LOCUS AK001183 1670 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ10321 fis, clone NT2RM2000504, highly similar
to Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), mRNA.
ACCESSION AK001183
VERSION AK001183.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1670)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..1670
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM2000504"
/clone_lib="NT2RM2"
/db_xref="H-InvDB:HIT000003657"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 38..1369
/codon_start=1
/note="highly similar to Homo sapiens pitrilysin
metallopeptidase 1 (PITRM1), mRNA"
/protein_id="BAG50863.1"
/translation="MVYFRAFSSLNTLPEELRPYVPPFCSILTKLGCGLLDYREQAQQ
IELKTGGMSASPHVLPDDSHMDTYEQGVLFSSLCLDRNLPDMMQLWSEIFNNPCFEEE
EHFKVLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGDLQETFSGMDQVRLMKRIAE
MTDIKPILRKLPRIKKHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRP
VRPHTVEKPVPSSSGGDAHVPHGSQVIRKLVMEPTFKPWQMKTHFLMPFPVNYVGECI
RTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLSHNGIFTLYSYRDPNT
IETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLYGLSDEMK
QAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIR"
BASE COUNT 443 a 454 c 415 g 358 t
ORIGIN
1 tatccctgtt cagtactgcg cccagcccac caatggcatg gtgtatttcc gggccttctc
61 cagcctgaac acactccccg aggagctgag gccctatgtg ccccccttct gcagcatcct
121 caccaagctg ggctgcggcc ttcttgacta ccgggagcag gctcagcaga tagaattgaa
181 gaccggaggg atgagtgctt ctccccacgt gctccccgac gactcacaca tggacaccta
241 cgagcagggt gtgcttttct cctctctctg cctggatcga aacctgccag acatgatgca
301 gctatggagt gaaatattta acaacccgtg ctttgaagaa gaggagcact tcaaggtgct
361 ggtgaagatg accgcccagg agctcgccaa tggaattcct gactctgggc acctgtacgc
421 atccatcagg gcaggccgga ccctcacgcc cgcaggggac ctgcaggaga ccttcagcgg
481 gatggatcag gtgcggctga tgaagaggat tgcagaaatg acagatatca aacccatcct
541 gaggaagctc ccgcgtatca agaaacactt gttaaatggt gataatatga ggtgttcagt
601 gaatgcgact cctcagcaga tgcctcagac agaaaaagcg gtcgaagact tccttagaag
661 catcggtcgg agtaaaaagg aacggaggcc tgtgcgccca cacacggtcg agaaacctgt
721 gcccagcagc tctggtggag atgcccacgt tccccatggc tcccaggtca ttaggaagct
781 ggtcatggaa cccaccttca agccctggca gatgaagact cacttcctga tgcccttccc
841 ggtgaattac gtgggtgaat gcatccgaac tgtcccctac acggacccag atcatgccag
901 tcttaaaatc cttgcacgtt tgatgactgc caaattcttg catacagaaa ttcgagaaaa
961 aggcggtgct tatggtggag gcgcaaaact cagccacaat gggattttca ccctttactc
1021 ttacagggac ccaaatacaa tagagacgct ccagtctttt gggaaggctg tcgactgggc
1081 taagtctgga aaattcacac agcaagacat cgacgaagcc aaactttctg tcttctcaac
1141 cgtagatgct cctgtcgctc cttcagacaa aggaatggac cacttcttgt acggcctctc
1201 ggatgagatg aagcaggccc acagagagca gctctttgct gtcagccacg acaagctcct
1261 ggccgtgagc gataggtacc tcggcactgg gaagagcaca cacggcctgg ccatcctcgg
1321 acccgagaac ccgaaaattg ccaaggaccc atcctggatc atccgatgag cagccgtggc
1381 gctcgactgc acaggagccc gagacaatac acctccaagc tgaatatgaa aagtcagaaa
1441 tgctactgct ttttccaaga atattatgtc attgagtgtc gccaaagccc ttgactggcg
1501 agtcaaaaac tcagatctat cttaagagtg accaggaaga ggttcattga aataatcatg
1561 catgaagcgc caaagatgca ccatgtagaa ttttcacttt gtactggcag gctcgtttta
1621 cctgattcta gaatatttaa gaatctaaaa ataaagggca actctgactt
//