatacagaa tactgtttat aatgatgggc agtcttcggc cattcctgta
1621 gccagtatgg agtttgcagc catatgtctc agaaatgcct tgttgctgct acctgaagaa
1681 cagcaagatc caaagcagga aaatggggct aaaaatagta atcaattagg tgggaacaca
1741 gagagcagcg aaagcagtga aacttgcagc agtaaaagcc atgatggaga taaattcatt
1801 ccagctccac cttcttctcc attgagaaaa caggaattag aaaacttaaa gtgctccata
1861 cttgcttgca gtgcctacgt ggctctggct ttgggtgata acctcatggc tttgaatcat
1921 gcagataaac ttcttcagca gcccaagctg tcaggatctc ttaagttttt gggacattta
1981 tatgctgcag aagccctcat ctctctcgac agaatatctg atgccattac tcacttgaac
2041 ccggagaatg tcactgatgt ctccttaggg atctcttcaa atgagcagga ccaaggatca
2101 gacaaaggtg aaaatgaagc aatggaatcc tctggtaagc gggcccctca gtgctacccc
2161 agttccgtca actctgccag gactgtgatg ctgttcaacc ttggcagcgc ttactgcctg
2221 aggagcgaat atgacaaagc ccgaaagtgt ctccaccagg cggcttcaat gatccatcct
2281 aaagaggtgc cccctgaggc catcttgctg gcagtctacc ttgaactgca gaatggtaat
2341 actcagctgg ccttacagat catcaaaagg aatcagctgc tccctgcagt gaaaacacac
2401 tctgaagtga gaaagaagcc agtgtttcag cctgtccacc cgatccagcc catccaaatg
2461 ccggctttca ccactgtgca gagaaagtga tacttcactt ttggaaaact gttacctgag
2521 acccagggga gaatttactg gccattttag ttgtatcaca gcagaatgaa taaaagatgg
2581 tgaaggctgt taattttgag tcaattctac ccctgacttt tggccaaaag cttacttaaa
2641 attaaggatt tactaagtca tcatcagctg tttttcttaa tttcagccag actattaatt
2701 ttgagccatt atgtaatata tgccataggc aattaaaaca t
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