LOCUS AF181972 5160 bp mRNA linear HUM 27-DEC-1999
DEFINITION Homo sapiens NADH/NADPH thyroid oxidase p138-tox mRNA, complete
cds.
ACCESSION AF181972
VERSION AF181972.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5160)
AUTHORS Dupuy,C., Ohayon,R., Valent,A., Noel-Hudson,M.S., Deme,D. and
Virion,A.
TITLE Purification of a novel flavoprotein involved in the thyroid NADPH
oxidase. Cloning of the porcine and human cdnas
JOURNAL J. Biol. Chem. 274 (52), 37265-37269 (1999)
PUBMED 10601291
REFERENCE 2 (bases 1 to 5160)
AUTHORS Dupuy,C., Ohayon,R., Valent,A., Noel-Hudson,M.S., Deme,D. and
Virion,A.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-1999) Unite 486, INSERM, 5 rue Jean-Baptiste
Clement, Chatenay-Malabry Cedex 92296, France
FEATURES Location/Qualifiers
source 1..5160
/db_xref="H-InvDB:HIT000072830"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 1..3633
/function="generates hydrogen peroxide by transfering
electrons from NADH/NADPH to molecular oxygen"
/note="calcium-dependent flavoprotein"
/codon_start=1
/product="NADH/NADPH thyroid oxidase p138-tox"
/protein_id="AAF20055.1"
/translation="MVPPGVYMRNASCHFRKVLNKGFQSSQALRVCNNYWIRENPNLN
STQEVNELLLGMASQISELEDNIVVEDLRDYWPGPGKFSRTDYVASSIQRGRDMGLPS
YSQALLAFGLDIPRNWSDLNPNVDPQVLEATAALYNQDLSQLELLLGGLLESHGDPGP
LFSAIVLDQFVRLRDGDRYWFENTRNGLFSKKEIEDIRNTTLRDVLVAVINIDPSALQ
PNVFVWHKGAPCPQPKQLTTDGLPQCAPLTVLDFFEGSSPGFAITIIALCCLPLVSLL
LSGVVAYFRGREHKKLQKKLKESVKKEAAKDGVPAMEWPGPKERSSPIIIQLLSDRCL
QVLNRHLTVLRVVQLQPLQQVNLILSNNRGCRTLLLKIPKEYDLVLLFSSEEERGAFV
QQLWDFCVRWALGLHVAEMSEKELFRKAVTKQQRERILEIFFRHLFAQVLDINQADAG
TLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLD
ILVVFMKGSPEDKSRLMFTMYDLDENGFLSKDEFFTMMRSFIEISNNCLSKAQLAEVV
ESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVKGGGGGGNGIRDIFKQNISC
RVSFITRTPGERSHPQGLGPPAPEAPELGGPGLKKRFGKKAAVPTPRLYTEALQEKMQ
RGFLAQKLQQYKRFVENYRRHIVCVAIFSAICVGVFADRAYYYGFALPPSDIAQTTLV
GIILSRGTAASVSFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRWIAMAAVV
LAILHSAGHAVNVYIFSVSPLSLLACIFPNVFVNDGSKLPQKFYWWFFQTVPGMTGVL
LLLVLAIMYVFASHHFRRRSFRGFWLTHHLYILLYALLIIHGSYALIQLPTFHIYFLV
PAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTYLQFQRPQGFEYKSGQWVRIACLAL
GTTEYHPFTLTSAPHEDTLSLHIRAVGPWTTRLREIYSSPKGNGCAGYPKLYLDGPFG
EGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSLGSQMLCKKIYFIWVTRTQRQF
EWLADIIQEVEENDHQDLVSVHIYVTQLAEKFDLRTTMLYICERHFQKVLNRSLFTGL
RSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLVNRQDRAHFM
HHYENF"
misc_feature 28..30
/note="glycosylation site"
misc_feature 130..132
/note="glycosylation site"
misc_feature 349..351
/note="glycosylation site"
misc_feature 595..597
/note="glycosylation site"
misc_feature 1480..1518
/note="Region: EF-Hand motif"
misc_feature 1588..1626
/note="Region: EF-Hand motif"
misc_feature 2365..2367
/inference="non-experimental evidence, no additional
details recorded"
/note="Region: heme binding"
misc_feature 2407..2409
/inference="non-experimental evidence, no additional
details recorded"
/note="Region: heme binding"
misc_feature 2650..2652
/inference="non-experimental evidence, no additional
details recorded"
/note="Region: heme binding"
misc_feature 2689..2691
/inference="non-experimental evidence, no additional
