LOCUS AF105374 1968 bp mRNA linear HUM 11-JUN-2001
DEFINITION Homo sapiens heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2
(3OST2) mRNA, 3OST2-1 allele, complete cds.
ACCESSION AF105374
VERSION AF105374.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1968)
AUTHORS Shworak,N.W., Liu,J., Petros,L.M., Zhang,L., Kobayashi,M.,
Copeland,N.G., Jenkins,N.A. and Rosenberg,R.D.
TITLE Multiple isoforms of heparan sulfate D-glucosaminyl
3-O-sulfotransferase. Isolation, characterization, and expression
of human cdnas and identification of distinct genomic loci
JOURNAL J. Biol. Chem. 274 (8), 5170-5184 (1999)
PUBMED 9988767
REFERENCE 2 (bases 1 to 1968)
AUTHORS Shworak,N.W., Liu,J. and Rosenberg,R.D.
TITLE Direct Submission
JOURNAL Submitted (12-NOV-1998) Biology, Massachusetts Institute of
Technology, 31 Ames St, Cambridge, MA 02139, USA
FEATURES Location/Qualifiers
source 1..1968
/db_xref="H-InvDB:HIT000069482"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="16"
/map="16p12"
/tissue_type="brain"
/note="isolated by hybridization screening using IMAGE
Consortium clone c-20d10 found in GenBank Accession Number
F07258"
gene 1..1968
/gene="3OST2"
/allele="3OST2-1"
5'UTR <1..72
/gene="3OST2"
/allele="3OST2-1"
CDS 73..1176
/gene="3OST2"
/allele="3OST2-1"
/EC_number="2.8.2.23"
/note="3-OST-2"
/codon_start=1
/product="heparan sulfate D-glucosaminyl
3-O-sulfotransferase-2"
/protein_id="AAD30206.1"
/translation="MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDL
GRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSN
HSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDW
YRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQT
LSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGER
LITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTH
VQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE"
misc_feature 73..129
/gene="3OST2"
/allele="3OST2-1"
/note="cytoplasmic domain; other site"
misc_feature 130..195
/gene="3OST2"
/allele="3OST2-1"
/note="transmembrane region"
misc_feature 196..399
/gene="3OST2"
/allele="3OST2-1"
/note="SPLAG domain; Ser, Pro, Leu, Ala, Gly enriched;
other site"
misc_feature 235..291
/gene="3OST2"
/allele="3OST2-1"
/note="putative cystine bridged peptide loop; other site"
misc_feature 307..309
/gene="3OST2"
/allele="3OST2-1"
/note="putative O-glycosylation site"
misc_feature 313..315
/gene="3OST2"
/allele="3OST2-1"
/note="putative O-glycosylation site"
misc_feature 322..324
/gene="3OST2"
/allele="3OST2-1"
/note="putative O-glycosylation site"
misc_feature 331..333
/gene="3OST2"
/allele="3OST2-1"
/note="putative O-glycosylation site"
misc_feature 367..375
/gene="3OST2"
/allele="3OST2-1"
/note="putative N-glycosylation site"
misc_feature 400..1173
/gene="3OST2"
/allele="3OST2-1"
/note="sulfotransferase domain; other site"
misc_feature 424..462
/gene="3OST2"
/allele="3OST2-1"
/note="putative beta-strand/P-loop/alpha-helix motif;
binds 5'-phosphate of 3'-phosphoadenosine 5'-phosphate,
PAPS; binding site"
misc_feature 649..657
/gene="3OST2"
/allele="3OST2-1"
/note="putative N-glycosylation site"
misc_feature 667..714
/gene="3OST2"
/allele="3OST2-1"
/note="putative sheet-loop-helix structure for binding the
3'-phosphate of PAPS"
misc_feature 730..732
/gene="3OST2"
/allele="3OST2-1"
/note="putative O-glycosylation site"
misc_feature 775..783
/gene="3OST2"
/allele="3OST2-1"
/note="putative N-glycosylation site"
