LOCUS AI743601 544 bp mRNA linear EST 08-JAN-2011 DEFINITION wg51e12.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:2368654 3' similar to WP:Y38A8.1 CE07570, mRNA sequence. ACCESSION AI743601 VERSION AI743601.1 DBLINK BioSample: SAMN00155770 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 544) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 942 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 462. FEATURES Location/Qualifiers source 1..544 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2368654" /clone_lib="SAMN00155770 Soares_NSF_F8_9W_OT_PA_P_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from five normalized libraries were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 5 libraries. The pools consisted of the following libraries and cloneIDs: Soares NbHSF pool 1: 309384-310919, 323208-325895 Soares Nb2HP pool 1: 145032-147335, 147720-148103, 148872-149255, 15002 - 150407, 151176-152327 Soares Nb2HF8-9W pool 1: 758280-760583, 772104-774407 Soares NbHPA pool 1: 304776-306311, 320136-322823, 326280-326663 Soares NbHOT pool 1: 723720-726407, 739080-740999 Subtraction by Bento Soares and M. Fatima Bonaldo." BASE COUNT 99 a 165 c 180 g 100 t ORIGIN 1 tttagagctg tcaaagacct ttattcagtc ctggagtgga gctggcctga cagtgtgaag 61 ccaggggctg cttagggcgg gccagaccct cagcccctga gcaggtcagg ccggccaggc 121 ggggctggta ggtaccaggt gccagcagct gcagggcaaa ctcagtgatg acagctgtga 181 ggccccagac ccggtgtggt ccatgcagga agacgggtag tgtgtagtgg aagtggccac 241 cccggcagaa gtgggtatag ccctgattct gcgtctgcag caggtgggcc agcggcagtg 301 caaacacttc atctacctcc tccgagttgg gcctgaggct ctggggatcc agtgggccta 361 caccagcaag cactggcacc acggtggcct tttgcggatc atacacaggc cgcagcaggc 421 cccacacgtg ctcctcgggc actgccaggc ccagctcctc ccgggtttcc cgcagggccg 481 tgtgcaccac atcttggtca gccgggtcgc acttgccgcc tgggaaactg acgtcgccct 541 tgtg //