LOCUS AI494442 253 bp mRNA linear EST 09-JAN-2011 DEFINITION qy99g03.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE:2020180 3' similar to contains Alu repetitive element;contains TAR1.t1 TAR1 repetitive element, mRNA sequence. ACCESSION AI494442 VERSION AI494442.1 DBLINK BioSample: SAMN00155914 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 253) CONSRTM NCI/NINDS-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute / National Institute of Neurological Disorders and Stroke, Brain Tumor Genome Anatomy Project (CGAP/BTGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: David N. Louis, M.D., Myrna R. Rosenfeld M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 414 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..253 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2020180" /tissue_type="anaplastic oligodendroglioma" /clone_lib="SAMN00155914 NCI_CGAP_Brn25" /lab_host="DH10B" /note="Organ: brain; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATAGGTTTTTTTTTTTTTTTTTTTTTTTT T 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 34 a 100 c 101 g 18 t ORIGIN 1 gcgcccgccg tgtgagcagc agcagcggct ggtctgtcaa ccggagcccg agcccgagca 61 gcctgcggcc agcagcgtcc tcgcaagccg agcgcccagg cgcgccagga gcccgcagca 121 gcggcagcag cgcgccgggc cgcccgggaa gcctccgtcc ccgcggcggc ggcggcggcg 181 gcggcaacat ggcctcggct ggtaacgccg ccgagcccca ggaccgcggc ggcggcggca 241 gcggctgtat cgg //