LOCUS AK022950 3413 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12888 fis, clone NT2RP2004081.
ACCESSION AK022950
VERSION AK022950.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3413)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Takahashi-Fujii,A., Hara,H.,
Tanase,T., Nomura,Y., Togiya,S., Komai,F., Hara,R., Takeuchi,K.,
Arita,M., Nabekura,T., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Oshima,A.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..3413
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2004081"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006224"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 333..2210
/codon_start=1
/protein_id="BAB14325.1"
/translation="MNVTSIALRAGTWLLAAWHVKVPPMWLEACINWIQEENNNVNLS
QAQMNKQVFEQWLLTDLRDLEHPLLPDGILEIPKGELNGFYALQINSLVDVSQPAYSQ
IQKLRGKNTTNDLVTAEAQVTPKPWEAKPSRMLMLQLTDGIVQIQGMEYQPIPILHSD
LPPGTKILIYGNISFRLGVLLLKPENVKVLGGEVDALLEEYAQEKVLARLIGEPDLVV
SVIPNNSNENIPRVTDVLDPALGPSDEELLASLDENDELTTNNDTSSERCFTTGSSSN
TIPTRQSSFEPEFVISPRPKEEPSNLSIHVMDGELDDFSLEEALLLEETVQKEQMETK
ELQPLTFNRNADRSIERFSHNPNTTNNFSLTCKNGNNNWSEKNVSEQMTNEDKSFGCP
SVRDQNRSIFSVHCNVPLAHDFTNKEKNLETDNKIKQTSSSDSHSLNNKILNREVVNY
VQKRSSQISNENDCNLQSCSLRSSENSINLSIAMDLYSPPFVYLSVLMASKPKEVTTV
KVKAFIVTLTGNLSSSGGIWSITAKVSDGTAYLDVDFVDEILTSLIGFSVPEMKQSKK
DPLQYQKFLEGLQKCQRDLIDLCCLMTISFNPSLSKAMVLALQDVNMEHLENLKKRLN
K"
BASE COUNT 1089 a 574 c 657 g 1093 t
ORIGIN
1 aaaaaggaac tgggatcgcg ccgcggcggt tcctgtcctt acagttgcgc tgcccaggga
61 ccgatgttgc gcgaggaaaa tgcgggacgc ccaggtcggt gctcggccca gacttatgcc
121 cgtgttttca cagcccacac tcgtgcccga agccccttac cccgccccgg gctgtggtct
181 gtgggctgtg ggacgagctg cggcgcgggc catttctgag cagtggaggt ttcaagtaat
241 ccactaacaa ccagttccaa attctgtcat caaatcctgt gctgctgttc ctcgtggtaa
301 tagatgcata ttatttcttt tatttaaaag aaatgaatgt gactagtatt gcattaagag
361 ctggaacttg gcttttagct gcatggcatg ttaaagtacc tccgatgtgg ctggaagctt
421 gtattaactg gattcaagaa gaaaataata atgttaactt gagtcaggcc caaatgaata
481 aacaagtgtt tgagcagtgg ctccttactg atctgaggga tttggagcat cctcttttac
541 ccgatggcat tttagaaatt ccaaaaggag aattaaatgg attttatgct ctgcagatta
601 attccttggt tgatgtaagt cagcctgcat actcccagat acagaagttg agaggaaaga
661 atacaacaaa tgatctagtt acagctgaag cacaagtaac cccaaaacct tgggaagcaa
721 agccttcacg aatgttgatg ctgcagctaa ctgatggaat cgtacaaata cagggaatgg
781 aatatcagcc tattccaatt cttcatagtg atcttcctcc aggtacaaaa attttgattt
841 atggaaatat atctttccgt cttggtgttc tcttattgaa accagaaaac gtgaaagtgt
901 taggaggtga agtagatgct cttttagaag aatatgccca agaaaaagta cttgcaagat
961 taatagggga acctgatctt gtagtttcag tcataccaaa caattctaac gaaaacattc
1021 ccagagttac agatgttcta gatcctgcat taggtccttc tgatgaagaa ctcttggcaa
1081 gtcttgatga aaatgatgag cttacaacaa ataatgacac ttcctcagaa cgatgtttca
