LOCUS AK022623 2603 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ12561 fis, clone NT2RM4000798, highly similar
to Brefeldin A-inhibited guanine nucleotide-exchange protein 2.
ACCESSION AK022623
VERSION AK022623.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2603)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2603
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4000798"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000005897"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 80..2332
/codon_start=1
/note="highly similar to Brefeldin A-inhibited guanine
nucleotide-exchange protein 2"
/protein_id="BAG51099.1"
/translation="MGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGS
TRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRPQWSRIWHV
IGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNR
SPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVT
TIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEY
TSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKH
WWQDLFRIVLRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSD
VFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHV
LLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSSPTDDSWKGRPYANQ
KLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQG
MYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSL
ACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLL
TKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPS
QVPAALSPVW"
BASE COUNT 693 a 589 c 638 g 683 t
ORIGIN
1 gaagacgcgc tacctgtctg gatctgggcg tgaaagagaa gggagcctga agggccacac
61 attggcagga gaagagttca tgggccttgg cctcggtaat ttggtgagtg gcggagtgga
121 taaaagacag atggccagct tccaagaatc ggttggtgag accagctcgc agagtgtggt
181 tgtagctgtg gacaggattt ttactgggtc taccagactg gatggaaatg caatagttga
241 ctttgtccgc tggctgtgtg ctgtgtccat ggatgaactg gcttcccccc accatcctcg
301 catgttcagc ttgcagaaga ttgtggagat atcatactac aacatgaatc ggatccgacc
361 acagtggtct cgaatatggc atgtgattgg agatcacttc aataaggttg gctgcaaccc
421 taatgaagat gtggctatct ttgctgttga ctcattaagg caactctcca tgaagtttct
481 tgagaagggt gaattagcca acttccgttt ccagaaagat tttctgaggc cctttgagca
541 tattatgaag aaaaacaggt ctcccaccat ccgggacatg gcgatccgct gcattgccca
601 gatggtgaac tcccaggcgg ccaacatccg ctcaggttgg aagaacatct ttgccgtgtt
661 ccaccaggca gcctctgatc atgatgggaa cattgtggag ctggccttcc agaccacttg
721 ccacattgtc acaactattt tccagcacca ttttcctgca gccatcgatt cctttcagga
781 tgctgtgaag tgcttatcag agttcgcctg caacgccgct ttccctgaca cgagcatgga
841 agcgattcgg ctcatccgct tctgtggcaa atacgtctct gagaggcctc gggtgctaca
901 agaatacaca agtgatgaca tgaatgtagc tcctggtgac agagtctggg tccgaggctg
961 gttccccatc ttattcgaac tctcctgcat cattaataga tgcaagttag atgtacgaac
1021 aaggggactc acagtcatgt ttgagatcat gaagagctat ggccacacct ttgaaaagca
1081 ctggtggcag gacctgttca gaatcgtgct tcggattttt gacaatatga aactccctga
1141 gcaactgtca gagaaatctg agtggatgac aacaacctgc aatcacgcac tttatgctat
1201 ttgtgatgtt tttacccagt tttatgaagc tttgaatgaa gttcttcttt ctgatgtatt
1261 tgcacaattg cagtggtgtg tcaaacaaga taatgaacag ttggcgcgat caggtacaaa
1321 ttgcttagaa aacttagtaa tatccaatgg agagaaattc agtcctgaag tctgggatga
1381 aacctgcaac tgtatgttgg atattttcaa aacaaccatc ccacatgttt tgctgacatg
1441 gagacctgta ggaatggagg aagattcatc agaaaagcat ttggatgtgg atctggaccg
1501 ccagtcttta agcagcatag ataaaaatcc ctctgagagg ggacagagcc agctctctag
1561 cccaacagat gacagctgga agggtagacc atacgcaaat cagaaactgt ttgccagcct
1621 cctcatcaag tgtgtggtcc agttggaatt gatacagacc attgacaaca ttgtgttcta
1681 ccctgcgacg agcaaaaagg aggatgcaga gcacatggtt gccgcccagc aagacacgct
1741 ggatgcagat atccacatag agacggagga tcagggcatg tataagtaca tgtcttccca
1801 gcacctcttc aagctgttgg actgtttgca ggaatcccat tcattctcaa aggccttcaa
1861 ctccaattac gagcagcgga ctgtcctgtg gcgagcaggt tttaagggca agtctaaacc
1921 caatcttcta aaacaagaaa ccagcagcct ggcctgttgt ttgaggatcc tgtttcgaat
1981 gtatgttgat gagaaccgca gggattcctg ggaagaaata cagcagagac ttttaactgt
2041 ttgcagtgaa gctcttgcct atttcatcac tgtgaattct gagagccatc gggaggcctg
2101 gacaagtctc ttgttgttac ttctaactaa aaccctcaaa ataaatgatg aaaagttcaa
2161 agcacatgct tcaatgtact acccctactt gtgtgaaatt atgcagtttg acctgatccc
2221 tgagctccga gcagttctgc ggaagttctt cctacggata ggtgttgtgt ataagatatg
2281 gataccagaa gagccatcac aggtaccagc agcactgtca ccagtgtggt agccctggct
2341 gcccaggcca gtgctgcagc tctgcagaat gttcagcatg ccatttctga ctggcacatc
2401 ccgtgaagtt tcatagaaac aaggagttgg catcttggat ctcagaatgg cctggaaacg
2461 gatggcctct acgctgttcc atcacagtct ccaactaagg cttatggtat ttcattaaac
2521 tgttgcatac ccagttagca cagtaggtgg ggagtctgct tcatttctat cattccattt
2581 ttctgattaa actgtcaaat ctg
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