LOCUS AK000280 4020 bp mRNA linear HUM 12-SEP-2006
DEFINITION Homo sapiens cDNA FLJ20273 fis, clone HEP01750.
ACCESSION AK000280
VERSION AK000280.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4020)
AUTHORS Sugano,S., Suzuki,Y., Ota,T., Obayashi,M., Nishi,T., Isogai,T.,
Shibahara,T., Tanaka,T. and Nakamura,Y.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Sumio Sugano
Institute of Medical Science, University of Tokyo, Deptment of
Virology; Shirokane-dai, 4-6-1, Minato-ku, Tokyo 108-8639, Japan
REFERENCE 2
AUTHORS Kawakami,T., Noguchi,S., Itoh,T., Shigeta,K., Senba,T.,
Matsumura,K., Nakajima,Y., Mizuno,T., Morinaga,M., Ota,T.,
Suzuki,Y., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Nakamura,Y., Isogai,T. and Sugano,S.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing: Departent of
Virology and Human Genome Center, Institute of Medical Science,
University of Tokyo (partly supported by Science and Technology
Agency).
FEATURES Location/Qualifiers
source 1..4020
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP01750"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000002755"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector pME18SFL3"
/organism="Homo sapiens"
CDS 240..1814
/codon_start=1
/protein_id="BAA91049.1"
/translation="MTAEDSTAAMSSDSAAGSSAKVPEGVAGAPNEAALLALMERTGY
SMVQENGQRKYGGPPPGWEGPHPQRGCEVFVGKIPRDVYEDELVPVFEAVGRTYELRL
MMDFDGKNRGYAFVMYCHKHEAKRAVRELNNYEIRPGRLLGVCCSVDNCRLFIGGIPK
MKKREEILEEIAKVTEGVLDVIVYASAADKMKNRGFAFVEYESHRAAAMARRKLMPGR
IQLWGHQIAVDWAEPEIDVDEDVMETVKILYVRNLMIETTEDTIKKSFGQFNPGCVER
VKKIRDYAFVHFTSREDAVHAMNNLNGTELEGSCLEVTLAKPVDKEQYSRYQKAARGG
GAAEAAQQPSYVYSCDPYTLAYYGYPYNALIGPNRDYFVKVAIPAIGAQYSMFPAAPA
PKMIEDGKIHTVEHMISPIAVQPDPASAAAAAAAAAAAAAAVIPTVSTPPPFQGRPIT
PVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNAAAAAAVYGGYAGYIPQAF
PAAAIQVPIPDVYQTY"
BASE COUNT 1050 a 950 c 951 g 1069 t
ORIGIN
1 gtcaattgcc ctttagtccc aggactaacc ggaagcttct gcaacaggag gacattgaaa
61 ataagatgga acccatccac ataaggattt gcctcaaagg gcactgcaaa aattgaacag
121 aggaatccca aggaagctgc ctgaatttgc ctgtatactc tcgttctgcg acttataaag
181 gaccagacaa atcaaattag tggttttggt ttccgccagc tgtggatgcc tttgacatta
241 tgaccgcaga ggattccacc gcagccatga gcagtgactc ggccgccggg tcctcggcca
301 aggtgcccga gggcgtggcg ggcgcgccca acgaggcagc actgctggcg ctgatggagc
361 gcacgggcta cagcatggtg caagagaacg ggcagcgcaa gtacggcggc ccaccgcccg
421 gctgggaggg cccgcacccg cagcgtggct gcgaggtctt cgtgggcaag atcccgcgcg
481 acgtgtacga ggacgagctg gtgcccgtgt tcgaggccgt gggccgcacc tacgagctgc
541 gcctcatgat ggactttgac ggcaagaacc gcggctacgc cttcgtcatg tactgccaca
601 agcacgaggc caagcgcgca gtgcgtgagc tcaacaacta cgagatccgc ccgggccgcc
661 tgctcggcgt gtgctgcagc gtggacaact gccgcctctt catcggcggg atccccaaga
721 tgaagaagcg cgaggaaatc ctggaggaga ttgccaaggt caccgagggc gtgctggacg
781 tgatcgtcta cgccagcgcg gccgacaaga tgaagaaccg cggcttcgcc ttcgtggagt
841 acgagagcca ccgcgcggct gccatggctc gccgcaagct catgcctggc cgcatccagc
901 tgtggggcca ccagatcgcc gtggactggg ccgagcctga gatcgacgtg gacgaggacg
961 tgatggagac cgtgaagatc ctctacgtgc gcaacctcat gatcgagacc accgaggaca
1021 ccatcaagaa gagcttcggc cagttcaacc ccggctgcgt ggagcgcgtc aagaagatcc
1081 gcgactacgc cttcgtgcac ttcaccagcc gcgaggatgc cgtgcatgcc atgaacaacc
1141 tcaacggcac tgagctggag ggctcgtgcc tggaggtcac gctggccaag cccgtggaca