details recorded"
/note="Region: heme binding"
misc_feature 2932..2952
/note="Region: isoalloxazine FAD-binding motif"
misc_feature 3142..3162
/note="Region: ribose NADPH-binding motif"
misc_feature 3520..3543
/note="Region: adenine NADPH-binding motif"
BASE COUNT 1151 a 1482 c 1306 g 1221 t
ORIGIN
1 atggtgcccc ctggtgtcta catgagaaat gccagctgtc atttccggaa ggtcctgaac
61 aagggttttc aaagctccca agctctcagg gtctgcaaca actactggat tcgggagaac
121 cccaatctga acagtaccca ggaggtgaat gagctgctgc tgggaatggc ctcccagatt
181 tcggagttgg aggacaacat agtggttgaa gatctgaggg attactggcc tggccctggc
241 aaattctccc gtacagacta tgtggccagc agcatccaac gtggccgaga tatggggctg
301 cccagctata gccaggccct gctggccttt gggctggaca tcccaaggaa ctggagtgat
361 ctcaacccta atgtggaccc ccaggtgctg gaggccacag ctgccctgta caaccaggac
421 ctatcccagc tagagctgct ccttggcggg ctcctggaga gccatgggga ccctggaccc
481 ctgttcagtg ccattgtcct cgaccagttt gtacggctgc gggatggtga ccgctactgg
541 tttgagaaca ccaggaatgg gctgttctcc aagaaggaga ttgaagacat ccgaaatacc
601 accctgcggg acgtgctggt cgctgttatc aacattgacc ccagtgccct gcagcccaat
661 gtctttgtct ggcataaagg tgcaccctgc cctcaaccta agcagctcac aactgacggc
721 ctgccccagt gtgcacccct gactgtgctt gacttctttg aaggcagcag ccctggtttt
781 gccatcacca tcattgctct ctgctgcctt cccttagtga gtctgcttct ctctggagtg
841 gtggcctatt tccggggccg agaacacaag aagctacaaa agaaactcaa agagagcgtg
901 aagaaggaag cagccaaaga tggagtgcca gcgatggagt ggccaggccc caaggagagg
961 agcagtccca tcatcatcca gctgctgtca gacaggtgtc tgcaggtcct gaacaggcat
1021 ctcactgtgc tccgtgtggt ccagctgcag cctctgcagc aggtcaacct catcctgtcc
1081 aacaaccgag gatgccgcac cctgctgctc aagatcccta aggagtatga cctggtgctg
1141 ctgtttagtt ctgaagagga acggggcgcc tttgtgcagc agctatggga cttctgcgtg
1201 cgctgggctc tgggcctcca tgtggctgag atgagcgaga aggagctatt taggaaggct
1261 gtgacaaagc agcagcggga acgcatcctg gagatcttct tcagacacct ttttgctcag
1321 gtgctggaca tcaaccaggc cgacgcaggg accctgcccc tggactcctc ccagaaggtg
1381 cgggaggccc tgacctgcga gctgagcagg gccgagtttg ccgagtccct gggcctcaag
1441 ccccaggaca tgtttgtgga gtccatgttc tctctggctg acaaggatgg caatggctac
1501 ctgtccttcc gagagttcct ggacatcctg gtggtcttca tgaaaggctc cccagaggat
1561 aagtcccgtc taatgtttac catgtatgac ctggatgaga atggcttcct ctccaaggac
1621 gaattcttca ccatgatgcg atccttcatc gagatctcca acaactgcct gtccaaggcc
1681 caactggccg aggtggtgga gtctatgttc cgggagtcgg gattccagga caaggaggag
1741 ctgacatggg aggattttca cttcatgctg cgggaccatg acagcgagct ccgcttcacg
1801 cagctctgtg tcaaaggtgg aggtggaggt ggaaatggta ttagagatat ctttaaacaa
1861 aacatcagct gtcgagtctc gttcatcact cggacacctg gggagcgctc ccacccccag
1921 ggactggggc cccctgcccc agaagcccca gagctgggag gccctggact gaagaagagg
1981 tttggcaaaa aggcagcagt gcccactccc cggctgtaca cagaggcgct gcaagagaag
2041 atgcagcgag gcttcctagc ccaaaagctg cagcagtaca agcgcttcgt ggagaactac
2101 cggaggcaca tcgtgtgtgt ggcaatcttc tcggccatct gtgttggcgt gtttgcagat
2161 cgtgcttact actatggctt tgccttgcca ccctcggaca ttgcacagac caccctcgtg
2221 ggcatcatcc tgtcacgagg cacggcggcc agcgtctcct tcatgttctc ttatatcttg
2281 ctcaccatgt gccgcaacct cataaccttc ctgcgagaga ctttcctcaa ccgctatgtg
2341 ccttttgatg ccgcagtgga cttccaccgc tggatcgcca tggctgctgt tgtcctggcc
2401 attttgcaca gtgctggcca cgcagtcaat gtctacatct tctcagtcag cccactcagc
