LOCUS AI272212 456 bp mRNA linear EST 18-NOV-1998 DEFINITION ap22b02.x1 Schiller oligodendroglioma Homo sapiens cDNA clone IMAGE:1956075 3' similar to TR:Q61366 Q61366 C10A, mRNA sequence. ACCESSION AI272212 VERSION AI272212.1 DBLINK BioSample: SAMN00155987 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 456) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Possible reversed clone: similarity on wrong strand Possible reversed clone: polyT not found Seq primer: -40UP from Gibco High quality sequence stop: 449. FEATURES Location/Qualifiers source 1..456 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1956075" /sex="male" /tissue_type="oligodendroglioma" /clone_lib="SAMN00155987 Schiller oligodendroglioma" /dev_stage="44 years" /lab_host="SOLR" /note="Organ: brain; Vector: pBluescript SK- (Stratagene); Site_1: EcoRI; Site_2: XhoI; Double-stranded cDNA was prepared from human oligodendroglioma using primer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; contains chromosome 1p and 19q deletion as determined by CGH. This library was constructed by Dr. Martin Schiller (Johns Hopkins University)." BASE COUNT 78 a 169 c 144 g 65 t ORIGIN 1 cgagcggcac gagcagcccc cggtctcgtc tgggggtctg cggggctagc tttggctgcg 61 gtgaccccac tccctcccca gagcatcccg agggccccgt cttcggacga ggagtgcttc 121 tttgacctgt tgaccaagtt ccagagcagc cgcatggacg accagcgttg tcccctggac 181 gatggccagg ccggggctgc cgaggccacg gccgccccca ccctggagga caggatcgcc 241 cagccctcga tgacggcctc gccccagacc gaggaattct tcgacctcat cgccagctcc 301 cagagccgcc ggctggacga ccagcgggcc agcgtgggca gcctgccggg gctgcgaatc 361 acccacagca atgcagggca cctccgaggc cacggcgagc cccaggagcc gggggacgac 421 ttcttcaaca tgctcatcaa gtaccagtcc tccagg //