LOCUS AF083106 4086 bp mRNA linear HUM 14-APR-2000
DEFINITION Homo sapiens sirtuin type 1 (SIRT1) mRNA, complete cds.
ACCESSION AF083106
VERSION AF083106.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4086)
AUTHORS Frye,R.A.
TITLE Characterization of five human cDNAs with homology to the yeast
SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may
have protein ADP-ribosyltransferase activity
JOURNAL Biochem. Biophys. Res. Commun. 260 (1), 273-279 (1999)
PUBMED 10381378
REFERENCE 2 (bases 1 to 4086)
AUTHORS Frye,R.A.
TITLE Direct Submission
JOURNAL Submitted (10-AUG-1998) Pathology, VA Med. Cent. (132L), Univ. of
Pittsburgh, University Drive C, Pittsburgh, PA 15240, USA
REFERENCE 3 (bases 1 to 4086)
AUTHORS Frye,R.A.
TITLE Direct Submission
JOURNAL Submitted (14-APR-2000) Pathology, VA Med. Cent. (132L), Univ. of
Pittsburgh, University Drive C, Pittsburgh, PA 15240, USA
REMARK Sequence update by submitter
COMMENT On Apr 14, 2000 this sequence version replaced AF083106.1.
FEATURES Location/Qualifiers
source 1..4086
/db_xref="H-InvDB:HIT000066539"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="testis"
gene 1..4086
/gene="SIRT1"
CDS 54..2297
/gene="SIRT1"
/note="yeast silent information regulator 2 (Sir2p)
homolog"
/codon_start=1
/product="sirtuin type 1"
/protein_id="AAD40849.2"
/translation="MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGL
ERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGD
NGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSC
ESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDM
TLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSR
DGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSD
KEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVV
PRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVAL
IPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLS
EITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDL
DVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCW
PNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQS
PSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDM
NYPSNKS"
misc_feature 750..767
/gene="SIRT1"
/note="encodes putative nuclear localization signal"
BASE COUNT 1225 a 732 c 925 g 1204 t
ORIGIN
1 gtcgagcggg agcagaggag gcgagggagg agggccagag aggcagttgg aagatggcgg
61 acgaggcggc cctcgccctt cagcccggcg gctccccctc ggcggcgggg gccgacaggg
121 aggccgcgtc gtcccccgcc ggggagccgc tccgcaagag gccgcggaga gatggtcccg
181 gcctcgagcg gagcccgggc gagcccggtg gggcggcccc agagcgtgag gtgccggcgg
241 cggccagggg ctgcccgggt gcggcggcgg cggcgctgtg gcgggaggcg gaggcagagg
301 cggcggcggc aggcggggag caagaggccc aggcgactgc ggcggctggg gaaggagaca
361 atgggccggg cctgcagggc ccatctcggg agccaccgct ggccgacaac ttgtacgacg
421 aagacgacga cgacgagggc gaggaggagg aagaggcggc ggcggcggcg attgggtacc
481 gagataacct tctgttcggt gatgaaatta tcactaatgg ttttcattcc tgtgaaagtg
541 atgaggagga tagagcctca catgcaagct ctagtgactg gactccaagg ccacggatag
601 gtccatatac ttttgttcag caacatctta tgattggcac agatcctcga acaattctta
661 aagatttatt gccggaaaca atacctccac ctgagttgga tgatatgaca ctgtggcaga
721 ttgttattaa tatcctttca gaaccaccaa aaaggaaaaa aagaaaagat attaatacaa
781 ttgaagatgc tgtgaaatta ctgcaagagt gcaaaaaaat tatagttcta actggagctg
841 gggtgtctgt ttcatgtgga atacctgact tcaggtcaag ggatggtatt tatgctcgcc
901 ttgctgtaga cttcccagat cttccagatc ctcaagcgat gtttgatatt gaatatttca
961 gaaaagatcc aagaccattc ttcaagtttg caaaggaaat atatcctgga caattccagc
1021 catctctctg tcacaaattc atagccttgt cagataagga aggaaaacta cttcgcaact
1081 atacccagaa catagacacg ctggaacagg ttgcgggaat ccaaaggata attcagtgtc
