LOCUS AK022750 2511 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12688 fis, clone NT2RM4002534.
ACCESSION AK022750
VERSION AK022750.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2511)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2511
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4002534"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000006024"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 90..1961
/codon_start=1
/protein_id="BAB14224.1"
/translation="MDSLLPVLGVLFLLYCFTKQSVSHIRSLCQEILLWILGKLERKK
AIASLKGFAGLDKAVPSLHSLCQFRVATQGGIMITIKEAISDEDEDEALYQKVSSEQG
RVEHLGDLLSHCQECGLAGDFFIFCLKELTHVASENETELKTEPFSSKSLLELEQHQT
LLVEGQERKLLVLQLMAVLCERMSEQIFTNVTQVVDFVAATLQRACASLAHQAESTVE
SQTLSMSMGLVAVMLGGAVQLKSSDFAVLKQLLPLLEKVSNTYPDPVIQELAVDLRIT
ISTHGAFATEAVSMAAQSTLNRKDLEGKIEEQQQTSHERPTDVAHSHLEQQQSHETAP
QTGLQSNAPIIPQGVNEPSTTTSQKSGSVTTEQLQEVLLSAYDPQIPTRAAALRTLSH
WIEQREAKALEMQEKLLKIFLENLEHEDTFVYLSAIQGVALLSDVYPEKILPDLLAQY
DSSKDKHTPETRMKVGEVLMRIVRALGDMVSKYREPLIHTFLRGVRDPDGAHRASSLA
NLGELCQRLDFLLGSVVHEVTACLIAVAKTDGEVQVRRAAIHVVVLLLRGLSQKATEV
LSAVLKDLYHLLKHVVCLEPDDVAKLHAQLALEELDDIMKNFLFPPQKLEKKIMVLP"
BASE COUNT 652 a 579 c 675 g 605 t
ORIGIN
1 ctattttggt gacagaagaa gaacttagta gatgcattga ggatgtgttt aaggtgtacg
61 tggttgggaa tgaaccttta acagttttga tggattccct gcttccagtc ctgggagtgc
121 tttttcttct ctactgtttt actaagcaga gtgtgtctca cataaggtca ctttgccaag
181 aaatcttatt atggattctg gggaagctgg aaaggaagaa ggcaattgcc agcctgaaag
241 gatttgcagg gttggacaaa gctgtgccct ctctccattc tctgtgtcag tttagagttg
301 ccactcaagg tggcattatg attaccatca aagaggccat tagtgatgaa gatgaagatg
361 aagccctgta ccagaaggta tcctctgagc agggccgggt ggagcatctc ggggacttgc
421 tgtcccactg ccaggaatgc ggtttggcag gagacttctt catcttctgt ttgaaagagt
481 tgactcatgt ggcctcggaa aatgaaacag agttaaaaac tgagcccttc tccagcaaga
541 gcctcttgga attagagcaa catcagactc ttcttgtgga aggccaagag cggaagctgc
601 ttgtcctgca gctgatggct gttctgtgcg agagaatgtc tgagcagata ttcacaaacg
661 tcactcaggt ggtggacttt gtagcagcaa cattgcagag agcctgtgca agcctggccc
721 atcaggcaga gagcaccgtg gaatcacaga cgctgagcat gtccatgggg ctggtggctg
781 tcatgctagg aggagctgtt cagttgaagt caagtgattt tgctgttctg aagcagttgt
841 tgcctctgtt ggagaaggta tccaacacat accctgatcc ggtcatccaa gaactcgctg
901 ttgatctccg catcaccatc tctacccatg gagcctttgc cactgaggcc gtcagcatgg
961 ctgcccaaag tacactgaac agaaaagatc tggaagggaa aatagaagag cagcaacaaa
1021 ccagtcatga aagacccact gatgtagctc atagccacct tgaacaacag cagagccatg
1081 agacagcccc ccagacaggc ctgcagtcaa atgctccaat cattcctcaa ggagtcaatg
1141 agcccagcac tactacaagt cagaaatctg gaagcgtaac cacagaacag ctccaagagg
1201 ttcttttgtc agcttatgac cctcaaattc caacacgggc tgctgccctg cgtactcttt
1261 cccactggat agagcagaga gaagcaaaag cccttgagat gcaagagaag cttctcaaga
1321 tattcttgga aaacttggaa catgaagaca cttttgtata tctatctgca attcaggggg
1381 ttgccctgct gtcagacgtc tatcctgaga aaatcttgcc ggacttgttg gctcaatatg
1441 acagcagcaa agacaagcac acaccagaga ccagaatgaa agtcggggaa gtccttatgc
1501 gaatcgtcag ggcattagga gacatggtct caaagtaccg agaacctttg atccatacct
1561 tcctgagggg agtgagagat cctgatggtg ctcacagggc cagcagcttg gccaaccttg
1621 gggagctgtg ccagaggctg gactttctgc tgggctccgt ggtccatgag gtaacagctt
1681 gcctgattgc tgtggccaaa acagatggtg aagttcaagt acgcagagct gccatacatg
1741 tggttgtgct gctgcttcgg ggactcagcc agaaagctac tgaggtgctg agcgccgtcc
1801 tcaaggatct ctaccacctg ctgaagcacg tagtgtgtct ggagcccgat gacgtggcca
1861 agctccatgc ccagttggcc ctagaagagc tggatgacat catgaaaaac ttcctgttcc
1921 ctccacagaa gctggagaag aagatcatgg tcctgccgta gacctggctc caaggacgtg
1981 gaggaggcag gcagggccag gcacccagag ccgtgcccag gtcttccagc aggtggccct
2041 gctgcctctt gagtgctggc agcatggctg accctcgggg tggttttatg gtgcaggtca
2101 cttgggtctt cagggtccct tccgagggca tgtgttcagc actcccgcgt tcagcctgag
2161 gggtgtacag ttaagagaag acagttacag atctcattaa tctacatttt tcactgtcct
2221 ctagcattga aagaaggatg tctacctggt gaaagtatat tttaacatga ctgatggaat
2281 ttcactaatt gcccactctc ttggaacttg aggagaagcg gttggccacc catatgtcac
2341 ctagctctat attctttcag gctgagattc ttcttcagga aaatgaggag cagaactggc
2401 cacccttggc tgctcaagag gcatttcaga gtaggagatg cagttggagg tggggagggg
2461 aaggaaatag ttatcaaata atgcaaaatg gaataaagtt atcttggatg g
//