LOCUS AK022909 2518 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12847 fis, clone NT2RP2003347.
ACCESSION AK022909
VERSION AK022909.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2518)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Takahashi-Fujii,A., Hara,H.,
Tanase,T., Nomura,Y., Togiya,S., Komai,F., Hara,R., Takeuchi,K.,
Arita,M., Nabekura,T., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Oshima,A.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2518
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2003347"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006183"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 1..2223
/codon_start=1
/protein_id="BAB14304.1"
/translation="MNEHLSSLIKKKRKCMQPKDFNFKTPENDKRFQKKFEKMAKELQ
RQKTNLDDDVPILLFESNGSLIYTPTIEINSSHHSAMEKRLQEMKEKRENLSPTSSQM
IQQSHDNPSNSLCEAPLNISRDTLCSDEYFAGGLHSSFDDLCGNSGCGNQERKLEGSI
NDIKSDVCISSLVLKANNIHSSPSFTHLDKSSPQKFLSNLSKEEINLQRNIAGKVVTP
HQKQAAGMSQETFEEKYRLSPTLSSTKGHLLIHSRPRSSSVKRKRVSHGSHSPPKEKC
KRKRSTRRSIMPRLQLCRSEGRLQHVAGPALEALSCGESSYDDYFSPDNLKERYSENL
PPESQLPSSPAQLSCRSLSKKERTSIFEMSDFSCVGKKTRTVDITNFTAKTISSPRKT
GNGEGRATSSCVTSAPEEALRCCRQAGKEDACPEGNGFSYTIEDPALPKGHDDDLTPL
EGSLEEMKEAVGLKSTQNKGTTSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKENLP
GGYSGSVKNRPTRHDVLDDSCDGFKDLIKPHEELKKSGRGKKPTRTLVMTSMPSEKQN
VVIQVVDKLKGFSIAPDVCETTTHVLSGKPLRTLNVLLGIARGCWVLSYDWVLWSLEL
GHWISEEPFELSHHFPAAPLCRSECHLSAGPYRGTLFADQPVMFVSPASSPPVAKLCE
LVHLCGGRVSQVPRQASIVIGPYSGKKKATVKYLSEKWVLDSITQHKVCAPENYLLSQ
"
BASE COUNT 775 a 538 c 564 g 641 t
ORIGIN
1 atgaatgaac acttatcaag cctaattaaa aaaaaacgta aatgtatgca gcccaaagat
61 tttaatttta aaacaccaga aaatgataag agatttcaga agaaatttga gaaaatggct
121 aaagagctac aaaggcaaaa aacaaatcta gatgatgatg tacctattct cttatttgaa
181 tctaatggtt cattaatata tactcccaca attgaaatta atagtagtca ccacagcgca
241 atggagaaga gattacaaga gatgaaggag aaaagggaaa atctttcccc cacctcttcc
301 caaatgattc agcagtctca tgataatcca agtaactctc tgtgtgaagc acctttgaac
361 atttcacgtg atactttgtg ttcagatgaa tactttgctg gtggcttaca ctcatctttt
421 gatgatcttt gtggaaactc aggatgtgga aatcaggaaa ggaagttgga aggatccatt
481 aatgacatta aaagtgatgt gtgtatttct tcacttgtat tgaaagcaaa taatattcat
541 tcatcaccat ctttcactca cctcgataaa tcaagtcctc agaaatttct gagtaatctt
601 tcaaaggaag aaataaactt gcaaagaaat attgcaggta aagtagtcac ccctcaccaa
661 aagcaggctg caggtatgtc tcaggagacg tttgaagaga agtatcgttt gtctcctacc
721 ttatcttcaa caaaaggcca ccttttgata cattcaagac ccaggagttc ctcagtaaag
781 agaaaaagag tatcacatgg ctcccattca cctccgaagg aaaaatgcaa gagaaagagg
841 agcaccagga gatctatcat gccgaggctg cagctgtgca ggtcggaagg caggctgcag
901 cacgtggcgg gacctgccct ggaggctctt agctgtgggg agtcttcata tgatgactat
961 ttttcacctg ataatcttaa ggaaaggtat tcagagaatc ttcctcctga atctcagctg
1021 ccatcaagcc ctgctcagtt gagctgcaga agtctttcta agaaggagag aacaagcata
1081 tttgaaatgt ctgatttttc ctgcgttggc aaaaaaacca gaacagttga cattaccaat
1141 ttcacagcaa aaaccatctc cagtcctcgg aaaactggaa atggtgaagg ccgtgcaact
1201 tcgagttgcg tgacttctgc ccctgaagaa gccctaaggt gttgtagaca ggctgggaaa
1261 gaagacgcat gcccagaggg aaatggcttt tcttacacca ttgaggaccc tgctcttcca
1321 aaaggacatg atgatgattt aactcctttg gaaggaagcc ttgaagaaat gaaagaagcg
1381 gttggtctga aaagcacaca gaacaaaggt accacttcca aaatatcaaa ctcctctgaa
1441 ggcgaagccc agagtgaaca tgagccatgt tttatagttg actgtaacat ggagacgtct
1501 acagaagaga aggaaaactt acccggagga tacagtggaa gtgttaaaaa tagaccaaca
1561 aggcatgatg ttttagatga ctcatgtgac ggctttaagg acctcatcaa acctcatgag
1621 gaattgaaga aaagtgggag aggcaaaaag ccaacaagaa cattagtcat gacaagcatg
1681 ccatctgaaa agcagaatgt cgtcatccag gttgtggata aattgaaagg cttttcaatt
1741 gcaccagacg tctgtgagac cacgactcac gtgctttccg ggaagccact tcgcaccctg
1801 aatgtgctgc tgggaattgc gcgtggctgc tgggttctct cttatgattg ggtgctatgg
1861 tctttagaat tgggtcactg gatttctgag gagccgttcg aactgtctca ccacttccct
1921 gcagctcccc tgtgccgaag tgagtgccac ttgtctgcag ggccgtaccg cggaaccctc
1981 tttgccgacc agccagtgat gtttgtctcg cctgccagca gccccccagt ggccaagctc
2041 tgtgaactag tccacctgtg cggaggccgg gtcagccaag tcccccgcca ggccagcatc
2101 gtcatcgggc cctacagcgg aaagaagaaa gcaacagtca agtatctgtc tgagaaatgg
2161 gtcttagatt ccatcaccca gcacaaggtc tgtgcccctg aaaactacct attgtcacaa
2221 tgacagtgac ctcactggcc tgtggtgact gcacacagct cgcaaaactg tctttggatg
2281 ttcaaatgag aaacaaaact gtgaagagaa ggaactggcg tatacaagat gacttctgat
2341 atcatgtttg ccatgtgttg tggttcttaa gaactcatag gtgactttct gatgactgaa
2401 tgtctgtttc agagacgctt cgggcctttt tatttttatt ttatttttta ttttttgaga
2461 cggagtccag cctgggcaac agagtaactc tccttctcaa ataaataaat aaataaat
//