LOCUS AK022815 2321 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12753 fis, clone NT2RP2001226.
ACCESSION AK022815
VERSION AK022815.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2321)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Sasaki,N.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2321
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2001226"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006089"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 355..1530
/codon_start=1
/protein_id="BAB14257.1"
/translation="MDQCVTVERELEKVLHKFSGYGQLCERGLEELIDYTGGLKHEIL
QSHGQDAELSGTLSLVLTQCCKRIKDTVQKLASDHKDIHSSVSRVGKAIDKNFDSDIS
SVGIDGCWQADSQRLLNEVMVEHFFRQGMLDVAEELCQESGLSVDPSQKEPFVELNRI
LEALKVRVLRPALEWAVSNRGMLIAQDSSLEFKLHRLYFISLLMGGTTNQREALQYAK
NFQPFALNHQKDIQVLMGSLVYLRQGIENSPYVHLLDANQWADICDIFTRDACALLGL
SVESPLSVSFSAGCVALPALINIKAVIEQRQCTGVWNQKDELPIEVDLGKKCWYHSIF
ACPILRQQTTDNNPPMKLVCGHIISRDALNKMFNGSKLKCPYCPMEQSPGDAKQIFF"
BASE COUNT 596 a 493 c 599 g 633 t
ORIGIN
1 gtgccgcccg ggagaaccag gtcatcggtc ggttcccgtg aaaacaaaaa caatcggccg
61 cgccgtcgca ggcacccgaa cgtcgcgagc ggggcctggg gacgcggagc cgagtgcagc
121 gagcgaacgg gagcagcggc gactcgccgg ggggctaggg cgccatgggg caggcgggct
181 ccggctgcgc ggggctcccc cggcgccgcg gctagtgcgc ccgccgcctc ggccgcctca
241 gcctcccgcg ccgcccgctt ggggaacgag gagcaggacg cggcctcggt ggggcccggg
301 ccgaacggct gcggacacct gggcgccgag gagccgagcg ccgccgtctc cggcatggat
361 cagtgcgtga cggtggagcg cgagctggag aaggtgctgc acaagttctc aggctacggg
421 cagctgtgcg agcgcggcct ggaggagctc atcgactaca ccggcggcct caagcacgag
481 atcctgcaga gccacggcca agatgctgaa ttatcaggga cactttcact tgttttgaca
541 cagtgctgta aaagaataaa ggatactgtt caaaaattgg cctccgacca caaagacatt
601 cacagcagtg tttctcgggt tggaaaagcc attgataaga attttgattc tgacattagc
661 agtgtgggaa tagatggctg ctggcaggca gacagccaaa ggcttctcaa tgaagtgatg
721 gtggagcact tctttcgaca aggaatgctg gatgtggctg aggagctctg tcaggaatct
781 ggtctttctg tagacccaag tcagaaggaa ccatttgtgg agttaaatag aatattagag
841 gcattaaagg tcagagttct gagacctgct ctggagtggg cagtgtcaaa ccggggaatg
901 cttatagctc aagacagctc cttggaattt aagctacaca gactgtattt tattagcttg
961 ttaatgggtg gaaccacaaa tcagcgagag gcattacaat atgctaaaaa ttttcagcca
1021 tttgccctaa atcatcaaaa agacattcag gttttgatgg gaagccttgt gtacctgaga
1081 caagggattg agaactcacc atatgttcac ctacttgatg caaaccagtg ggctgatatc
1141 tgtgacatct ttacacggga tgcttgtgcc ctcctggggc tctccgtgga gtcccctctc
1201 agtgtcagtt tctcagcagg ttgtgtggcg ctgccagctt taattaacat caaagccgtg
1261 attgaacaga ggcagtgtac tggagtttgg aaccagaaag atgaattacc tattgaagtg
1321 gaccttggta aaaagtgctg gtatcactct atatttgcct gccccattct tcgtcagcaa
1381 acaacagata acaatccacc catgaaattg gtctgtggtc atattatatc aagagatgcc
1441 ctgaataaaa tgtttaatgg tagcaaatta aaatgtccct actgtccaat ggaacaaagt
1501 ccaggagatg ccaaacagat atttttctga agagataact ttagtttgca atttgtaagt
1561 gaaattgaat cgtgggtgca tttcagaaga gaacgttcca tataatgcag ctaaccaagg
1621 actcctgtgt ttctataagc taatgctcca gaaactttgc caacctgtta gtgtacacac
1681 actgagggga gtgctcccgg tgaatattat catagggctt tattatattc ttggtcttca
1741 tttctgatca agtaaataca ccagcagttg tcattcaatg caggtttttg tacttaatta
1801 tatggtgatt tttttacttt ttaagagcag aaacggaaat tgacctcccc gccatgtgtc
1861 taatattcct cctgctttta cttttgtcat tttcttgata atcgtaagcc ttgagagtgt
1921 ttgtgaaaaa gttttatttc ctgttatgta tacataatta aatgaaaatt cttcagaaaa
1981 agtttgataa attgaattgt ggttatgaaa ctaatttgca tttttatttg cttaagaaag
2041 aaagctgtga tagattccag atatgctttt tgatgttttc ctctgctcca gctccaagaa
2101 gtcagcacac ctgcatttta gctctgcatg cagccccagc aggctgcgtg tttaagaatt
2161 tcattgttta actggctggt gtgagaagtc ttccgttagc atagagtgga aggagtacta
2221 ttgtttggtt gggtttttgt ttgtttgttt tttgtttttg cttttattgc caagaggtgc
2281 ttgttttaaa agtatgttta ataaaatgaa attctaaagt t
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