LOCUS AK022579 2550 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12517 fis, clone NT2RM2001784.
ACCESSION AK022579
VERSION AK022579.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2550)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Sasaki,N.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2550
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM2001784"
/clone_lib="NT2RM2"
/db_xref="H-InvDB:HIT000005853"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 1..1392
/codon_start=1
/protein_id="BAB14109.1"
/translation="MRAGPEPQALVGQKRGALRLLVPRLVLTVSAPAEVRRRVLRPVL
SWMDRETRALADSHFRGLGVDVPGVGQAPGRVAFVSEPGAFSYADFVRGFLLPNLPCV
FSSAFTQGWGSRRRWVTPAGRPDFDHLLRTYGDVVVPVANCGVQEYNSNPKEHMTLRD
YITYWKEYIQAGYSSPRGCLYLKDWHLCRDFPVEDVFTLPVYFSSDWLNEFWDALDVD
DYRFVYAGPAGSWSPFHADIFRSFSWSVNVCGRKKWLLFPPGQEEALRDRHGNLPYDV
TSPALCDTHLHPRNQLAGPPLEITQEAGEMVFVPSGWHHQVHNLDDTISINHNWVNGF
NLANMWRFLQQELCAVQEEVSEWRDSMPDWHHHCQVIMRSCSGINFEEFYHFLKVIAE
KRLLVLREAAAEDGAGLGFEQAAFDVGRITEVLASLVAHPDFQRVDTSAFSPQPKELL
QQLREAVDAAAAP"
BASE COUNT 431 a 774 c 819 g 526 t
ORIGIN
1 atgcgtgcag gcccggagcc ccaggcgctg gtggggcaga aacgcggcgc cctgcgtctt
61 ctggttccga ggctggtcct caccgtttcc gctccggcgg aagtgaggag gagggtcctt
121 cgacccgtgc tgagctggat ggaccgcgag acgcgcgccc tcgccgacag ccacttccga
181 ggcctggggg tcgatgtccc cggcgtcggc caggctccgg gccgggtagc cttcgtctcg
241 gagccgggcg ccttctccta cgccgacttt gtgcggggct tcttgctgcc caacctgccc
301 tgcgtgtttt ccagcgcctt cacgcagggc tggggcagcc ggcggcgctg ggtgacgccc
361 gcggggaggc ccgacttcga ccacctgcta cggacctacg gagacgtggt tgtaccagtt
421 gcaaactgtg gggtccagga atacaactcg aaccccaaag agcacatgac tctcagagac
481 tacatcacct actggaaaga gtacatacag gcgggctact cctctcccag gggctgtctc
541 tacctcaaag actggcactt gtgcagggac tttccggtgg aggacgtttt caccctgcct
601 gtgtacttct cgtccgactg gctgaatgag ttctgggatg cactggatgt ggatgactac
661 cgctttgtct acgcggggcc tgcgggcagc tggtccccgt tccatgctga catcttccgc
721 tccttcagct ggtctgtcaa tgtctgtggg aggaagaagt ggctcctctt ccccccaggg
781 caggaagagg ccctgcggga ccgccacggc aacctgccct acgacgtgac ctccccagca
841 ctctgcgaca cacacctgca cccacggaac cagcttgctg gcccaccctt ggagatcacg
901 caggaagcgg gcgagatggt gtttgtgccc agtggctggc accaccaggt gcacaacctg
961 gatgacacca tctccatcaa ccacaactgg gtcaatggct tcaacctggc caacatgtgg
1021 cgcttcttgc agcaggagct atgcgccgtg caggaggagg tcagcgagtg gagggactcc
1081 atgcccgact ggcaccacca ctgccaggtc atcatgaggt cctgctcggg catcaacttt
1141 gaagagtttt accacttcct caaggtcatc gctgagaaga ggctcctggt cctgagggag
1201 gcagccgctg aggacggtgc tgggttgggt ttcgaacagg cagcctttga tgttgggcgc
1261 atcacagagg tgctggcctc cttggttgcg caccccgact tccagagagt ggacaccagc
1321 gcgttctcac cacagcccaa agagctgctg cagcagctga gagaggctgt tgatgctgct
1381 gcggccccat agcacctgtc gtgaggatag aaggacgggt ggaagagagg cagcctcctg
1441 ctccggggcc cttccagaaa taaagaccgc cctccctgtg acctggggcc cacccctgtc
1501 gaggcttgtg gcctggctgt tcatggccac tgcctgggtg cctgttttca ggtgaggccc
1561 aatgaggtca gggacccaag atgggatgtg gcccttctga cctgcagcag gcctgctggg
1621 agctcggaga tggtgccagg acctggctct tttgggggcc ctgcctcctt aggccaggac
1681 gcctgagctg acaggagtct gtgtctggtg tgccttctct ggtggctcct cttaataggc
1741 cagccctgtc ccctcgtctc aggccattgg accacccctg gctctgccct gtgggttcag
1801 ggaggggttg gagcagtgct gggcaagctc accagggcct ccaggcaggg ctggggttgg
1861 cctccatcac ctccaggtga tgggctgtgg aaccagcggc ctgcgccttc ctctgggtac
1921 ccagagtgga gggctgggtt gggctggcct ttgccacctc cctgcctttg cagggcctgt
1981 ggacggctgg agaggccaca gatggggtgg aatcccatct gctgctgaat cctcacctgg
2041 gcctgaggga ctgtgcctgc tgtgcactca cagctgggtc ttcccaagga tgctgttctc
2101 aggagtggtg ggtccccagc ccctcttcac actgggtatg atggaggtgt gggcgggctc
2161 gtccaggccg atcaaggcac agcagtgagc agcggaggcc tgtggtgggg gatggactct
2221 cgtgggatcc tcttgcagag gatgccccag gcctgaaccc tctagtggat ccacagtttg
2281 tggagactgg cactctccca gccctgtcct tgaccgagag tccagcattt tttcagttgg
2341 cccctggttg gctgcctcac cccagcaggg gaggaggcat ccgaatccac agggacggca
2401 cgtgccatgg ctatgtacat tgcctgcccg tggcatcaac tggggccgct ggcacttgtc
2461 taggatggaa gcccccaaga agggccgggg tttctgtctg ctctgttcag tgaatcatgt
2521 gaagtgcttg caaaggcagc tttacacagt
//