LOCUS AK022945 3046 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12883 fis, clone NT2RP2003981, weakly similar
to VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS8.
ACCESSION AK022945
VERSION AK022945.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3046)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Takahashi-Fujii,A., Hara,H.,
Tanase,T., Nomura,Y., Togiya,S., Komai,F., Hara,R., Takeuchi,K.,
Arita,M., Nabekura,T., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Oshima,A.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..3046
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2003981"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006219"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 86..2467
/codon_start=1
/protein_id="BAB14322.1"
/translation="MKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLY
DAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRA
YPLGDIPEDLVPLVKNQVFEFLIRLHSSEASPEEEIYPYIRTLLHFDTREFLNVLALT
FEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNR
TLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEF
MYEREHQYDKIIDCYLRDPLREEEVFNYIHNILSIPGHSAEEKQSVWQKAMDHIEELV
SLKPCKAAELVATHFSGHIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQELLQISPC
ITEQFIELLCQFNPAQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGA
FLIMLERLQSKLQEVTHQGENTKEDPSLKDVEDTMVETIALCQRNSHNLNQQQREALW
FPLLEAMMAPQKLSSSAIPHLHSGALKSLTMQVLNSMAAFIALPSILQRILQDPVYGK
GKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCS
ICLQQYKRRQEMADEIIVFSCGHLYHSFCLQNKECTVEFEGQTRWTCYKCSSSNKVGK
LSENSSEIKKGRITPSQVKMSPSYHQSKGDPTAKKGTSEPVLDPQQIQAFDQLCRLYR
GSSRLALLTELSQNRSSESYRPFSGSQSAPAFNSIFQNENFQLQLIPPPVTED"
BASE COUNT 891 a 671 c 650 g 834 t
ORIGIN
1 gaattcagtg gccaaaggag tatttttgga gtgccttgag ccatatattt taagtgataa
61 attggtggga atcacacccc aagtaatgaa agacttgatt gttcatttcc aagataaaaa
121 attaatggaa aatgtggaag cgctcattgt acatatggat atcaccagcc tagatattca
181 gcaggtagtt ctcatgtgtt gggaaaatcg tttatatgat gctatgatct atgtctacaa
241 cagaggcatg aatgaattta ttagtccaat ggagaaactt ttcagagtca ttgctcctcc
301 tctgaatgca ggaaaaacac taacagatga acaagttgtt atgggcaata agctccttgt
361 atatattagc tgttgtctag caggtcgtgc ctatcccctt ggtgacatcc ctgaagatct
421 ggttcccttg gttaaaaacc aggtttttga atttctaatt cgcctgcatt catcagaggc
481 ttctcctgag gaagaaatct atccttacat tcggactttg ctacattttg acacaagaga
541 atttctcaat gtattggcac tgacttttga agattttaaa aatgacaagc aagctgtgga
601 atatcaacag cgaattgtgg atattttgtt gaaagttatg gtggagaatt cagactttac
661 cccctcacaa gtaggatgtc tctttacctt ccttgctcgg cagcttgcaa agcctgacaa
721 caccttgttt gtaaacagaa cactttttga tcaggtcctt gaattccttt gtagtcctga
781 cgatgactcc cgacactctg aaagacagca ggtcctttta gaattgctgc aggctggagg
841 catagttcaa tttgaagaga gtcgactcat ccggatggca gaaaaagctg agttctatca
901 aatttgtgaa tttatgtatg aaagagaaca ccaatatgac aaaattattg attgctactt
961 acgtgaccct ctgcgagagg aagaagtctt taattacatt cacaatatct tatccattcc
1021 cggacacagt gcagaggaga agcagtctgt atggcagaaa gcaatggatc atattgagga
1081 actcgtgtcc ctgaagcctt gtaaagctgc ggagctggtt gccacccact tttctggaca
1141 tattgaaacg gtcattaaaa aacttcagaa ccaggttttg cttttcaaat ttttgaggag
1201 tcttcttgac ccaagggaag gtattcatgt aaatcaagaa ttactgcaaa tatctccttg
1261 tatcacagag cagttcattg agctgttgtg tcagttcaac ccagcccaag ttatagagac
1321 tctgcaagtc cttgagtgct accgtctgga agaaactatt cagattactc agaagtatca
1381 acttcatgaa gtcaccgctt atctattgga aaagaaagga gatattcatg gtgccttcct
1441 aataatgtta gagagactac aaagcaaact tcaagaggta acacatcaag gtgaaaatac
1501 caaagaggat ccctcattga aggatgttga agatactatg gtggagacca ttgctctttg
1561 ccagagaaat tcacataatt tgaaccagca gcaacgtgag gccctttggt tcccgttatt
1621 ggaggcaatg atggcccctc agaagctgtc cagttcagcc attcctcatc tacactctgg
1681 agctctgaag tctttgacca tgcaagtttt aaatagcatg gcagcattta ttgcccttcc
1741 atcaatcttg caaagaatct tacaggatcc agtttatgga aaaggaaaac ttggagaaat
1801 ccagggactt atcttgggaa tgttagatac ctttaactat gaacaaaccc tgctggaaac
1861 aacaaccagc cttctaaacc aagatctcca ttggtcattg tgtaacctga gagcttcggt
1921 caccagagga ctgaatccca aacaagatta ctgctctata tgtttgcagc agtacaagag
1981 acgccaagaa atggctgatg aaataattgt ctttagctgt ggccatttgt atcactcatt
2041 ctgcctacaa aacaaagaat gcactgtgga atttgagggc caaacaagat ggacatgcta
2101 caaatgcagt tcaagtaaca aagtaggaaa actcagtgaa aattcatctg aaattaaaaa
2161 gggaaggata accccatcac aggtaaaaat gtctccatcg tatcatcagt ccaaagggga
2221 tcccactgct aaaaagggaa cctcagaacc tgttctggat ccacagcaaa tccaagcatt
2281 tgatcagctt tgccgtctct accgaggaag ctccaggctg gctctcctca cggaactctc
2341 ccagaatcgc agcagcgaga gctataggcc attcagtggc tcgcagagtg ctcctgcttt
2401 caacagcatc ttccagaatg agaacttcca gctgcagctc attcctccac ctgtgactga
2461 ggattgatga ctccatggag cctggcccag gagaaccaga gatgatcccg aggcagctgg
2521 ggagaggccc cgcctctggt gggcttggcc tccaccacct cccacgcttc tgagaagagg
2581 ttccaaattg ggcttctgtg cccagagcgt ccacagcacc attcccagtg tagactccca
2641 gtcttctcca cattgctgtc atggcgtcag ttcaccagac tcattgattt tgttttgctt
2701 gttaagcaaa ggaatgtcac atacctctgt ccagcttttt aggaaataca tttcgcctat
2761 tgcgactttt tccatttacc ctgaagccta gaaagtaggt ggaactcaca caaatggcat
2821 tccagagtct gccatactcc gtctcctcca gctgctggat aatacagagg aacttcaact
2881 tctacaggga acagtggttg gccaggctgc agtataactg aagcatgcct tggagagagc
2941 agacactgtg ggggccaggg ccatctccct ttaatgtgtt catgttaaaa cctatttgag
3001 tgtaagactt gccctttcta acaataaatg ctccgtgttt aagttc
//