LOCUS       AI763350                 486 bp    mRNA    linear   EST 05-JAN-2011
DEFINITION  wi55e04.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE:2394174 3',
            mRNA sequence.
ACCESSION   AI763350
VERSION     AI763350.1
DBLINK      BioSample: SAMN00156117
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 486)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Ilan Kirsch, M.D., Michael R. Emmert-Buck,
            M.D., Ph.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 1614   Std Error: 0.00
            Seq primer: -40UP from Gibco
            High quality sequence stop: 401.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2394174"
                     /tissue_type="colon tumor, RER+"
                     /clone_lib="SAMN00156117 NCI_CGAP_Co16"
                     /lab_host="DH10B"
                     /note="Organ: colon; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Plasmid DNA from the normalized library
                     NCI_CGAP_Co10 was prepared, and ss circles were made in
                     vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from a pool of 5,000 clones made
                     from the same library (cloneIDs 1057416-1061255, and
                     1144584-1145351). Subtraction by Bento Soares and M.
                     Fatima Bonaldo."
BASE COUNT           65 a          194 c          174 g           53 t
ORIGIN      
        1 gtcgggccgc gcgggagccc ccgaggcgca tggtcccgcg gcgcccggca cgacagcccc
       61 ggaggggggc gaccgctgcc gcggctacta cgacgtgatg ggccagtggg acccgccctt
      121 caactgcagc tccggagcct acagcttctg ctgcggcacg tgcggctacc gcttctgctg
      181 ccacgacggg ccgcggcgcc tcgaccagag ccgctgttcc aactacgaca cgccggcctg
      241 ggtccagaca ggccggccgc ccgcccgcgc ccgcgacacc gcagcgcccc gggaccccgg
      301 ccgcgagcgc agccatacgg ccgtctacgc tgtgtgcggc gtcgcagcgc tgctggtgct
      361 ggccggcatc ggggcgcgcc tgggactgga gagggcgcac agcccgcgcg cgcggcgcac
      421 agtgaccagg gcgctgacag agcttctgaa gcagccgggc ccccaggagc cactgcctcc
      481 caccct
//