LOCUS       AI366170                 470 bp    mRNA    linear   EST 07-JAN-1999
DEFINITION  ap19c05.x1 Schiller oligodendroglioma Homo sapiens cDNA clone
            IMAGE:1955816 3' similar to TR:Q62711 Q62711 PHOSPHOLIPASE C
            DELTA-4. [1], mRNA sequence.
ACCESSION   AI366170
VERSION     AI366170.1
DBLINK      BioSample: SAMN00155987
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
            Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
            Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-NCI human EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Possible reversed clone: similarity on wrong strand
            Seq primer: -40UP from Gibco
            High quality sequence stop: 419.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1955816"
                     /sex="male"
                     /tissue_type="oligodendroglioma"
                     /clone_lib="SAMN00155987 Schiller oligodendroglioma"
                     /dev_stage="44 years"
                     /lab_host="SOLR"
                     /note="Organ: brain; Vector: pBluescript SK- (Stratagene);
                     Site_1: EcoRI; Site_2: XhoI; Double-stranded cDNA was
                     prepared from human oligodendroglioma using primer
                     5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI
                     adaptor was used on the 5' end of the cDNA as follows:
                     5'-AATTCGGCACGAG-3'. The library was size-selected and
                     went through one round of amplification. Average insert
                     size is 1.7 kb, with a range from 0.4-12 kb. Tumor
                     identification by consensus pathology; contains chromosome
                     1p and 19q deletion as determined by CGH. This library was
                     constructed by Dr. Martin Schiller (Johns Hopkins
                     University)."
BASE COUNT          117 a          130 c          112 g          111 t
ORIGIN      
        1 tccccgtcta aagccagcac accaacgctt cccgtgaaat gcccacctca ggactcatcc
       61 ccctccaggc cttcctggat gcagatatac acaaagatgg aagctgggcg gaggctgatg
      121 ccatctttgg acagcaggtg aatgtggcgg taaccttgtt gcatgcaggt ccaaggcagg
      181 gtgtactgac caataaagtc atttcgggat ttccagtcat aatccattac cacaaaacgc
      241 agcatggcaa gttcaggcac cagcacccgg aaacatagtg tctgccccca gtatggatta
      301 aaaccattgt tctccacata gttggtctcc tgccgtgctg tgtctagacg aacgccaaag
      361 atctgcactt tcaccagtgg atccacaatg gacccctctt tggtcttgtc cactttgggg
      421 agttgctgac cgctgatcac ctggattaag agagtctggg cttttaaaag
//