LOCUS AF098951 2418 bp mRNA linear HUM 24-JAN-2001
DEFINITION Homo sapiens breast cancer resistance protein (BCRP) mRNA, complete
cds.
ACCESSION AF098951
VERSION AF098951.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2418)
AUTHORS Doyle,L.A., Yang,W., Abruzzo,L.V., Krogmann,T., Gao,Y., Rishi,A.K.
and Ross,D.D.
TITLE A multidrug resistance transporter from human MCF-7 breast cancer
cells
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 95 (26), 15665-15670 (1998)
PUBMED 9861027
REFERENCE 2 (bases 1 to 2418)
AUTHORS Ross,D.D., Yang,W., Abruzzo,L.V., Dalton,W.S., Schneider,E.,
Lage,H., Dietel,M., Greenberger,L., Cole,S.P. and Doyle,L.A.
TITLE Atypical multidrug resistance: breast cancer resistance protein
messenger RNA expression in mitoxantrone-selected cell lines
JOURNAL J. Natl. Cancer Inst. 91 (5), 429-433 (1999)
PUBMED 10070941
REFERENCE 3 (bases 1 to 2418)
AUTHORS Doyle,L.A., Yang,W., Abruzzo,L.V., Krogmann,T., Gao,Y., Rishi,A.K.
and Ross,D.D.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-1998) Greenebaum Cancer Center, Program of
Oncology, University of Maryland School of Medicine and Baltimore
VA Medical Center, 655 West Baltimore Street, BRB Rm 9-015,
Baltimore, MD 21201, USA
COMMENT On Jan 24, 2001 this sequence version replaced AF098951.1.
FEATURES Location/Qualifiers
source 1..2418
/db_xref="H-InvDB:HIT000068567"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="4"
/map="4q"
/cell_line="MCF-7/AdrVp"
/tissue_type="breast cancer"
/note="isolated from atypical multidrug resistant cells"
gene 1..2418
/gene="BCRP"
CDS 239..2206
/gene="BCRP"
/inference="non-experimental evidence, no additional
details recorded"
/note="ATP-binding cassette transporter; ABC superfamily
member; when expression of BCRP is enforced by
transfection of the cDNA in drug-sensitive human breast
cancer MCF-7 cells, the cloned transfected cells become
resistant to mitoxantrone, daunorubicin and doxorubicin,
display diminished intracellular accumulation of
daunorubicin, and manifest an ATP-dependent increase in
the efflux of Rhodamine 123 compared to vector transfected
controls"
/codon_start=1
/product="breast cancer resistance protein"
/protein_id="AAC97367.1"
/translation="MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICY
RVKLKSGFLPCRKPVEKEILSNINGIMKPGLNAILGPTGGGKSSLLDVLAARKDPSGL
SGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLATTMTNHEKNER
INRVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIGMELITDPSILFLDEPTTGLDS
STANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPAQEALGY
FESAGYHCEAYNNPADFFLDIINGDSTAVALNREEDFKATEIIEPSKQDKPLIEKLAE
IYVNSSFYKETKAELHQLSGGEKKKKITVFKEISYTTSFCHQLRWVSKRSFKNLLGNP
QASIAQIIVTVVLGLVIGAIYFGLKNDSTGIQNRAGVLFFLTTNQCFSSVSAVELFVV
EKKLFIHEYISGYYRVSSYFLGKLLSDLLPMTMLPSIIFTCIVYFMLGLKPKADAFFV
MMFTLMMVAYSASSMALAIAAGQSVVSVATLLMTICFVFMMIFSGLLVNLTTIASWLS
WLQYFSIPRYGFTALQHNEFLGQNFCPGLNATGNNPCNYATCTGEEYLVKQGIDLSPW