2461 ctgctggcct gcatattccc caacgtcttt gtgaatgatg ggtccaagct tccccagaag
2521 ttctattggt ggttcttcca gaccgtccca ggtatgacag gtgtgcttct gctcctggtc
2581 ctggccatca tgtatgtctt cgcctcccac cacttccgcc gccgcagctt ccggggcttc
2641 tggctgaccc accacctcta catcctgctc tatgccctgc tcatcatcca tggcagctat
2701 gctctgatcc agctgcccac tttccacatc tacttcctgg tcccggcaat catctatgga
2761 ggtgacaagc tggtgagcct gagccggaag aaggtggaga tcagcgtggt gaaggcggag
2821 ctgctgccct caggagtgac ctacctgcaa ttccagaggc cccaaggctt tgagtacaag
2881 tcaggacagt gggtgcggat cgcctgcctg gctctgggga ccaccgagta ccaccccttc
2941 acactgacct ccgcgcccca tgaggacaca ctcagcctgc acatccgggc agtggggccc
3001 tggaccactc gcctcaggga gatctactca tccccaaagg gcaatggctg tgctggatac
3061 ccaaagctgt accttgatgg accgtttgga gagggccatc aggagtggca taaatttgag
3121 gtgtcagtgt tggtgggagg gggcattggg gtcaccccct ttgcctccat cctcaaagac
3181 ctggtcttca agtcatcctt gggcagccaa atgctgtgta agaagatcta cttcatctgg
3241 gtgacacgga cccagcgtca gtttgagtgg ctggctgaca tcatccaaga ggtggaggag
3301 aacgaccacc aggacctggt gtctgtgcac atttatgtca cccagctggc tgagaagttc
3361 gacctcagga ccaccatgct atacatctgc gagcggcact tccagaaagt gctgaaccgg
3421 agtctgttca cgggcctgcg ctccatcacc cactttggcc gtcccccctt cgagcccttc
3481 ttcaactccc tgcaggaggt ccacccacag gtgcgcaaga tcggggtgtt cagctgcggc
3541 cctccaggaa tgaccaagaa tgtagagaag gcctgtcagc tcgtcaacag gcaggaccga
3601 gcccacttca tgcaccacta tgagaacttc tgagcctgtc ctccctggct gctgcttcca
3661 gtatcctgcc ttctcttctg tgcacctaag ttgcccagcc ctgctggcaa tctctccatc
3721 agaatccacc ttaggcctca gctggagggc tgcagagccc ctcccaatat tgggagaata
3781 ttgacccaga caattataca aatgagaaaa ggcaggagac tatgttctac aattgcagtg
3841 catgatgatt ataagtccac ctgtttatca acggcaccat tcctgcagcc ctccagactt
3901 cctgccctta gcaagtgcgc aaccagtcag gatctcccaa agaagataaa gaccactcct
3961 caccccagct caagccatgg caggcgtggc aagcaaagtg gggaggagac agtccctgct
4021 tgtgacaagt gtggaggtga aaaggtacaa tagtgcttgt ctccgatagc tccccacatc
4081 tctaattgac ttccacaaaa tcgatgcgtt gctttggtat ttgcttggac tgacatttga
4141 gggaggagga ggctgggatc ctctggctga gaatctcctc agagcccagt gcagaagctg
4201 tgatgcttag aacctggaca gcccgactgc ctcaactctg tctccaggtc tattccctcc
4261 agctccaaaa ggagcagccc tacttctacc ccttcccgtc cccaaagtgt cagcaacttt
4321 gaggagggca ccaggaaaca aagatgcctc cccagccctg atattcttga tgtcaccagt
4381 gatacccact gccctgaccc ctgggcaggc ccctctccgc atctactgga gtggtccctg
4441 ggctctgggg ctgaaggatt ccagcctctc tgccagatat tcagtactcg atctcaattc
4501 ccctcttcca caagagttgg gtgaccagct gtcctagttt gcccaggact ctccctgttt
4561 tagcactgaa agtctcttgc cccaggaaac cccatcagtc ccaggcagat tgggacagct
4621 ggtcacctta cgcaagagcc aggctgaaac atcccctcca tactcagctc tttaactttt
4681 cttttccttt ttcatcgggc tctttcctaa aaagctgagc tgtaaaatat tttacatcga
4741 ggtataataa ataatcatgt acatgtttta ccaccaccca ggtcaagaca tagaatgttt
4801 caacatttcc atcaccccag aaactcccct tgtaccccct tccacttcgt ctcccctagc
4861 tcctagaagc aaccactgat gtgatttcta ccaaatccag ttttggtcct actaaatata
4921 ctcttttgag actggcctct tttactcacc ataatgcctt tgtaattcat ccatgctgtt
4981 gtgtgtatca gcagtttgtt ccttttcatt gctgagtagt attctattgt agagatgtac
5041 cacagtttgt ttattcttct gttgatggac gtttgggttg tttctaattt tgaatgatta
5101 taaataaaaa ttctgtgagt gttcttgtac gtaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//