LOCUS       AI494442                 253 bp    mRNA    linear   EST 09-JAN-2011
DEFINITION  qy99g03.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE:2020180 3'
            similar to contains Alu repetitive element;contains TAR1.t1 TAR1
            repetitive element, mRNA sequence.
ACCESSION   AI494442
VERSION     AI494442.1
DBLINK      BioSample: SAMN00155914
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 253)
  CONSRTM   NCI/NINDS-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute / National Institute of Neurological
            Disorders and Stroke, Brain Tumor Genome Anatomy Project
            (CGAP/BTGAP), Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: David N. Louis, M.D., Myrna R. Rosenfeld M.D.,
            Ph.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 414   Std Error: 0.00
            Seq primer: -40UP from Gibco.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2020180"
                     /tissue_type="anaplastic oligodendroglioma"
                     /clone_lib="SAMN00155914 NCI_CGAP_Brn25"
                     /lab_host="DH10B"
                     /note="Organ: brain; Vector: pT7T3D-PacI; Site_1: Not I;
                     Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATAGGTTTTTTTTTTTTTTTTTTTTTTTT
                     T 3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library is
                     normalized, and was constructed by Bento Soares and
                     M.Fatima Bonaldo."
BASE COUNT           34 a          100 c          101 g           18 t
ORIGIN      
        1 gcgcccgccg tgtgagcagc agcagcggct ggtctgtcaa ccggagcccg agcccgagca
       61 gcctgcggcc agcagcgtcc tcgcaagccg agcgcccagg cgcgccagga gcccgcagca
      121 gcggcagcag cgcgccgggc cgcccgggaa gcctccgtcc ccgcggcggc ggcggcggcg
      181 gcggcaacat ggcctcggct ggtaacgccg ccgagcccca ggaccgcggc ggcggcggca
      241 gcggctgtat cgg
//