LOCUS AK022899 2860 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ12837 fis, clone NT2RP2003228, highly similar
to DNA replication licensing factor MCM4.
ACCESSION AK022899
VERSION AK022899.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2860)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Takahashi-Fujii,A., Hara,H.,
Tanase,T., Nomura,Y., Togiya,S., Komai,F., Hara,R., Takeuchi,K.,
Arita,M., Nabekura,T., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Oshima,A.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2860
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2003228"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006173"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 56..2647
/codon_start=1
/note="highly similar to DNA replication licensing factor
MCM4"
/protein_id="BAG51132.1"
/translation="MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTS
TGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGT
PRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVA
ACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSF
DKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNP
EDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTT
HSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTG
IYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLK
ELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILL
CGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLS
DNGICCIDEFDRMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQW
NPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELL
DMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRL
AEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRK
EELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVT
GKTVRLL"
BASE COUNT 766 a 706 c 724 g 664 t
ORIGIN
1 ggagtccgcg agcgtcgtcg gcaagcggcc gcctttccac ggtactccga gcactatgtc
61 gtccccggcg tcgaccccga gccgccgcgg cagccggcgt ggaagggcca cccccgccca
121 gacgcctcgg agtgaggatg ccaggtcatc tccctctcag agacgtagag gcgaggattc
181 cacctccacg ggggagttgc agccgatgcc aacctcgcct ggagtggacc tgcagagccc
241 tgctgcgcag gacgtgctgt tttccagccc tccccaaatg cattcttcag ctatccctct
301 tgactttgat gttagttcac cactgacata cggcactccc agctctcggg tagagggaac
361 cccaagaagt ggtgttaggg gcacacctgt gagacagagg cctgacctgg gctctgcaca
421 gaagggcctg caagtggatc tgcagtctga cggggcagca gcagaagata tagtggcaag
481 tgagcagtct ctaggccaaa aacttgtgat ctggggaaca gatgtaaatg tggcagcatg
541 caaagaaaac tttcagagat ttcttcagcg ttttattgac cctctggcta aagaagaaga
601 aaatgttggc atagatatta ctgaacctct atacatgcaa cgacttgggg agattaatgt
661 tattggtgag ccatttttaa atgtgaactg tgaacacatc aaatcatttg acaaaaattt
721 gtacagacaa ctcatctctt acccacagga agttattcca acttttgaca tggctgtcaa
781 tgaaatcttc tttgaccgtt accctgactc aatcttagaa catcagattc aagtaagacc
841 attcaacgca ttgaagacta agaatatgag aaacctgaat ccagaagaca ttgaccagct
901 catcaccatc agcggcatgg tgatcaggac atcccagctg attcccgaga tgcaggaggc
961 cttcttccag tgccaagtgt gtgcccacac gacccgggtg gagatggacc gcggccgcat
1021 tgcagagccc agtgtgtgcg ggcgctgcca caccacccac agcatggcac tcatccacaa
1081 ccgctccctc ttctctgaca agcagatgat caagcttcag gagtctccgg aagacatgcc
1141 tgcagggcag acaccacaca cagttatcct gtttgctcac aatgatctcg ttgacaaggt
1201 ccagcctggg gacagagtga atgttacagg catctatcga gctgtgccta ttcgagtcaa
1261 tccaagagtg agtaatgtga agtctgtcta caaaacccac attgatgtca ttcattatcg
1321 gaaaacggat gcaaaacgtc tgcatggcct tgatgaagaa gcagaacaga aacttttttc
1381 agagaaacgt gtggaattgc ttaaggaact ttccaggaaa ccagacattt atgagaggct
1441 tgcttcagcc ttggctccaa gcatttatga acatgaagat ataaagaagg gaattttgct
1501 tcagctcttt ggcgggacaa ggaaggattt tagtcacact ggaaggggca aatttcgggc
1561 tgagatcaac atcttgctgt gtggcgaccc tggtaccagc aagtcccagc tgctgcagta
1621 cgtgtacaac ctcgtcccca ggggccagta cacgtctggg aagggctcca gtgcagttgg
1681 cctcactgcg tacgtaatga aagaccctga gacaaggcag ctggtcctgc agacaggtgc
1741 tcttgtcctg agtgacaacg gcatctgctg tatcgatgag ttcgacagga tgaatgaaag
1801 tacaagatcg gtattgcatg aagtcatgga acagcagact ctgtccattg caaaggctgg
1861 gatcatctgt cagctcaatg cgcgcacctc tgtcctggca gcagcaaatc ccattgagtc
1921 tcagtggaat cctaaaaaaa caaccattga aaacatccag ctgcctcata ctttattatc
1981 aaggtttgat ttgatcttcc tcatgctgga ccctcaggac gaagcctatg acaggcgtct
2041 ggctcaccac ctggtcgcac tgtactacca gagcgaggag caggcagagg aggagctcct
2101 ggacatggcg gtgctaaagg actacattgc ctacgcgcac agcaccatca tgccgcggct
2161 aagtgaggaa gccagccagg ctctcatcga ggcttatgta gacatgagga agattggcag
2221 tagccgggga atggtttctg cataccctcg acagctagag tcattaatcc gcttagcaga
2281 agcccatgct aaagtaagat tgtctaacaa agttgaagcc attgatgtgg aagaggccaa
2341 acgcctccat cgggaagctc tgaagcagtc tgcaactgat ccccggactg gcatcgtgga
2401 catatctatt cttactacgg ggatgagtgc cacctctcgt aaacggaaag aagaattagc
2461 tgaagcattg aaaaagctta ttttatctaa gggcaaaaca ccagctctaa aataccagca
2521 actttttgaa gatattcggg gacaatctga catagcaatt actaaagata tgtttgaaga
2581 agcactgcgt gccctggcag atgatgattt cctgacagtg actgggaaga ccgtgcgctt
2641 gctctgaagc cttgtgagca aggaaggctc cctgcatgtc ctgcttgctg cacgccacat
2701 gggtgtggtc tgcatctcag ttggccgcca tcagtgtaaa tagagcttaa agtcatggtt
2761 tggctgcata aaaattttct aacttgggtt caatatttgt agtgaagtat ctgttttcat
2821 ttttttcacg ttataaataa aaatactatg ctggccgggc
//