LOCUS       AI272212                 456 bp    mRNA    linear   EST 18-NOV-1998
DEFINITION  ap22b02.x1 Schiller oligodendroglioma Homo sapiens cDNA clone
            IMAGE:1956075 3' similar to TR:Q61366 Q61366 C10A, mRNA sequence.
ACCESSION   AI272212
VERSION     AI272212.1
DBLINK      BioSample: SAMN00155987
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
            Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
            Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-NCI human EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Possible reversed clone: similarity on wrong strand
            Possible reversed clone: polyT not found
            Seq primer: -40UP from Gibco
            High quality sequence stop: 449.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1956075"
                     /sex="male"
                     /tissue_type="oligodendroglioma"
                     /clone_lib="SAMN00155987 Schiller oligodendroglioma"
                     /dev_stage="44 years"
                     /lab_host="SOLR"
                     /note="Organ: brain; Vector: pBluescript SK- (Stratagene);
                     Site_1: EcoRI; Site_2: XhoI; Double-stranded cDNA was
                     prepared from human oligodendroglioma using primer
                     5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI
                     adaptor was used on the 5' end of the cDNA as follows:
                     5'-AATTCGGCACGAG-3'. The library was size-selected and
                     went through one round of amplification. Average insert
                     size is 1.7 kb, with a range from 0.4-12 kb. Tumor
                     identification by consensus pathology; contains chromosome
                     1p and 19q deletion as determined by CGH. This library was
                     constructed by Dr. Martin Schiller (Johns Hopkins
                     University)."
BASE COUNT           78 a          169 c          144 g           65 t
ORIGIN      
        1 cgagcggcac gagcagcccc cggtctcgtc tgggggtctg cggggctagc tttggctgcg
       61 gtgaccccac tccctcccca gagcatcccg agggccccgt cttcggacga ggagtgcttc
      121 tttgacctgt tgaccaagtt ccagagcagc cgcatggacg accagcgttg tcccctggac
      181 gatggccagg ccggggctgc cgaggccacg gccgccccca ccctggagga caggatcgcc
      241 cagccctcga tgacggcctc gccccagacc gaggaattct tcgacctcat cgccagctcc
      301 cagagccgcc ggctggacga ccagcgggcc agcgtgggca gcctgccggg gctgcgaatc
      361 acccacagca atgcagggca cctccgaggc cacggcgagc cccaggagcc gggggacgac
      421 ttcttcaaca tgctcatcaa gtaccagtcc tccagg
//