LOCUS AK001584 2802 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ10722 fis, clone NT2RP3001140, highly similar
to Spondin-1 precursor.
ACCESSION AK001584
VERSION AK001584.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2802)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2802
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3001140"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000004058"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 230..1261
/codon_start=1
/note="highly similar to Spondin-1 precursor"
/protein_id="BAG50943.1"
/translation="MRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWI
TWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCLMTEWGE
WDECSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWS
DCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNK
SCGKGHVIRTRMIQMEPQFGGAPCPETVQRKKCRIRKCLRNPSIQKLRWRDARESRRS
EQLKEESEGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKE
IRACNVHPC"
BASE COUNT 767 a 661 c 686 g 688 t
ORIGIN
1 tttctatgac ccagagggtg ggtccatcac tcaagtagcc agagttgtca tcgagagaat
61 cgcacggaag ggtgaacaat gcaatattgt acctgacaat gtcgatgata ttgtagctga
121 cctggctcca gaagagaaag atgaagatga cacccctgaa acctgcatct actccaactg
181 gtccccatgg tccgcctgca gctcctccac ctgtgacaaa ggcaagagga tgcgacagcg
241 catgctgaaa gcacagctgg acctcagcgt cccctgccct gacacccagg acttccagcc
301 ctgcatgggc cctggctgca gtgacgaaga cggctccacc tgcaccatgt ccgagtggat
361 cacctggtcg ccctgcagca tctcctgcgg catgggcatg aggtcccggg agaggtatgt
421 gaagcagttc ccggaggacg gctccgtgtg cacgctgccc actgaggaaa cggagaagtg
481 cacggtcaac gaggagtgct ctcccagcag ctgcctgatg accgagtggg gcgagtggga
541 cgagtgcagc gccacctgcg gcatgggcat gaagaagcgg caccgcatga tcaagatgaa
601 ccccgcagat ggctccatgt gcaaagccga gacatcacag gcagagaagt gcatgatgcc
661 agagtgccac accatcccat gcttgctgtc cccatggtcc gagtggagtg actgcagcgt
721 gacctgcggg aagggcatgc gaacccgaca gcggatgctc aagtctctgg cagaacttgg
781 agactgcaat gaggatctgg agcaggtgga gaagtgcatg ctccctgaat gccccattga
841 ctgtgagctc accgagtggt cccagtggtc ggaatgtaac aagtcatgtg ggaaaggcca
901 cgtgattcga acccggatga tccaaatgga gcctcagttt ggaggtgcac cctgcccaga
961 gactgtgcag cgaaaaaagt gccgcatccg aaaatgcctt cgaaatccat ccatccaaaa
1021 gctacgctgg agggatgccc gagagagccg gcggagtgag cagctgaagg aagagtctga
1081 aggggagcag ttcccaggtt gtaggatgcg cccatggacg gcctggtcag aatgcaccaa
1141 actgtgcgga ggtggaattc aggaacgtta catgactgta aagaagagat tcaaaagctc
1201 ccagtttacc agctgcaaag acaagaagga gatcagagca tgcaatgttc atccttgtta
1261 gcaagggtac gagttcccca gggctgcact ctagattcca gagtcaccaa tggctggatt
1321 atttgcttgt ttaagacaat ttaaattgtg tacgctagtt ttcatttttg cagtgtggtt
1381 cgcccagtag tcttgtggat gccagagaca tcctttctga atacttcttg atgggtacag
1441 gctgagtggg gcgccctcac ctccagccag cctcttcctg cagaggagta gtgtcagcca
1501 ccttgtacta agctgaaaca tgtccctctg gagcttccac ctggccaggg aggacggaga
1561 ctttgaccta ctccacatgg agaggcaacc atgtctggaa gtgactatgc ctgagtccca
1621 gggtgcggca ggtaggaaac attcacagat gaagacagca gattccccac attctcatct
1681 ttggcctgtt caatgaaacc attgtttgcc catctcttct tagtggaact ttaggtctct
1741 tttcaagtct cctcagtcat caatagttcc tggggaaaaa cagagctggt agacttgaag
1801 aggagcattg atgttgggtg gcttttgttc tttcactgag aaattcggaa tacatttgtc
1861 tcacccctga tattggttcc tgatgccccc ccaacaaaaa taaataaata aattatggct
1921 gctttattta aatataaggt agctagtttt tacacctgag ataaataata agcttagagt
1981 gtatttttcc cttgcttttg ggggttcaga ggagtatgta caattcttct gggaagccag
2041 ccttctgaac tttttggtac taaatcctta ttggaaccaa gacaaaggaa gcaaaattgg
2101 tctctttaga gaccaatttg cctaaatttt aaaatcttcc tacacacatc tagacgttca
2161 agtttgcaaa tcagttttta gcaagaaaac atttttgcta tacaaacatt ttgctaagtc
2221 tgcccaaagc ccccccaatg cattccttca acaaaataca atctctgtac tttaaagtta
2281 ttttagtcat gaaattttat atgcagagag aaaaagttac cgagacagaa aacaaatcta
2341 agggaaagga atattatggg attaagctga gcaagcaatt ctggtggaaa gtcaaacctg
2401 tcagtgctcc acaccagggc tgtggtcctc ccagacatgc ataggaatgg ccacaggttt
2461 acactgcctt cccagcaatt ataagcacac cagattcagg gagactgacc accaagggat
2521 agtgtaaaag gacattttct cagttgggtc catcagcagt ttttcttcct gcatttattg
2581 ttgaaaacta ttgtttcatt tcttctttta taggccttat tactgcttaa tccaaatgtg
2641 taccattggt gagacacata caatgctctg aatacactac gaatttgtat taaacacatc
2701 agaatatttc caaatacaac atagtatagt cctgaatatg tacttttaac acaagagaga
2761 ctattcaata aaaactcact gggtttttca tgtctttaag ct
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