1141 ccacaggtag ttcctcaaat accattccca caagacagtc aagttttgag ccagaatttg
1201 ttatttctcc aagaccaaaa gaggaaccat caaacctatc tatacatgta atggatggag
1261 aattagatga cttttcactg gaggaggcct tgcttttaga agaaactgtc cagaaagaac
1321 agatggaaac taaggaattg caaccattga cttttaacag aaatgccgat cgaagtatag
1381 agagattttc acataatcct aatactacga ataacttttc tttgacttgc aaaaatggaa
1441 acaataattg gagtgaaaaa aatgtatctg aacaaatgac taatgaagac aaatcatttg
1501 gttgtccatc tgttagagac caaaacagga gtattttttc agttcattgt aatgtaccct
1561 tagcccatga ttttacaaat aaagaaaaga acttagagac agataataaa ataaaacaaa
1621 ccagcagttc agatagccat tccttaaata ataaaatatt aaatagagag gtggtcaact
1681 atgtacagaa aaggagttca caaatttcta atgaaaatga ttgtaattta cagagttgtt
1741 ctttaagatc atcagagaat agcattaatc tttctattgc catggatttg tattctccac
1801 cctttgtcta tttgtctgtt ctaatggcca gcaaaccaaa ggaagttaca acagtgaaag
1861 tgaaagcatt tattgtaacc ttaactggaa atctctcaag ttctggtggt atttggagta
1921 taactgcaaa ggtgtctgat ggtactgcat atctagatgt agactttgtg gatgaaatac
1981 ttactagctt gatagggttc tcagtaccag aaatgaaaca gtcaaaaaag gatcctcttc
2041 aataccaaaa gttcctggaa gggttgcaga aatgtcaaag agatctaata gatttgtgct
2101 gtctaatgac tatttcattt aatccttcct tgtctaaagc aatggtactg gcattacaag
2161 atgttaatat ggaacacctt gagaatctaa agaagcggtt aaataaataa ttaaactaaa
2221 atagtattag gaacaattaa aaacaacaag gaaatattta gaatttgttc acaattttac
2281 ttatgatact ttgtgtaaaa aggaaaaatg aaatttcata gcttatttca gtttaatttt
2341 aaagtgttta atcatgtttg ttatatgtgg agcttttgaa aataagttaa tctttttttt
2401 tttttttaat gtcagggtat tgctctgttg cccaggctag agtgcagtgg catgatcata
2461 ggttattgca tcaacctctt gagctcaagc gatcctcctg ccttagcctc ttgagttgct
2521 gggactatag gcatgtacca ccatgcccag ctaatttata attactatta ttgttttgta
2581 gggacagagt tttgctatgt tgcccagatt ggtctcaaac tcctggcctc tcactcctgc
2641 cgtcatggcc tccaaagttt aattttttta aaagtaactg ttagatggag gaatatggtg
2701 acccactatt gttgagaaat ttgttttcca cttgtcacgt cacataagat gattgctaaa
2761 atattgtact ggtgttcggt ggtgagtcat atttttgaaa gttaataatt ataaataggt
2821 aatttccttc taatatgttg gtactgtcta tggccatacc accctgaaca tgcctgagct
2881 tgtcataata tgttgagtac ccaaaagatt tgtttatatt gttaatctta gggaaaaaaa
2941 attaaaatcc agtagatcag aacatcaggc tttcagatac aaattgattt actggttttt
3001 attttgctga ttataatatt tggtatattt aaggtaatct agttaactag atgctatttc
3061 atagattata ttgaatgatt taaaacttta ttttcaagga tagtttattt taaatggcat
3121 attgaaaaca tcattattaa gatccagtag gtaggacatt tattggatta aaatgaagca
3181 tttatctatg tctttaggtg tcattgttcc ctttctgaat tagctgtaca tataagcctt
3241 cctttggttt taagtactga ttttttttta aaaaaagagg gactgtttac cattcttcca
3301 ctgtgctgtt ataaagttgt atttgaaagg taatgttgtt tttattaatc ttttgtctta
3361 aaataattta aagtgctttg aattttaaaa cattaaacaa atccttaaat aac
//