LOCUS       AI743601                 544 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  wg51e12.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone
            IMAGE:2368654 3' similar to WP:Y38A8.1 CE07570, mRNA sequence.
ACCESSION   AI743601
VERSION     AI743601.1
DBLINK      BioSample: SAMN00155770
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 544)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 942   Std Error: 0.00
            Seq primer: -40UP from Gibco
            High quality sequence stop: 462.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2368654"
                     /clone_lib="SAMN00155770 Soares_NSF_F8_9W_OT_PA_P_S1"
                     /lab_host="DH10B"
                     /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; Equal amounts of plasmid DNA from five
                     normalized libraries were mixed, and ss circles were made
                     in vitro. Following HAP purification, this DNA was used as
                     tracer in a subtractive hybridization reaction. The driver
                     was PCR-amplified cDNAs from pools of 5,000 clones made
                     from the same 5 libraries. The pools consisted of the
                     following libraries and cloneIDs: Soares NbHSF pool 1:
                     309384-310919, 323208-325895 Soares Nb2HP pool 1:
                     145032-147335, 147720-148103, 148872-149255, 15002 -
                     150407, 151176-152327 Soares Nb2HF8-9W pool 1:
                     758280-760583, 772104-774407 Soares NbHPA pool 1:
                     304776-306311, 320136-322823, 326280-326663 Soares NbHOT
                     pool 1: 723720-726407, 739080-740999 Subtraction by Bento
                     Soares and M. Fatima Bonaldo."
BASE COUNT           99 a          165 c          180 g          100 t
ORIGIN      
        1 tttagagctg tcaaagacct ttattcagtc ctggagtgga gctggcctga cagtgtgaag
       61 ccaggggctg cttagggcgg gccagaccct cagcccctga gcaggtcagg ccggccaggc
      121 ggggctggta ggtaccaggt gccagcagct gcagggcaaa ctcagtgatg acagctgtga
      181 ggccccagac ccggtgtggt ccatgcagga agacgggtag tgtgtagtgg aagtggccac
      241 cccggcagaa gtgggtatag ccctgattct gcgtctgcag caggtgggcc agcggcagtg
      301 caaacacttc atctacctcc tccgagttgg gcctgaggct ctggggatcc agtgggccta
      361 caccagcaag cactggcacc acggtggcct tttgcggatc atacacaggc cgcagcaggc
      421 cccacacgtg ctcctcgggc actgccaggc ccagctcctc ccgggtttcc cgcagggccg
      481 tgtgcaccac atcttggtca gccgggtcgc acttgccgcc tgggaaactg acgtcgccct
      541 tgtg
//