LOCUS AK001581 3268 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ10719 fis, clone NT2RP3001109.
ACCESSION AK001581
VERSION AK001581.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3268)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..3268
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3001109"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000004055"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 17..>3268
/codon_start=1
/protein_id="BAA91770.1"
/translation="MFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSK
GSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKL
DYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFK
DLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKK
VLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSP
ISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSM
RDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDV
HSHYNSVANETFCLEIMDSLRRCLSQQADVRLTLYEGFYDVLRRNSQLANSVMQTLLS
QLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQ
GEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNISAFL
VMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDS
LLSMKFVSSLLTALFRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQ
FYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNS
KEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHV
SYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKV
LEEVDWLITKLKGQVSQETLSEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALP
SGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSF
ISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKK
SKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKK"
BASE COUNT 950 a 686 c 733 g 899 t
ORIGIN
1 aatccaactc acctccatgt tcaaggatgt ccctctgact gcagaagagg tggaatttgt
61 ggtggaaaaa gcattgagca tgttctccaa gatgaatctt caagaaatac cacctttggt
121 ctatcagctt ctggttctct cctccaaggg aagcagaaag agtgttttgg aaggaatcat
181 agccttcttc agtgcactag ataagcagca caatgaggaa cagagtggtg acgagctatt
241 ggatgttgtc actgtgccat caggtgaact tcgtcatgtg gaaggcacca ttattctaca
301 cattgtgttt gccatcaaat tggactatga actaggcaga gaactcgtga aacacttaaa
361 ggtaggacag caaggagatt ccaataataa cttaagtccc ttcagcattg ctcttcttct
421 gtctgtaaca agaatacaaa gatttcagga ccaggtgctt gatcttttaa agacttcggt
481 tgtaaagagc tttaaggatc ttcaactcct ccaaggctca aaatttcttc agaatctagt
541 tcctcataga tcttatgttt caaccatgat cttggaagta gtgaagaata gcgttcatag
601 ctgggaccat gttactcagg gcctcgtaga acttggtttc attttgatgg attcatatgg
661 gccaaagaag gttcttgatg gaaaaactat tgaaaccagc ccaagtcttt ctagaatgcc
721 aaaccagcat gcatgtaagc tcggagctaa tatcctgttg gaaactttta agatccatga
781 gatgatcaga caagaaattt tggagcaggt cctcaacagg gttgttacca gagcatcttc
841 tcccatcagt catttcttag acctgctttc aaatatcgtc atgtatgcac ccttagttct
901 tcaaagttgt tcttctaaag tcacagaagc ttttgactat ttgtcctttc tgccccttca
961 gactgtacaa aggctgctta aggcagtgca gccccttctc aaagtcagca tgtcaatgag
1021 agactgcttg atacttgtcc ttcggaaagc tatgtttgcc aaccagcttg atgcccgaaa
1081 atctgcagtt gctgggtttt tgctgctcct gaagaacttt aaagttttag gcagcctgtc
1141 atcctctcag tgcagtcagt ctctcagtgt cagtcaggtt catgtggatg ttcacagcca
1201 ttacaattct gtcgccaatg aaactttttg ccttgagatc atggatagtt tgaggagatg
1261 cttaagccag caagctgatg ttcgactcac gctttatgag gggttttatg atgttcttcg
1321 aaggaactct cagctggcta attcagtcat gcaaactctg ctctcacagt taaaacagtt
1381 ctatgagcca aaacctgatc tgctgcctcc tctgaaatta gaagcttgta ttctgaccca
1441 aggagataag atctctctac aagaaccact ggattatctg ctgtgttgta ttcagcattg
1501 tttggcctgg tataagaata cagtcatacc cttacagcag ggagaggagg aagaggagga
1561 ggaagaggca ttctacgaag acctagatga tatattggag tccattacta atagaatgat
1621 taagagtgag ctggaagact ttgaactgga taaatcagca gatttttctc agagcaccag
1681 tattggcata aaaaataata tctctgcttt tcttgtgatg ggagtttgtg aggttttaat
1741 agaatacaat ttctccataa gtagtttcag taagaatagg tttgaggaca ttctgagctt
1801 atttatgtgt tacaaaaaac tctctgacat tcttaatgaa aaagcgggta aagccaaaac
1861 taaaatggcc aacaagacaa gtgatagtct tttgtccatg aaatttgtgt ccagtcttct
1921 cactgctctt ttcagagtct tgctatggag atacacttca attcctactt cagtggaaga
1981 gtcgggaaag aaagagaaag gaaagagcat ctcactgctg tgcttggagg gtttacagaa
2041 aatattcagt gctgtgcaac agttctatca gcccaagatt cagcagtttc tcagagctct
2101 ggatgtcaca gataaggaag gagaagagag agaagatgca gatgtcagtg tcactcagag
2161 aacagcattc cagatccggc aatttcagag gtccttgttg aatttactta gcagtcaaga
2221 ggaagatttt aatagcaaag aagccctcct gctagtcacg gttcttacca gtttgtccaa
2281 gttactggag ccctcctctc ctcagtttgt gcagatgtta tcctggacat caaagatttg
2341 caaggaaaac agccgggagg atgccttgtt ttgcaagagc ttgatgaact tgctcttcag
2401 cctgcatgtt tcgtataaga gtcctgtcat tctgctgcgt gacttgtccc aggatatcca
2461 cgggcatctg ggagatatag accaggatgt agaggtggag aaaacaaacc actttgcaat
2521 agtgaatttg agaacggctg cccccactgt ctgtttactt gttctgagtc aggccgagaa
2581 ggttctagaa gaagtggact ggctaatcac caagcttaag ggacaagtga gccaagaaac
2641 cttatcagag gcctcttctc aggcaaccct accaaatcag cctgttgaga aagctatcat
2701 catgcaactg ggaactctgc ttacattttt ccacgagctg gtgcagacag ctctgccatc
2761 aggcagctgt gtggacacct tgttaaagga cttgtgcaaa atgtacacca cacttacagc
2821 ccttgtcaga tattatctcc aggtgtgtca gagctccgga ggaattccaa aaaatatgga
2881 aaagctggtg aagctgtctg gttctcatct gacccccctg tgttattctt tcatttctta
2941 cgtacagaat aagagtaaga gcctgaacta tacgggagag aaaaaggaga aacctgctgc
3001 cgttgccaca gccatggcca gagttcttcg ggaaaccaag ccaatcccta acctcatctt
3061 tgccatagaa cagtatgaaa aatttctcat ccacctttct aagaagtcca aggtgaacct
3121 gatgcagcac atgaagctca gcacctcacg agacttcaag atcaaaggaa acatcctaga
3181 catggttctt cgagaggatg gcgaagatga aaatgaagag ggcactgcat cagagcatgg
3241 gggacagaac aaagaaccag ccaagaag
//