1201 aggagcagta ctcgcgctac cagaaggcag ccaggggcgg cggcgcggct gaggcagcgc
1261 agcagcccag ctacgtgtac tcctgcgacc cctacacact ggcctactac ggctacccct
1321 acaacgcgct cattgggccc aacagggact actttgtgaa agtagccatc cctgccattg
1381 gggctcagta ttccatgttt ccagcagctc cagcccctaa aatgattgaa gatggcaaaa
1441 tccacacagt ggagcacatg atcagcccca ttgctgtgca gccagaccca gccagtgctg
1501 ctgccgccgc agccgcggcc gcagccgccg cagccgctgt cattcccact gtgtcgacgc
1561 caccaccttt ccagggccgc ccaataactc cagtatacac ggtggctcca aacgttcaga
1621 gaattcctac tgccgggatc tacggggcca gttacgtgcc atttgctgct ccagctacag
1681 ccacgatcgc cacactacag aagaacgcgg cagccgcggc cgccgtgtat ggaggatacg
1741 caggctacat acctcaggcc ttccctgctg ctgccattca ggtccccatc cccgacgtct
1801 accagacata ctgaggctgg tgaccagcac gaagacagac cacacaaaca ccactgaagg
1861 aacgcttgac tatttatgaa gaaggaacat gttggattca cacatgcaac ctgaaagtga
1921 agaatgttag cagatttatt tctgaattat tttatataca tgaagttttc actagttttt
1981 taagactatt ttcaacttag catgcctacg ttcatacatt tccaaaagac ttgcaatggt
2041 tcgtgccttc attccatctt ttaaaaattt gtatgctgta ctacatttgt atagaggttt
2101 ttgttgttgt ttttttaagg atatattttc agtatgaagg ttattttctt aacttctgca
2161 ctccagagat ttctattttg tagtaccttc aataatatat caactatata ttaaaaaagc
2221 acacttgagg agctagggaa ctattttgaa aaatatatac aatatttaaa gatacaaaca
2281 gtagtgctta aaaatactac ataaagcatt attttaaagg ttatactgga aagtgcaatt
2341 ttaaaatgag taaaacctct gtatttctgc tggcattaag ggttgatggt gttaccatgt
2401 atcatcatgg cggtactatt ttttaaaaga aattaaacac tggatctctc cttaagccaa
2461 cattgaaaag acttgccgca cttctgagtc caaacactgg aaagctctcc ttgccaccgt
2521 tagccggggc tcattctcca tgtgccttag ccttaaacat gcccccactc ccacatctct
2581 caccctgtcc cctcctcccc agattcccaa tcccaccgca atgtttggca agcctaggac
2641 tgataagtag ctctgataga ggagctggtg gcttttatac ttcttcctgg gtttttgttg
2701 gggtttgttg tttcgttgtt ttttgttttt tttttgtttg gttggggaag tattgtcttc
2761 tacgtgtgct attttcagta gcagagtaag cacaaggttt taatcgagtt gcataagaca
2821 cctttgcata gctatttaat tgcccaatgt aaaactttaa tgccatttct aatgctttta
2881 ttcatttttg aagtatgagt ttgtagggac aaagaatgta tgttatcgta gacaagaccc
2941 ccagagactc ttttcagcag aaagttatgc ttctagttgc cttaccatgt ttcttgcaaa
3001 actgtccatg gtcctcaagg gtgttggaaa cattatgttt attaaatggg cctctcttcc
3061 tttgctgtgc acttgatggg tgaactggat tggggtgtgc acatccagga ggaggaggag
3121 agacctgtag aagtttaaag atagtttgta aatatcttct aatgcttgtt tttagtcctt
3181 ttatgttgga gaagttcatg gtatgtagtt taatgcaaaa tgaaaccatt ttatttcaat
3241 gttattaaaa aggtttgttt tattaggaag ttaatgtatt gttgcagtgt tttgtgcctg
3301 tttaaaggct tttgtttagc agagtgaatg taaaatacag taaaatgtta agattgtcat
3361 ctacttttta aaaaaaaata tcaacttgga attgtttttt aaaggctcaa tcaaggaagt
3421 gaggtgtgca ataaggtagc aagtaaaacg cagttgcgtt tttatgtcat gttagagatc
3481 catacaattt tccactcacg ggatttttgt tgatggctga attcttgtgg attcataaga
3541 ggatcatgcc cttagcaagt acttttgttt tgttttaaat taagagattc ccaaatgcct
3601 ttttccccct catcttgaaa tgagatgagt ttttatgtgt aagcaatatt tatttaacta
3661 ttctataaaa ttattgagtg cctactgagg cctttaagca ccgctaacat tcctttccat
3721 cattcttttg aatgacataa aataattgtg caatgttcct gatgatgtac cccacagctg
3781 cattcaaact caaatctgtg ggaatgagtg actcgaccaa atgtaattcg gatcagatcc
3841 tcatcccctg actgtgtgaa aaaagtactc tccttctagt gaaggattgt cacagagttt
3901 cactggatga aactatgacc cagtattctt actgtatttt acatgtgcct gtaaattatt
3961 ttgccgaaat aagaagaaga agaggaagaa agaacagtag aaaaaaaaaa taaaaaaaaa
//