LOCUS AK022751 2963 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12689 fis, clone NT2RM4002565, highly similar
to Mus musculus Sec8 mRNA.
ACCESSION AK022751
VERSION AK022751.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2963)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2963
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4002565"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000006025"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 10..1728
/codon_start=1
/protein_id="BAB14225.1"
/translation="MKNTRTASEPSAQLSYASTGREFAAFFAKKKPQRPKNSLFKFES
SSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGGGTKFVCKPGARNITVIFHPLL
RFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILA
NADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCT
AAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAA
FGKESEVLIGNLGDKLIPPQDILRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQM
LSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADRCLLVLHLEVRVHCFHYLIPLA
KEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYIFEGLGHLISCIL
INGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELL
NLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKD
KKITTV"
BASE COUNT 826 a 673 c 695 g 769 t
ORIGIN
1 tacttggata tgaaaaatac tcgtacggcc tctgaaccat cagctcaact aagctatgcc
61 agcactggac gagagtttgc agcctttttt gccaagaaga aacctcaaag gccaaaaaat
121 tctcttttca agttcgaatc gtcctcccat gccatcagta tgagcgccta tctgcgagaa
181 cagagaaggg agctctatag tcggagtgga gaactgcaag ggggtcctga tgacaactta
241 attgaaggtg gaggaacaaa atttgtctgc aaacctggag ccagaaacat taccgtcata
301 ttccacccat tactaagatt tattcaggag attgagcatg ctctgggtct tggcccagcc
361 aaacagtgtc ctcttcgaga gtttctcacc gtgtacatca aaaacatctt tctcaatcaa
421 gtcttggctg agatcaacaa ggagattgaa ggagtcacta aaacatctga ccctttgaag
481 attctggcca acgcagacac catgaaggtg ctgggagtgc agcggcctct cctacagagc
541 acaatcattg tggagaagac agttcaagac ctcctgaacc tgatgcatga cttgagtgca
601 tattcagatc aattcctcaa catggtgtgc gtgaagctcc aggagtacaa ggacacctgc
661 actgcagctt acaggggtat tgtccagtca gaagaaaaac ttgtcatcag tgcatcctgg
721 gcaaaagatg atgatatcag cagactcttg aaatctctac caaactggat gaatatggct
781 caacccaaac agctgaggcc aaaaagagag gaggaagaag atttcataag ggcagctttt
841 ggcaaggagt ctgaagttct tattgggaac ctgggtgata aattaatccc tccacaagac
901 atccttcgtg acgtcagtga cctcaaagcc ttggccaaca tgcatgaaag cctggaatgg
961 ttggcaagtc gaacaaagtc agctttctcc aatctttcta catcccagat gctttctcct
1021 gctcaagaca gccacacgaa cacggatctc cccccagtgt cagagcagat catgcagact
1081 ctcagtgaac ttgccaaatc gttccaggat atggctgacc gctgcttgct tgtcttacat
1141 ctggaagtga gggttcactg tttccactat cttatccctc ttgcaaagga ggggaactat
1201 gccattgtgg ctaatgtgga aagtatggat tatgaccccc tggtggtcaa gctcaacaaa
1261 gatatcagcg ccattgaaga ggccatgagc gccagccttc agcagcacaa gttccagtat
1321 atcttcgaag gcctgggcca cctgatctcc tgcatcctca ttaatggtgc ccagtacttc
1381 aggcgcatca gtgagtctgg catcaagaaa atgtgtagga acatttttgt tcttcagcag
1441 aatttgacca acatcaccat gtcgcgggag gcagacctgg actttgcaag gcagtactac
1501 gagatgcttt acaacacagc tgacgagctc ctgaacctgg tggtggacca gggtgtgaag
1561 tacacggagc tggagtacat ccacgctctg accctgctgc accgcagcca aactggggtg
1621 ggggaactga ccacccagaa cacgaggctg cagaggctca aagagatcat ctgcgagcag
1681 gctgccatca agcaagccac caaggacaag aagataacta ccgtttagca gggcgtactg
1741 cggttggtga cggtggtccc ctcagtcaca ctcacttttt tccttggtat gttattgagt
1801 atattctgag cttagttttc tctacagtga tactttagtg gagaggaggt gtaaggattc
1861 tttctctctg gttttggctt ttcatataaa tgctaaagga aggcgacagt acagagtgtt
1921 ttggttgaac aactacttta atgcagtcaa atctaacggg actagggtgg gatagggagg
1981 aaggtggtat caaattgttg gactctgaaa aacaagtgga tggattacta atatttgatt
2041 tattactcaa tatatatata attccagcct tggaaagtag gaaagtaggc tgctacctaa
2101 cttttagtcc cttccaaaac catttagtag ggttctatta cataaacagt agggtttttc
2161 ctctcctgac cttatccgtt tacagttcaa tgaaataagt gtatgtgcat tgctgtctgt
2221 ctctattgcc aaaatcaaat aaatggcttc tcagctgctg tttacaacaa acttccctaa
2281 ccaaaacaga acctctaatt tagggctagg aatagaagtc ttttgatccc agtccgtggg
2341 acagttttgt atgctgtatc ttgtacacag gttgtaggtt ggtttattgc cattttgttt
2401 tatttctgct atataaaaac caagagccag acaattagac aactatggag gaatgaaagg
2461 caattcaaac atctgcttga ttcccgcccc gccaccccta atttctgaga gaataatggt
2521 tagagaacat tgcatctcac ttgaaacatc ctttgggggt aaatgattct taggtgctca
2581 cttggtctga gctcagctga atgcagggaa gatgggacag agtgacattt ctcctctgtg
2641 cggcctccag ctgagcctgt ctctaaaaag aattgttccc tcccccgcaa tgtgggcact
2701 taccatgttc ctggcacact ggacctcacc atgtcccccg gtcaccagtc ctcttgctgc
2761 ccatggttca taggtcatag aaggcagcaa tgcactgact gggccacatc ttgaaaactc
2821 gtggaaaggg aaaggggagg agatgggact ttgaccacac acagtgatgc ctcacaccct
2881 gaaggtgggg gattttgtac ctgtttgtac aactttggtc ttgatgggaa aaatggagaa
2941 ttaaaaagtg ttttgagaag ctt
//