misc_feature 823..852
/gene="3OST2"
/allele="3OST2-1"
/note="putative structure for binding 5'-sulfate of PAPS"
misc_feature 988..996
/gene="3OST2"
/allele="3OST2-1"
/note="putative N-glycosylation site"
misc_feature 1009..1047
/gene="3OST2"
/allele="3OST2-1"
/note="putative cystine bridged peptide loop; other site"
misc_feature 1102..1110
/gene="3OST2"
/allele="3OST2-1"
/note="putative N-glycosylation site"
variation 804
/gene="3OST2"
/note="3OST2-2 allele; silent wobble position mutation"
/replace="a"
3'UTR 1177..1950
/gene="3OST2"
/allele="3OST2-1"
variation 1249
/gene="3OST2"
/note="3OST2-2 allele"
/replace="g"
variation 1350
/gene="3OST2"
/note="3OST2-2 allele"
/replace="c"
variation 1507
/gene="3OST2"
/note="3OST2-2 allele"
/replace="t"
regulatory 1743..1748
/regulatory_class="polyA_signal_sequence"
/gene="3OST2"
/allele="3OST2-1"
/note="putative"
regulatory 1925..1930
/regulatory_class="polyA_signal_sequence"
/gene="3OST2"
/allele="3OST2-1"
polyA_site 1950
/gene="3OST2"
/allele="3OST2-1"
BASE COUNT 441 a 603 c 490 g 434 t
ORIGIN
1 cgcagggcca cagcagctca gccgccggtg ccccctcgga aaccatgacc cccggcgcgg
61 gcccatggag ccatggccta tagggtcctg ggccgcgcgg ggccacctca gccgcggagg
121 gcgcgcaggc tgctcttcgc cttcacgctc tcgctctcct gcacttacct gtgttacagc
181 ttcctgtgct gctgcgacga cctgggtcgg agccgcctcc tcggcgcgcc tcgctgcctc
241 cgcggcccca gcgcgggcgg ccagaaactt ctccagaagt cccgcccctg tgatccctcc
301 gggccgacgc ccagcgagcc cagcgctccc agcgcgcccg ccgccgccgt gcccgcccct
361 cgcctctccg gttccaacca ctccggctca cccaagctgg gtaccaagcg gttgccccaa
421 gccctcattg tgggcgtgaa gaaggggggc acccgggccg tgctggagtt tatccgagta
481 cacccggacg tgcgggcctt gggcacggaa ccccacttct ttgacaggaa ctacggccgc
541 gggctggatt ggtacaggag cctgatgccc aggaccctcg agagccagat cacgctggag
601 aagacgccca gctactttgt cactcaagag gctcctcgac gcatcttcaa catgtcccga
661 gacaccaagc tgatcgtggt tgtgcggaac cctgtgaccc gtgccatctc tgattacacg
721 cagacactct ccaagaagcc cgacatcccg acctttgagg gcctctcctt ccgcaaccgc
781 accctgggcc tggtggacgt gtcgtggaac gccatccgca tcggcatgta cgtgctgcac
841 ctggagagct ggctgcagta cttcccgcta gctcagattc acttcgtcag tggcgagcga
901 ctcatcactg acccggccgg cgagatgggg cgagtccagg acttcctggg cattaagaga
961 ttcatcacgg acaagcactt ctatttcaac aagaccaaag gattcccttg cttgaaaaaa
1021 acagaatcga gcctcctgcc tcgatgcttg ggcaaatcaa aagggagaac tcatgtacag
1081 attgatcctg aagtgataga ccagctccga gaattttata gaccgtataa tatcaaattt
1141 tatgaaaccg ttgggcagga cttcaggtgg gaataagccc acgaaaggaa agggctctca
1201 agggctcttc tgctcatctc ttccgtgaga tttgctccca gaccctctta tctccctcca
1261 acaaaccctg gctccagccc cctttcccaa cttgagttgc atcatcttgg aaccaggaag
1321 cccagctaaa gccaagagac cagagagtct ctgccactag ttttcatcag tctgttcaag
1381 caaagttgat ctgctcctgg cacgtccagt aaattccaga atcattctcc tttctgccca
1441 taaagggcct tggagaattg ctttaagaag agtgaatgtt ccaatgatga tagatattat
1501 aagcgacgat ggttctgttg ctatgaacac agcagtcggt ccctgtcatt gtccacccag
1561 gagtggcctt gttaattcca agtggcatgt atcttccctc tgagcttcat ttcttcaaga
1621 tgctctgggt ggtgggatgg gagaccatcc tcagccctcc tcagacctta tcaattcatt
1681 gagagattgc aaagctgaaa gcacctccgg ccactcctgg gagacagacc ctttggtgat
1741 gaaataaacc agtgacttca gagcctatgg tctcaactgt gcttgaaaaa cactgtctct
1801 gaaaacaact ttgtgattct ccctgctccc tgtggacaaa agcacataat tctgctgtta
1861 cgggtacttt gctcatacga gctttcatgt tcagcatgca atggaatcat gcttgtccat
1921 gtgaaataaa tatggctctc tcgtgtcctt aaaaaaaaaa aaaaaaaa
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