LOCUS AF144074 2917 bp mRNA linear HUM 01-MAY-2000
DEFINITION Homo sapiens glucosidase II alpha subunit mRNA, complete cds.
ACCESSION AF144074
VERSION AF144074.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2917)
AUTHORS Marcil,A., Sevigny,G. and Thomas,D.Y.
TITLE Expression of Enzymatically Active Isoforms of Human Glucosidase II
in Insect Cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2917)
AUTHORS Marcil,A., Sevigny,G. and Thomas,D.Y.
TITLE Direct Submission
JOURNAL Submitted (19-APR-1999) Eucaryotic Genetic, Biotechnology Research
Institute, 6100 Ave Royalmount, Montreal, Quebec H4P 2R2, Canada
FEATURES Location/Qualifiers
source 1..2917
/db_xref="H-InvDB:HIT000071272"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="11"
/map="11q12-q13"
/cell_line="HeLa"
CDS 17..2917
/note="similar to sequences encoded by GenBank Accession
Numbers D42041 and U92793"
/codon_start=1
/product="glucosidase II alpha subunit"
/protein_id="AAF66685.1"
/translation="MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEES
SFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFR
IDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDL
LEDRSLLLSVNARGLLEFEHQRAPRVSFSDKVNLTLGSIWDKIKNLFSRQGSKDPAEG
DGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGME
HVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLG
IFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGP
SISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWL
DIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNL
GLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMN
EPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLAR
AFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELL
VRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQA
HREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVW
YDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVAL
SPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERV
VIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR"
BASE COUNT 622 a 766 c 829 g 700 t
ORIGIN
1 gcaaactctg cacaagatgg cggcggtagc ggcagtggcg gcgcgtagga ggcggtcttg
61 ggcgtctttg gtactggctt ttttaggggt ctgcctgggg attacccttg ctgtggatag
121 aagcaacttt aagacctgtg aagagagttc tttctgcaag cgacagagaa gcatacggcc
181 aggcctctct ccataccgag ccttgctgga ctctctacag cttggtcctg attccctcac
241 ggtccatctg atccatgagg tcaccaaggt gttgctggtg ctagagcttc aggggcttca
301 aaagaacatg actcggttca ggattgatga gctggagcct cggcgacccc gataccgtgt
361 accagatgtt ttggtggctg atccaccaat agcccggctt tctgtctctg gtcgtgatga
421 gaacagtgtg gagttaacca tggctgaggg accctacaag atcatcttga cagcacggcc
481 attccgcctt gacctactag aggaccgaag tcttttgctt agtgtcaatg cccgaggact
541 cttggagttt gagcatcaga gggcccctag ggtctctttc tcggataagg ttaatctcac
601 gcttggtagc atatgggata agatcaagaa ccttttctct aggcaaggat caaaagaccc
661 agctgagggc gatggggccc agcctgagga aacacccagg gatggcgaca agccagagga
721 gactcagggg aaggcagaga aagatgagcc aggagcctgg gaggagacat tcaaaactca
781 ctctgacagc aagccgtatg gccccatgtc tgtgggtttg gacttctctc tgccaggcat
841 ggagcatgtc tatgggatcc ctgagcatgc agacaacctg aggctgaagg tcactgaggg
901 tggggagcca tatcgcctct acaatttgga tgtgttccag tatgagctgt acaacccaat
961 ggccttgtat gggtctgtgc ctgtgctcct ggcacacaac cctcatcgcg acttgggcat
1021 cttctggctc aatgctgcag agacctgggt tgatatatct tccaacactg ccgggaagac
1081 cctgtttggg aagatgatgg actacctgca gggctctggg gagaccccac agacagatgt
1141 tcgctggatg tcagagactg gcatcattga cgtcttcctg ctgctggggc cctccatctc
1201 tgatgttttc cggcaatatg ctagtctcac aggaacccag gcgttgcccc cactcttctc
1261 cctcggctac caccagagcc gttggaacta ccgggacgag gctgatgtgc tggaagtgga
1321 tcagggcttt gatgatcaca acctgccctg tgatgtcatc tggctagaca ttgaacatgc
1381 tgatggcaag cggtatttca cctgggaccc cagtcgcttc cctcagcccc gcaccatgct
1441 tgagcgcttg gcttctaaga ggcggaagct ggtggccatc gtagaccccc acatcaaggt
1501 ggactccggc taccgagttc acgaggagct gcggaacctg gggctgtatg ttaaaacccg
1561 ggatggctct gactatgagg gctggtgctg gccaggctca gctggttacc ctgacttcac
1621 taatcccacg atgagggcct ggtgggctaa catgttcagc tatgacaatt atgagggctc
1681 agctcccaac ctctttgtct ggaatgacat gaacgaacca tctgtgttca atggtcctga
1741 ggtcaccatg ctcaaggatg cccagcatta tgggggctgg gagcaccggg atgtgcataa
1801 catctatggc ctttatgtgc acatggcgac tgctgatggg ctgagacagc gctctggggg
1861 catggaacgc ccctttgtcc tggccagggc cttcttcgct ggctcccagc gctttggagc
1921 cgtgtggaca ggggacaaca ctgccgagtg ggaccatttg aagatctcta ttcctatgtg
1981 tctcagcttg gggctggtgg gactttcctt ctgtggggcg gatgtgggtg gcttcttcaa
2041 aaacccagag ccagagctgc ttgtgcgctg gtaccagatg ggtgcttacc agccattctt
2101 ccgggcacat gcccacttgg acactgggcg acgagagcca tggctgttac catctcagca
2161 caatgatata atccgagatg ccttgggcca gcgatattct ttgctgccct tctggtacac
2221 cctcttatat caggcccatc gggaaggcat tcctgtcatg agacccctgt gggtgcagta
2281 ccctcaggat gtgactacct tcaatataga tgatcagtac ttgcttgggg atgcgttgct
2341 ggttcaccct gtatcagact ctggagccca tggtgtccag gtctatctgc ctggccaagg
2401 ggaggtgtgg tatgacattc aaagctacca gaagcatcat ggtccccaga ccctgtacct
2461 gcctgtaact ctaagcagta tccctgtgtt ccagcgtgga gggacaatcg tgcctcgatg
2521 gatgcgagtg cggcggtctt cagaatgtat gaaggatgac cccatcactc tctttgttgc
2581 acttagccct cagggtacag ctcaaggaga gctctttctg gatgatgggc acacgttcaa
2641 ctatcagact cgccaagagt tcctgctgcg tcgattctca ttctctggca acacccttgt
2701 ctccagctca gcagaccctg aaggacactt tgagacacca atctggattg agcgggtggt
2761 gataataggg gctggaaagc cagcagctgt ggtactccag acaaaaggat ctccagaaag
2821 ccgcctgtcc ttccagcatg accctgagac ctctgtgttg gtcctgcgca agcctggcat
2881 caatgtggca tctgattgga gtattcacct gcgataa
//