1141 atggttcctt tgcaacagca tcttgcctga tttgtaaata caaagttgac tgtgaagctg
1201 tacgaggaga tatttttaat caggtagttc ctcgatgtcc taggtgccca gctgatgaac
1261 cgcttgctat catgaaacca gagattgtgt tttttggtga aaatttacca gaacagtttc
1321 atagagccat gaagtatgac aaagatgaag ttgacctcct cattgttatt gggtcttccc
1381 tcaaagtaag accagtagca ctaattccaa gttccatacc ccatgaagtg cctcagatat
1441 taattaatag agaacctttg cctcatctgc attttgatgt agagcttctt ggagactgtg
1501 atgtcataat taatgaattg tgtcataggt taggtggtga atatgccaaa ctttgctgta
1561 accctgtaaa gctttcagaa attactgaaa aacctccacg aacacaaaaa gaattggctt
1621 atttgtcaga gttgccaccc acacctcttc atgtttcaga agactcaagt tcaccagaaa
1681 gaacttcacc accagattct tcagtgattg tcacactttt agaccaagca gctaagagta
1741 atgatgattt agatgtgtct gaatcaaaag gttgtatgga agaaaaacca caggaagtac
1801 aaacttctag gaatgttgaa agtattgctg aacagatgga aaatccggat ttgaagaatg
1861 ttggttctag tactggggag aaaaatgaaa gaacttcagt ggctggaaca gtgagaaaat
1921 gctggcctaa tagagtggca aaggagcaga ttagtaggcg gcttgatggt aatcagtatc
1981 tgtttttgcc accaaatcgt tacattttcc atggcgctga ggtatattca gactctgaag
2041 atgacgtctt atcctctagt tcttgtggca gtaacagtga tagtgggaca tgccagagtc
2101 caagtttaga agaacccatg gaggatgaaa gtgaaattga agaattctac aatggcttag
2161 aagatgagcc tgatgttcca gagagagctg gaggagctgg atttgggact gatggagatg
2221 atcaagaggc aattaatgaa gctatatctg tgaaacagga agtaacagac atgaactatc
2281 catcaaacaa atcatagtgt aataattgtg caggtacagg aattgttcca ccagcattag
2341 gaactttagc atgtcaaaat gaatgtttac ttgtgaactc gatagagcaa ggaaaccaga
2401 aaggtgtaat atttataggt tggtaaaata gattgttttt catggataat ttttaacttc
2461 attatttctg tacttgtaca aactcaacac taactttttt ttttttaaaa aaaaaaaggt
2521 actaagtatc ttcaatcagc tgttgggtca agactaactt tcttttaaag gttcatttgt
2581 atgataaatt catatgtgta tatataattt tttttgtttt gtctagtgag tttcaacatt
2641 tttaaagttt tcaaaaagcc atcggaatgt taaattaatg taaagggaca gctaatctag
2701 accaaagaat ggtattttca cttttctttg taacattgaa tggtttgaag tactcaaaat
2761 ctgttacgct aaacttttga ttctttaaca caattatttt taaacactgg cattttccaa
2821 aactgtggca gctaactttt taaaatctca aatgacatgc agtgtgagta gaaggaagtc
2881 aacaatatgt ggggagagca ctcggttgtc tttactttta aaagtaatac ttggtgctaa
2941 gaatttcagg attattgtat ttacgttcaa atgaagatgg cttttgtact tcctgtggac
3001 atgtagtaat gtctatattg gctcataaaa ctaacctgaa aaacaaataa atgctttgga
3061 aatgtttcag ttgctttaga aacattagtg cctgcctgga tccccttagt tttgaaatat
3121 ttgccattgt tgtttaaata cctatcactg tggtagagct tgcattgatc ttttccacaa
3181 gtattaaact gccaaaatgt gaatatgcaa agcctttctg aatctataat aatggtactt
3241 ctactgggga gagtgtaata ttttggactg ctgttttcca ttaatgagga gagcaacagg
3301 cccctgatta tacagttcca aagtaataag atgttaattg taattcagcc agaaagtaca
3361 tgtctcccat tgggaggatt tggtgttaaa taccaaactg ctagccctag tattatggag
3421 atgaacatga tgatgtaact tgtaatagca gaatagttaa tgaatgaaac tagttcttat
3481 aatttatctt tatttaaaag cttagcctgc cttaaaacta gagatcaact ttctcagctg
3541 caaaagcttc tagtctttca agaagttcat actttatgaa attgcacagt aagcatttat
3601 ttttcagacc atttttgaac atcactccta aattaataaa gtattcctct gttgctttag
3661 tatttattac aataaaaagg gtttgaaata tagctgttct ttatgcataa aacacccagc
3721 taggaccatt actgccagag aaaaaaatcg tattgaatgg ccatttccct acttataaga
3781 tgtctcaatc tgaatttatt tggctacact aaagaatgca gtatatttag ttttccattt
3841 gcatgatgtt tgtgtgctat agatgatatt ttaaattgaa aagtttgttt taaattattt
3901 ttacagtgaa gactgttttc agctcttttt atattgtaca tagtctttta tgtaatttac
3961 tggcatatgt tttgtagact gtttaatgac tggatatctt ccttcaactt ttgaaataca
4021 aaaccagtgt tttttacttg tacactgttt taaagtctat taaaattgtc atttgacttt
4081 tttctg
//