LOCUS       AF081279                 536 bp    DNA     linear   STS 16-OCT-2001
DEFINITION  Homo sapiens U22 host gene, partial sequence; and U25 snoRNA gene,
            complete sequence, sequence tagged site.
ACCESSION   AF081279
VERSION     AF081279.1
KEYWORDS    STS.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Frey,M.R., Wu,W., Dunn,J.M. and Matera,A.G.
  TITLE     The U22 host gene (UHG): chromosomal localization of UHG and
            distribution of U22 small nucleolar RNA
  JOURNAL   Histochem. Cell Biol. 108 (4-5), 365-370 (1997)
   PUBMED   9387929
REFERENCE   2  (bases 1 to 536)
  AUTHORS   Matera,A.G. and Wu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-1998) Genetics, Case Western Reserve Univ., 10900
            Euclid Ave, Cleveland, OH 44106-4955, USA
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13; between WI-8652 and CHLC.GATA46A12"
                     /note="for radiation hybrid and FISH mapping of UHG"
     primer_bind     1..19
                     /PCR_conditions="25ng DNA, 20mM Tris- pH8.3, 2.5mM MgCl2,
                     50mM KCl, 0.2 mM dNTPs, 0.8 uM of each primer and 0.5 U
                     Taq polymerase in 10 ul; 1min 94C denaturation; 30 cycles
                     of 94C (20 sec.), a 30 sec annealing (initial annealing
                     temp. 62C, final annealing temp 47C) and 72C extension (45
                     sec; final extension for 5 min); touchdown protocol"
     gene            92..>536
                     /gene="UHG"
                     /note="U22 host gene"
     misc_RNA        join(92..112,511..>536)
                     /gene="UHG"
     exon            92..112
                     /gene="UHG"
                     /number=1
     intron          113..510
                     /gene="UHG"
                     /number=1
     ncRNA           346..412
                     /ncRNA_class="snoRNA"
                     /gene="UHG"
                     /product="U25 snoRNA"
     primer_bind     complement(518..536)
BASE COUNT           97 a          132 c          146 g          161 t
ORIGIN      
        1 cgtatgcgcg tgcgctcgcc gtagccccgc ccccaaatcc cgtgggcccc tgccggcatg
       61 gtatttaatc gtctccccgg ggagattcgt tctcattttt ctactgctcg tggtaagtgg
      121 cttcgtggtc tttatagctg ttactctttt gtactttgtc tttttctttt attttctttt
      181 gagcgattgt gcgaacatag catagcacgc actatgcctt ctgtgttgta gctgcctggc
      241 cagggcgact ggcggataag gtcttgtgcg tggcctcgag gcttaaaagt agcagtgggg
      301 ctttgtgaag gacaaaatgg cgatggcggg ccgtgtaggt cccccttcct atgatgagga
      361 ccttttcaca gacctgtact gagctccgtg aggataaata actctgagga gatgggccct
      421 gcaagcctct tgcttagccg tctgttcaga aaatagcgtt ttcgaaatgc cctgagttga
      481 cctaatgtct tattgggctc ctgtctgcag gatttacgcg cacgttggaa ccgaag
//