LOCUS AK022509 3089 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ12447 fis, clone NT2RM1000271.
ACCESSION AK022509
VERSION AK022509.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3089)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..3089
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM1000271"
/clone_lib="NT2RM1"
/db_xref="H-InvDB:HIT000005783"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pUC19FL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 84..2972
/codon_start=1
/protein_id="BAB14067.1"
/translation="MASNSTKSFLADAGYGEQELDANSALMELDKGLRSGKLGEQCEA
VVRFPRLFQKYPFPILINSAFLKLADVFRVGNNFLRLCVLKVTQQSEKHLEKILNVDE
FVKRIFSVIHSNDPVARAITLRMLGSLASIIPERKNAHHSIRQSLDSHDNVEVEAAVF
AAANFSAQSKDFAVGICNKISEMIQGLATPVDLKLKLIPILQHMHHDAILASSARQLL
QQLVTSYPSTKMVIVSLHTFTLLAASSLVDTPKQIQLLLQYLKNDPRKAVKRLAIQDL
KLLANKTPHTWSRENIQALCECALQTPYDSLKLGMLSVLSTLSGTIAIKHYFSIVPGN
VSSSPRSSDLVKLAQECCYHNNRGIAAHGVRVLTNITVSCQEKDLLALEQDAVFGLES
LLVLCSQDDSPGAQATLKIALNCMVKLAKGRPHLSQSVVETLLTQLHSAQDAARILMC
HCLAAIAMQLPVLGDGMLGDLMELYKVIGRSATDKQQELLVSLATVIFVASQKALSVE
SKAVIKQQLESVSNGWTVYRIARQASRMGNHDMAKELYQSLLTQVASEHFYFWLNSLK
EFSHAEQCLTGLQEENYSSALSCIAESLKFYHKGIASLTAASTPLNPLSFQCEFVKLR
IDLLQAFSQLICTCNSLKTSPPPAIATTIAMTLGNDLQRCGRISNQMKQSMEEFRSLA
SRYGDLYQASFDADSATLRNVELQQQSCLLISHAIEALILDPESASFQEYGSTGTAHA
DSEYERRMMSVYNHVLEEVESLNRKYTPVSYMHTACLCNAIIALLKVPLSFQRYFFQK
LQSTSIKLALSPSPRNPAEPIAVQNNQQLALKVEGVVQHGSKPGLFRKIQSVCLNVSS
TLQSKSGQDYKIPIDNMTNEMEQRVEPHNDYFSTQFLLNFAILGTHNITVESSVKDAN
GIVWKTGPRTTIFVKSLEDPYSQQIRLQQQQAQQPLQQQQQRNAYTRF"
BASE COUNT 888 a 660 c 673 g 868 t
ORIGIN
1 aagaaggcgg cgggggaaga tggcggtcct ggggtagagt ttgcaagctt tctgactagg
61 ctagtcgagt aactattcgg gtcatggcgt caaactcaac taagtctttc ctggcagatg
121 ccggctatgg cgaacaggaa ctggatgcca actctgccct tatggaattg gacaaaggcc
181 taagatctgg caaacttggt gaacagtgtg aagcagttgt tcgctttccc agactttttc
241 agaagtatcc attccctatt cttatcaatt ctgcattcct aaagttagct gatgttttca
301 gagttggaaa taatttcctg aggctatgtg ttcttaaagt tacccaacaa agtgagaaac
361 atttggagaa gattctaaat gtggatgaat ttgtgaagag aattttttct gtgattcata
421 gtaatgatcc tgtggcaaga gccatcaccc tccggatgtt gggaagtctg gcatcaataa
481 ttcctgagag gaagaatgct catcatagta ttcgtcagag tttagattca catgataatg
541 tagaagttga agctgctgtt tttgctgctg caaacttctc tgcacagtca aaggattttg
601 ctgtaggaat ctgtaacaaa atcagtgaaa tgattcaagg tttagcgaca ccagtagact
661 tgaagctaaa attgataccc attctacagc acatgcacca tgatgcaatc ttggcttcca
721 gtgctcgtca gcttttacaa cagctggtca catcctatcc gtccaccaaa atggtgattg
781 tgtctttgca cactttcact ctgcttgcag cgtcatcttt ggttgataca cctaagcaga
841 ttcagcttct gttgcagtat ttgaagaatg atcccaggaa ggcagtaaag agacttgcta