GLWKNHVALACMIVIFLTIAYLKLLFLKKYS"
BASE COUNT 692 a 506 c 521 g 699 t
ORIGIN
1 gggaggaggc agcctgtgga ggaactgggt aggatttagg aacgcaccgt gcacatgctt
61 ggtggtcttg ttaagtggaa actgctgctt tagagtttgt ttggaaggtc cgggtgactc
121 atcccaacat ttacatcctt aattgttaaa gcgctgcctc cgagcgcacg catcctgaga
181 tcctgagcct ttggttaaga ccgagctcta ttaagctgaa aagataaaaa ctctccagat
241 gtcttccagt aatgtcgaag tttttatccc agtgtcacaa ggaaacacca atggcttccc
301 cgcgacagct tccaatgacc tgaaggcatt tactgaagga gctgtgttaa gttttcataa
361 catctgctat cgagtaaaac tgaagagtgg ctttctacct tgtcgaaaac cagttgagaa
421 agaaatatta tcgaatatca atgggatcat gaaacctggt ctcaacgcca tcctgggacc
481 cacaggtgga ggcaaatctt cgttattaga tgtcttagct gcaaggaaag atccaagtgg
541 attatctgga gatgttctga taaatggagc accgcgacct gccaatttca aatgtaattc
601 aggttacgtg gtacaagatg atgttgtgat gggcactctg acggtgagag aaaacttaca
661 gttctcagca gctcttcggc ttgcaacaac tatgacgaat catgaaaaaa acgaacggat
721 taacagggtc attcaagagt taggtctgga taaagtggca gactccaagg ttggaactca
781 gtttatccgt ggtgtgtctg gaggagaaag aaaaaggact agtataggaa tggagcttat
841 cactgatcct tccatcttgt tcttggatga gcctacaact ggcttagact caagcacagc
901 aaatgctgtc cttttgctcc tgaaaaggat gtctaagcag ggacgaacaa tcatcttctc
961 cattcatcag cctcgatatt ccatcttcaa gttgtttgat agcctcacct tattggcctc
1021 aggaagactt atgttccacg ggcctgctca ggaggccttg ggatactttg aatcagctgg
1081 ttatcactgt gaggcctata ataaccctgc agacttcttc ttggacatca ttaatggaga
1141 ttccactgct gtggcattaa acagagaaga agactttaaa gccacagaga tcatagagcc
1201 ttccaagcag gataagccac tcatagaaaa attagcggag atttatgtca actcctcctt
1261 ctacaaagag acaaaagctg aattacatca actttccggg ggtgagaaga agaagaagat
1321 cacagtcttc aaggagatca gctacaccac ctccttctgt catcaactca gatgggtttc
1381 caagcgttca ttcaaaaact tgctgggtaa tccccaggcc tctatagctc agatcattgt
1441 cacagtcgta ctgggactgg ttataggtgc catttacttt gggctaaaaa atgattctac
1501 tggaatccag aacagagctg gggttctctt cttcctgacg accaaccagt gtttcagcag
1561 tgtttcagcc gtggaactct ttgtggtaga gaagaagctc ttcatacatg aatacatcag
1621 cggatactac agagtgtcat cttatttcct tggaaaactg ttatctgatt tattacccat
1681 gacgatgtta ccaagtatta tatttacctg tatagtgtac ttcatgttag gattgaagcc
1741 aaaggcagat gccttcttcg ttatgatgtt tacccttatg atggtggctt attcagccag
1801 ttccatggca ctggccatag cagcaggtca gagtgtggtt tctgtagcaa cacttctcat
1861 gaccatctgt tttgtgttta tgatgatttt ttcaggtctg ttggtcaatc tcacaaccat
1921 tgcatcttgg ctgtcatggc ttcagtactt cagcattcca cgatatggat ttacggcttt
1981 gcagcataat gaatttttgg gacaaaactt ctgcccagga ctcaatgcaa caggaaacaa
2041 tccttgtaac tatgcaacat gtactggcga agaatatttg gtaaagcagg gcatcgatct
2101 ctcaccctgg ggcttgtgga agaatcacgt ggccttggct tgtatgattg ttattttcct
2161 cacaattgcc tacctgaaat tgttatttct taaaaaatat tcttaaattt ccccttaatt
2221 cagtatgatt tatcctcaca taaaaaagaa gcactttgat tgaagtattc aatcaagttt
2281 ttttgttgtt ttctgttccc ttgccatcac actgttgcac agcagcaatt gttttaaaga
2341 gatacatttt tagaaatcac aacaaactga attaaacatg aaagaaccca aaaaaaaaga
2401 tatcactcag cataatga
//