LOCUS AK022573 2892 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ12511 fis, clone NT2RM2001727, highly similar
to Homo sapiens ubiquitin protein ligase E3 component n-recognin 4
(UBR4), mRNA.
ACCESSION AK022573
VERSION AK022573.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2892)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ishii,S., Kawai,Y., Saito,K., Yamamoto,J.,
Wakamatsu,A., Nakamura,Y., Nagahari,K., Masuho,Y. and Sasaki,N.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2892
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM2001727"
/clone_lib="NT2RM2"
/db_xref="H-InvDB:HIT000005847"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 125..2578
/codon_start=1
/note="highly similar to Homo sapiens ubiquitin protein
ligase E3 component n-recognin 4 (UBR4), mRNA"
/protein_id="BAG51094.1"
/translation="MPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLV
NNKIISLDLPVAEVYKKVWCTTNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEED
EEEVYKMAGVMAQCGGLECMLNRLAGIRDFKQGRHLLTVLLKLFSYCVKVKVNRQQLV
KLEMNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGN
LLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYC
NFDKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIP
SAKNLDADIWKKFLSRPALPFILRLLRGLAIQHPGTQVLIGTDSIPNLHKLEQVSSDE
GIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQ
VVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEELENKPRKQ
QGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAF
ATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLI
PYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWVESAFEVDGPYYFTVLALHILPP
EQWRATRVEILRRLLVTSQARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNM
FKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDVAGL
LSEITDPESFLKDLLNSVP"
BASE COUNT 721 a 756 c 785 g 630 t
ORIGIN
1 ccggaccccg gtgttcatct tcgagaggct ctgcagcatc atttatcctg aggagaatga
61 agtcactgag ttctttgtga ccctggagaa ggatccccaa caagaagact tcttacaggg
121 caggatgcct gggaacccgt atagcagcaa tgagccaggc atcgggccgc tgatgaggga
181 tataaagaac aagatttgcc aggactgtga cttagtggcc ctcctggaag atgacagtgg
241 catggagctt ctagtgaaca ataaaatcat tagtttggac cttcctgtgg ctgaagttta
301 caagaaagtc tggtgtacca cgaatgaggg agagcccatg aggattgttt atcgtatgcg
361 ggggctgctg ggcgatgcca cagaggagtt cattgagtcc ctggactcta ctacagatga
421 agaagaagat gaagaagaag tgtataaaat ggctggtgtg atggcccagt gtgggggcct
481 ggaatgcatg cttaacagac tcgcagggat cagagatttc aagcagggac gccaccttct
541 aacagtgcta ctgaaattgt tcagttactg cgtgaaggtg aaagtcaacc ggcagcaact
601 ggtcaaactg gaaatgaaca ccttgaacgt catgctgggg accctaaacc tggcccttgt
661 agctgaacaa gaaagcaagg acagtggggg tgcagctgtg gctgagcagg tgcttagcat
721 catggagatc attctagatg agtccaatgc tgagcccctg agtgaggaca agggcaacct
781 cctcctgaca ggtgacaagg atcaactggt gatgctcttg gaccagatca acagcacctt
841 tgttcgctcc aaccccagtg tgctccaggg cctgcttcgc atcatcccgt acctttcctt
901 tggagaggtg gagaaaatgc agatcttggt ggagcgattc aaaccatact gcaactttga
961 taaatatgat gaagatcaca gtggtgatga taaagtcttc ctggactgct tctgtaaaat
1021 agctgctggc atcaagaaca acagcaatgg gcaccagctg aaggatctga ttctccagaa
1081 ggggatcacc cagaatgcac ttgactacat gaaaaagcac atccctagcg ccaagaattt
1141 ggatgccgac atctggaaaa agtttttgtc tcgcccagcc ttgccattta tcctaaggct
1201 gcttcggggc ctggccatcc agcaccctgg cacccaggtt ctgattggaa ctgattccat
1261 cccgaacctg cataagctgg agcaggtgtc cagtgatgag ggcattggga ccttggcaga
1321 gaacctgctg gaagccctgc gggaacaccc tgacgtaaac aagaagattg acgcagcccg
1381 cagggagacc cgggcagaga agaaacgcat ggccatggca atgaggcaga aggccctggg
1441 caccctgggc atgacgacaa atgaaaaggg ccaggtcgtg accaagacag cactcctgaa
1501 gcagatggaa gagctgatcg aggagcctgg cctcacgtgc tgcatctgca gggagggata
1561 caagttccag cccacaaagg tcctgggcat ttataccttc acgaagcggg tagccttgga
1621 ggagttggag aataagcccc ggaaacagca gggctacagc accgtgtccc acttcaacat
1681 tgtgcactac gactgccatc tggctgccgt caggttggct cgaggccggg aagagtggga
1741 gagtgccgcc ctgcagaatg ccaacaccaa gtgcaacggg ctccttccgg tctggggacc
1801 tcatgtccct gaatcagctt ttgccacttg cttggcaaga cacaacactt acctccagga
1861 atgtacaggc cagcgggagc ccacgtatca gctcaacatc cacgacatca aactgctctt
1921 cctgcgcttc gccatggagc agtcgttcag cgcagacact ggcgggggcg gccgggagag
1981 caacatccac ctgatcccgt acatcattca cactgtgctt tacgtcctga acacaacccg
2041 agcaacttcc cgagaagaga agaacctcca aggctttctg gaacagccca aggagaagtg
2101 ggtggagagt gcctttgaag tggacgggcc ctactatttc acagtcttgg cccttcacat
2161 cctgccccct gagcagtgga gagccacacg tgtggaaatc ttgcggaggc tgttggtgac
2221 ctcgcaggct cgggcagtgg ctccaggtgg agccaccagg ctgacagata aggcagtgaa
2281 ggactattcc gcttaccgtt cttcccttct cttttgggcc ctcgtcgatc tcatttacaa
2341 catgtttaag aaggtgccta ccagtaacac agagggaggc tggtcctgct ctctcgctga
2401 gtacatccgc cacaacgaca tgcccatcta cgaagctgcc gacaaagccc tgaaaacctt
2461 ccaggaggag ttcatgccag tggagacctt ctcagagttc ctcgatgtgg ccggtctttt
2521 atcagaaatc accgatccag agagcttcct gaaggacctg ttgaactcag tcccctgacc
2581 accacacagc agctgcggcg gcgaagacga agctggcttg ccttccacct tctgttctcc
2641 ctccttgtgc attaagttcc ctccgcggga tgctgcattg ttaccccgcc ctcccctctc
2701 tcatttttct tggtgtggct tggggttttt aggcttcctg ttttatctcg tgtgtgtggt
2761 gcaccagcta tgaggttgtc tgtaacccaa gccatcaaag ggcctgtaca tacctaggag
2821 ccatgagttg tcccggccag cttcatactt gagtgtgcac atcttgagaa ataaacaagt
2881 gacttaacac ac
//