901 ttcaagatct gaaattactt gctaataaaa caccacatac ttggagtagg gagaatattc
961 aggcactttg tgagtgtgcc ctccagactc cttatgacag cttaaaacta gggatgttgt
1021 ctgtcctttc cacactatca gggaccatcg ccatcaaaca ttacttcagt atagttccag
1081 gaaatgtgag ttcttctccc agatcttctg atttagtcaa attagcccaa gagtgctgtt
1141 accataataa caggggcatt gcagctcatg gagttagagt cctaactaat ataactgttt
1201 cttgtcaaga aaaggatctt ttggcactgg aacaagatgc tgtctttggc ctggaatccc
1261 tactggtact ttgtagtcaa gatgatagtc caggtgctca ggccacttta aagattgctc
1321 taaactgtat ggtgaagttg gccaagggca ggccccatct tagccagtca gtagttgaga
1381 ccttgttgac tcaattgcac agtgctcaag acgctgcccg gattttgatg tgccactgcc
1441 tggcagccat tgccatgcaa ctgccggtgc tgggtgatgg gatgcttggt gacctcatgg
1501 agctgtacaa ggtgattgga cgatcagcca cagacaagca acaagaactt ctggtgagtt
1561 tggctactgt gatttttgtt gcaagtcaga aggcattgtc tgtggaaagt aaggcagtaa
1621 ttaagcagca gcttgaaagt gtctccaatg gatggactgt ataccgtatt gccagacagg
1681 cttccagaat gggtaatcat gacatggcca aagagcttta tcagagtttg ctgactcagg
1741 ttgcctcaga acatttctac ttctggctaa atagtttgaa ggagttttca catgcagaac
1801 agtgtctcac tgggttgcaa gaggaaaatt atagttcagc actttcttgc attgctgaat
1861 ctttaaaatt ctatcacaaa gggattgctt ccttaacagc agctagtaca ccactgaatc
1921 ctttaagctt tcagtgtgaa tttgtaaaac tcaggattga ccttttacaa gccttctctc
1981 aacttatctg tacttgtaat agcctgaaga caagcccacc acctgcaatt gccacaacaa
2041 ttgccatgac cttaggaaat gacctccaga ggtgtggtcg catctccaat cagatgaaac
2101 agtccatgga agaatttcga agccttgctt ctcgatatgg agatctttac caggcatctt
2161 ttgatgctga ctcagcaact ttgaggaatg ttgaactaca gcagcagagc tgtttactga
2221 tatctcatgc aatagaagcc ctgattttgg atccagaatc agcaagtttc caggaatatg
2281 gatctactgg aacagcccat gctgatagtg aatatgaaag aagaatgatg tctgtatata
2341 atcatgtctt ggaggaggta gaatcactca atcggaaata tacccctgtt tcttatatgc
2401 acacagcatg cctctgcaat gccatcattg ctttgctgaa agttcccctt tctttccaga
2461 gatatttttt ccagaaacta cagtctacca gcatcaagct tgctctgtca ccatcgcccc
2521 ggaatcctgc agagcccatt gctgtccaga ataaccagca gctggcgcta aaggtagagg
2581 gagtggttca gcacggatct aaaccaggac tcttccgcaa aattcagtct gtctgtctga
2641 atgtttcttc cacactgcag agtaaatctg gacaagacta caagataccc attgacaaca
2701 tgaccaatga gatggagcaa agggttgaac ctcataatga ttacttcagt actcaatttc
2761 tgttgaactt tgctatcctt ggaacacaca acattacagt ggaatcttct gtgaaagatg
2821 ccaatggtat agtatggaag actggtccca gaactaccat atttgtaaaa tccctggaag
2881 acccttattc ccagcaaatt cgcttacaac agcagcaagc ccagcagcca ttacagcagc
2941 agcagcaacg caatgcctac acacggtttt aaccatggaa tgaatgcact gcagactctc
3001 aagagatcaa tcaaattgcc agaaacagtt tggtttttca tatggaataa gtattaaagt
3061 tacagtgtag ttcaaaaaaa aaaaaaata
//