LOCUS AF250924 2182 bp mRNA linear HUM 17-MAY-2002
DEFINITION Homo sapiens PNGase mRNA, complete cds.
ACCESSION AF250924
VERSION AF250924.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2182)
AUTHORS Suzuki,T., Park,H., Hollingsworth,N.M., Sternglanz,R. and
Lennarz,W.J.
TITLE PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase
JOURNAL J. Cell Biol. 149 (5), 1039-1052 (2000)
PUBMED 10831608
REFERENCE 2 (bases 1 to 2182)
AUTHORS Suzuki,T. and Lennarz,W.J.
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2000) Biochem. &. Cell Biol., State University of
New York at Stony Brook, 450 Life Sci. Bldg., Dept. Biochem. Cell
Biol., State University of New York at Stony Brook, Stony Brook, NY
11794-5215, USA
REFERENCE 3 (bases 1 to 2182)
AUTHORS Suzuki,T. and Lennarz,W.J.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2002) Biochemistry & Biophysics, Univ. of Tokyo,
Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
REMARK Sequence update by submitter
COMMENT On May 16, 2002 this sequence version replaced AF250924.1.
FEATURES Location/Qualifiers
source 1..2182
/db_xref="H-InvDB:HIT000075030"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 42..2006
/note="peptide:N-glycanase; similar to yeast PNG1"
/codon_start=1
/product="PNGase"
/protein_id="AAF74720.2"
/translation="MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILR
NPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIR
DLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPP
SASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLD
KGINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAK
EVEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYT
DHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYS
CKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRIIVELVEFISPKTP
KPGELGGRISGSVAWRVARGEMGLQRKETLFIPCENEKISKQLHLCYNIVKDRYVRVS
NNNQTISGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKFECGSVGLKVDS
ISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSLHSYADFSGATEVILEAELSRGDGD
VAWQHTQLFRQSLNDHEENCLEIIIKFSDL"
BASE COUNT 653 a 423 c 528 g 578 t
ORIGIN
1 ggagagcgag gggcggcggc ggcgcccgct ggcgctcaag catggcggcg gcggcattgg
61 gcagctcctc aggctcggcg tccccggccg tggctgagct ctgccagaac accccggaga
121 cctttttgga ggcctccaag ctgctgctca cctatgctga caacatcctc agaaacccta
181 atgatgaaaa atatagatcc atccggattg gaaacacagc cttttctact agactcttgc
241 ctgtcagagg agctgttgaa tgtttatttg aaatgggctt tgaagaggga gaaacacatc
301 tcatctttcc taaaaaagct tcagtggagc agctgcaaaa aattcgtgac ctgattgcca
361 tagagagaag tagcagactg gatggctcaa ataagagcca caaagtaaag tcatctcagc
421 aacctgcagc cagtacccag cttcctacaa caccatcttc aaatcccagt gggttaaacc
481 agcacacaag gaaccgtcaa gggcagtcat cagatccacc atctgcttca acggttgctg
541 ctgactcagc cattctagaa gttcttcagt ccaacattca gcatgtgctg gtctatgaaa
601 atcctgctct tcaggagaaa gcgttggctt gtattccggt ccaagaacta aaaaggaaat
661 cacaagaaaa gttatcgaga gctagaaaat tggataaagg tatcaatata agtgatgagg
721 attttctttt gctggagctt ttgcactggt ttaaggaaga attttttcac tgggtgaata
781 acgttttgtg cagcaaatgt ggtggacaga ctaggtctag agatagatca ttactgccca
841 gtgatgatga gctgaagtgg ggtgcaaagg aagtggaaga tcattactgt gatgcctgcc
901 agttcagcaa tcgattccca agatataata accctgagaa acttttggaa acaagatgtg
961 gacggtgtgg cgagtgggcc aattgtttta cactgtgctg ccgagctgta gggtttgaag
1021 ctcgctatgt ttgggattac acagaccatg tctggacaga agtctattct ccttctcagc
1081 agcggtggct gcactgtgat gcatgtgaag atgtctgtga caagccactc ctttatgaaa
1141 taggatgggg caagaagctt tcctatgtca tagcattttc aaaagatgag gtagttgatg
1201 tcacttggcg atattcctgc aaacatgaag aggtgattgc cagaagaact aaggttaaag
1261 aagcattact tcgagacact attaatgggc ttaataagca gaggcaactg tttttgtcag
1321 aaaacagaag gaaagaactt ctccagagga taattgtgga gcttgttgaa tttatatctc
1381 ccaaaacccc taaacctgga gaacttgggg gaagaatatc tgggtcagtg gcttggagag
1441 tagcccgagg tgaaatgggt ctacagagaa aagaaacctt gtttattccc tgtgaaaatg
1501 agaagatttc taaacagctc cacctttgtt acaatattgt gaaagatcgt tatgttcgag
1561 tttcaaataa caatcaaacc atttctggat gggagaatgg cgtgtggaaa atggaatcta
1621 tattcagaaa agttgaaaca gactggcaca tggtatattt ggcccgaaag gaaggatcat
1681 cttttgctta tatttcctgg aagtttgagt gtgggtcagt tggcctaaaa gtagatagca
1741 tttctattag aacaagtagt caaacttttc agactggaac agtagaatgg aaattgcgat
1801 ctgatacagc acaagtagaa ctgacaggcg ataacagtct tcactcctat gctgattttt
1861 ctggtgccac tgaagttatt ttggaagcag aattaagcag aggagatggt gatgtcgctt
1921 ggcaacacac ccagctgttt agacaaagct taaatgacca tgaagaaaat tgtttggaga
1981 taattataaa attcagtgac ctttgagaac ctgaacatta tagaaaagct ggcaataatc
2041 aaggacttac tgaagtagtc tgttggttca gtgcatgctt agttggcagt taccaccctg
2101 tgctagcata tttcttttgc tagctatcca tcatgtaacc ctcatgaaaa ttatctttat
2161 acgtggacta taataaaata tt
//