LOCUS AI366170 470 bp mRNA linear EST 07-JAN-1999 DEFINITION ap19c05.x1 Schiller oligodendroglioma Homo sapiens cDNA clone IMAGE:1955816 3' similar to TR:Q62711 Q62711 PHOSPHOLIPASE C DELTA-4. [1], mRNA sequence. ACCESSION AI366170 VERSION AI366170.1 DBLINK BioSample: SAMN00155987 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 470) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Possible reversed clone: similarity on wrong strand Seq primer: -40UP from Gibco High quality sequence stop: 419. FEATURES Location/Qualifiers source 1..470 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1955816" /sex="male" /tissue_type="oligodendroglioma" /clone_lib="SAMN00155987 Schiller oligodendroglioma" /dev_stage="44 years" /lab_host="SOLR" /note="Organ: brain; Vector: pBluescript SK- (Stratagene); Site_1: EcoRI; Site_2: XhoI; Double-stranded cDNA was prepared from human oligodendroglioma using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; contains chromosome 1p and 19q deletion as determined by CGH. This library was constructed by Dr. Martin Schiller (Johns Hopkins University)." BASE COUNT 117 a 130 c 112 g 111 t ORIGIN 1 tccccgtcta aagccagcac accaacgctt cccgtgaaat gcccacctca ggactcatcc 61 ccctccaggc cttcctggat gcagatatac acaaagatgg aagctgggcg gaggctgatg 121 ccatctttgg acagcaggtg aatgtggcgg taaccttgtt gcatgcaggt ccaaggcagg 181 gtgtactgac caataaagtc atttcgggat ttccagtcat aatccattac cacaaaacgc 241 agcatggcaa gttcaggcac cagcacccgg aaacatagtg tctgccccca gtatggatta 301 aaaccattgt tctccacata gttggtctcc tgccgtgctg tgtctagacg aacgccaaag 361 atctgcactt tcaccagtgg atccacaatg gacccctctt tggtcttgtc cactttgggg 421 agttgctgac cgctgatcac ctggattaag agagtctggg cttttaaaag //