LOCUS AF250847 2289 bp mRNA linear HUM 17-JUL-2000
DEFINITION Homo sapiens mitochondrial ceramidase mRNA, complete cds; nuclear
gene for mitochondrial product.
ACCESSION AF250847
VERSION AF250847.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2289)
AUTHORS El Bawab,S., Roddy,P., Qian,T., Bielawska,A., Lemasters,J.J. and
Hannun,Y.A.
TITLE Molecular cloning and characterization of a human mitochondrial
ceramidase
JOURNAL J. Biol. Chem. 275 (28), 21508-21513 (2000)
PUBMED 10781606
REFERENCE 2 (bases 1 to 2289)
AUTHORS El Bawab,S. and Hannun,Y.A.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2000) Biochemistry, Medical University of South
Carolina, 114 Doughty Street, P.O. Box 250780, Charleston, SC
29425, USA
FEATURES Location/Qualifiers
source 1..2289
/db_xref="H-InvDB:HIT000075025"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 1..2286
/function="cleaves lipid ceramide into free fatty acid and
sphingosine"
/note="active in neutral to alkaline range"
/codon_start=1
/product="mitochondrial ceramidase"
/protein_id="AAF86240.1"
/translation="MSAITVALLSLLFITSGTIENHKDLGGHFFSTTQSPPATQGSTA
AQRSTATQHSTATQSSTATQTSPVPLTPESPLFQNFSGYHIGVGRADCTGQVADINLM
GYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKY
GSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTN
MKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYPSNTDKEMIVLKMVDLNGDDLG
LISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDV
SPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKEL
YASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNF
TQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQII
TLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMTVVISGLCNVYTHYITTYEE
YQAQRYEAASTIYGPHALSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIP
SIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEA
TSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDI
LKPAVILSFEGTSPAFEVVTI"
BASE COUNT 664 a 553 c 515 g 557 t
ORIGIN
1 atgagtgcca tcacagtggc ccttctcagc ctcttgttta tcaccagtgg gaccattgaa
61 aaccacaaag atttaggagg ccattttttt tcaaccaccc aaagccctcc agccacccag
121 ggctccacag ctgcccaacg ctccacagcc acccagcatt ccacagccac ccagagctcc
181 acagccactc aaacttctcc agtgccttta accccagagt ctcctctatt tcagaacttc
241 agtggctacc atattggtgt tggacgagct gactgcacag gacaagtagc agatatcaat
301 ttgatgggct atggcaaatc cggccagaat gcacagggca tcctcaccag gctatacagt
361 cgtgccttca tcatggcaga acctgatggg tccaatcgaa cagtgtttgt cagcatcgac
421 ataggcatgg tatcacaaag gctcaggctg gaggtcctga acagactgca gagtaaatat
481 ggctccctgt acagaagaga taatgtcatc ctgagtggca ctcacactca ttcaggtcct
541 gcaggatatt tccagtatac cgtgtttgta attgccagtg aaggatttag caatcaaact
601 tttcagcaca tggtcactgg tatcttgaag agcattgaca tagcacacac aaatatgaaa
661 ccaggcaaaa tcttcatcaa taaaggaaat gtggatggtg tgcagatcaa cagaagtccg
721 tattcttacc ttcaaaatcc gcagtcagag agagcaaggt atccttcaaa tacagacaag
781 gaaatgatag ttttgaaaat ggtagatttg aatggagatg acttgggcct tatcagctgg
841 tttgccatcc acccggtcag catgaacaac agtaaccatc ttgtaaacag tgacaatgtg
901 ggctatgcat cttacctgct tgagcaagag aagaacaaag gatatctacc tggacagggg
961 ccatttgtag cagcctttgc ttcatcaaac ctaggagatg tgtcccccaa cattcttgga
1021 ccacgttgca tcaacacagg agagtcctgt gataacgcca atagcacttg tcccattggt
1081 gggcctagca tgtgcattgc taagggacct ggacaggata tgtttgacag cacacaaatt
1141 ataggacggg ccatgtatca gagagcaaag gaactctatg cctctgcctc ccaggaggta
1201 acaggaccac tggcttcagc acaccagtgg gtggatatga cagatgtgac tgtctggctc
1261 aattccacac atgcatcaaa aacatgtaaa ccagcattgg gctacagttt tgcggctggc
1321 actattgatg gagttggagg cctcaatttt acacagggga aaacagaagg ggatccattt
1381 tgggacacca ttcgggacca gatcctggga aagccatctg aagaaattaa agaatgtcat
1441 aaaccaaagc ccatccttct tcacaccgga gaactatcaa aacctcaccc ctggcatcca
1501 gacattgttg atgttcagat tattaccctt gggtccttgg ccataactgc catccccggg
1561 gagtttacga ccatgtctgg acgaagactt cgagaggcag ttcaagcaga atttgcatct
1621 catgggatgc agaacatgac tgttgttatt tcaggtctat gcaacgtcta tacacattac
1681 attaccactt atgaagaata ccaggctcag cgatatgagg cagcatcgac aatttatgga
1741 ccgcacgcat tatctgctta cattcagctc ttcagaaacc ttgctaaggc tattgctacg
1801 gacacggtag ccaacctgag cagaggtcca gaacctccct ttttcaaaca attaatagtt
1861 ccattaattc ctagtattgt ggatagagca ccaaaaggca gaactttcgg ggatgtcctg
1921 cagccagcaa aacctgaata cagagtgggg gaagttgctg aagttatatt tgtaggtgct
1981 aacccgaaga attcagtaca aaaccagacc catcagacct tcctcactgt ggagaaatat
2041 gaggctactt caacatcgtg gcagatagtg tgtaatgatg cctcctggga gactcgtttt
2101 tattggcaca agggactcct gggtctgagt aatgcaacag tggaatggca tattccagac
2161 actgcccagc ctggaatcta cagaataaga tattttggac acaatcggaa gcaggacatt
2221 ctgaagcctg ctgtcatact ttcatttgaa ggcacttccc cggcttttga agttgtaact
2281 atttagtga
//