LOCUS AI763350 486 bp mRNA linear EST 05-JAN-2011 DEFINITION wi55e04.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE:2394174 3', mRNA sequence. ACCESSION AI763350 VERSION AI763350.1 DBLINK BioSample: SAMN00156117 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 486) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Ilan Kirsch, M.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1614 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 401. FEATURES Location/Qualifiers source 1..486 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2394174" /tissue_type="colon tumor, RER+" /clone_lib="SAMN00156117 NCI_CGAP_Co16" /lab_host="DH10B" /note="Organ: colon; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Co10 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1057416-1061255, and 1144584-1145351). Subtraction by Bento Soares and M. Fatima Bonaldo." BASE COUNT 65 a 194 c 174 g 53 t ORIGIN 1 gtcgggccgc gcgggagccc ccgaggcgca tggtcccgcg gcgcccggca cgacagcccc 61 ggaggggggc gaccgctgcc gcggctacta cgacgtgatg ggccagtggg acccgccctt 121 caactgcagc tccggagcct acagcttctg ctgcggcacg tgcggctacc gcttctgctg 181 ccacgacggg ccgcggcgcc tcgaccagag ccgctgttcc aactacgaca cgccggcctg 241 ggtccagaca ggccggccgc ccgcccgcgc ccgcgacacc gcagcgcccc gggaccccgg 301 ccgcgagcgc agccatacgg ccgtctacgc tgtgtgcggc gtcgcagcgc tgctggtgct 361 ggccggcatc ggggcgcgcc tgggactgga gagggcgcac agcccgcgcg cgcggcgcac 421 agtgaccagg gcgctgacag agcttctgaa gcagccgggc ccccaggagc cactgcctcc 481 caccct //