LOCUS AK022808 2687 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ12746 fis, clone NT2RP2000842, highly similar
to Lysophosphatidic acid receptor Edg-2.
ACCESSION AK022808
VERSION AK022808.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2687)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Sasaki,N.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2687
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2000842"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000006082"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 349..1443
/codon_start=1
/note="highly similar to Lysophosphatidic acid receptor
Edg-2"
/protein_id="BAG51118.1"
/translation="MAAISTSIPVISQPQFTAMNEPQCFYNESIAFFYNRSGKHLATE
WNTVSKLVMGLGITVCIFIMLANLLVMVAIYVNRRFHFPIYYLMANLAAADFFAGLAY
FYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLLAIAIERHITVFRMQLHTRMS
NRRVVVVIVVIWTMAIVMGAIPSVGWNCICDIENCSNMAPLYSDSYLVFWAIFNLVTF
VVMVVLYAHIFGYVRQRTMRMSRHSSGPRRNRDTMMSLLKTVVIVLGAFIICWTPGLV
LLLLDVCCPQCDVLAYEKFFLLLAEFNSAMNPIIYSYRDKEMSATFRQILCCQRSENP
TGPTEGSDRSASSLNHTILAGVHSNDHSVV"
BASE COUNT 715 a 617 c 572 g 783 t
ORIGIN
1 acggcgcgct gggctcacac tgtcccgccg cggacgggct ttgtggttgg gggcgcgcgt
61 gcgagtgcca gtgagagtgt gggtgcgcgc tgtgggccgc ggcgcgggtg ggtggccgtg
121 cgttcttgcg agccggcctg caggaggcga ggctcccctg gcctcccgca cccagcggcg
181 gaccgagccc ctggagggaa gttgccgcag ccgcccgggc cgccggccct cctgtcccgc
241 gccaggtaca cagcttctcc tagcatgact tcgatctgat cagcaaacaa gaaaatttgt
301 ctcccgtagt tctggggcgt gttcaccacc tacaaccaca gagctgtcat ggctgccatc
361 tctacttcca tccctgtaat ttcacagccc cagttcacag ccatgaatga accacagtgc
421 ttctacaacg agtccattgc cttcttttat aaccgaagtg gaaagcatct tgccacagaa
481 tggaacacag tcagcaagct ggtgatggga cttggaatca ctgtttgtat cttcatcatg
541 ttggccaacc tattggtcat ggtggcaatc tatgtcaacc gccgcttcca ttttcctatt
601 tattacctaa tggctaatct ggctgctgca gacttctttg ctgggttggc ctacttctat
661 ctcatgttca acacaggacc caatactcgg agactgactg ttagcacatg gctccttcgt
721 cagggcctca ttgacaccag cctgacggca tctgtggcca acttactggc tattgcaatc
781 gagaggcaca ttacggtttt ccgcatgcag ctccacacac ggatgagcaa ccggcgggta
841 gtggtggtca ttgtggtcat ctggactatg gccatcgtta tgggtgctat acccagtgtg
901 ggctggaact gtatctgtga tattgaaaat tgttccaaca tggcacccct ctacagtgac
961 tcttacttag tcttctgggc cattttcaac ttggtgacct ttgtggtaat ggtggttctc
1021 tatgctcaca tctttggcta tgttcgccag aggactatga gaatgtctcg gcatagttct
1081 ggaccccggc ggaatcggga taccatgatg agtcttctga agactgtggt cattgtgctt
1141 ggggccttta tcatctgctg gactcctgga ttggttttgt tacttctaga cgtgtgctgt
1201 ccacagtgcg acgtgctggc ctatgagaaa ttcttccttc tccttgctga attcaactct
1261 gccatgaacc ccatcattta ctcctaccgc gacaaagaaa tgagcgccac ctttaggcag
1321 atcctctgct gccagcgcag tgagaacccc accggcccca cagaaggctc agaccgctcg
1381 gcttcctccc tcaaccacac catcttggct ggagttcaca gcaatgacca ctctgtggtt
1441 tagaacggaa actgagatga ggaaccagcc gtcctctctt ggaggataaa cagcctcccc
1501 ctacccaatt gccagggcaa ggtggggtgt gagagaggag aaaagtcaac tcatgtactt
1561 aaacactaac caatgacagt atttgttcct ggaccccaca agacttgata tatattgaaa
1621 attagcttat gtgacaaccc tcatcttgat ccccatccct tctgaaagta ggaagttgga
1681 gctcttgcaa tggaattcaa gaacagactc tggagtgtcc atttagacta cactaactag
1741 acttttaaaa gattttgtgt ggtttggtgc aagtcagaat aaattctggc tagttgaatc
1801 cacaacttca tttatataca ggcttccctt ttttattttt aaaggatacg tttcacttaa
1861 taaacacgtt tatgcctatc agcatgtttg tgatggatga gactatggac tgcttttaaa
1921 ctaccataat tccatttttt cccttacata ggaaaactgt aagttggaat tatcttttgt
1981 ttagaaagca tgcatgtaat gtatgtatgc agtatgcctt acttaaaaag attaaaagga
2041 tactaatgtt aaatcttcta ggaaatagaa cctagacttc aaagccagta tttgtttagg
2101 tcatgaagca aacaatgctc taatcacaat attaactgtt taattaaaat gttgtaacaa
2161 gtataaaaca gggaatgtaa gtttattacc aaagtgatat gtattccaaa aaagtcatag
2221 aagatgaagc actataatat tgttcccata tatttaaaat acccaagtac attctaatta
2281 ccagtatatc agaggaaaat tttcgtagtc tttgtaaaat aatatactca tcatagaaaa
2341 cttgaaaaat gcagaaatgt ataaaaaagc aaaaatgatt actgataata tcacaaccca
2401 gaagtaacca cctttaaaaa gcaaccccca tgtatgccta tatgtgtatt gtatactttt
2461 tttacataat tggagtcata ctgtaaacag ttttataagt agatcttttt cattgcaaaa
2521 ttgccacatt ttcttatggc attaaaaatt ttacaaaaac ataattttaa tggctatatt
2581 atattccatt taatggatgc aactcagttt atttaaccat tcccatgttg ttaactattt
2641 aggttgtttc taattttcat tattataaag ttgcagaaat ttggtgt
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