LOCUS AF081279 536 bp DNA linear STS 16-OCT-2001 DEFINITION Homo sapiens U22 host gene, partial sequence; and U25 snoRNA gene, complete sequence, sequence tagged site. ACCESSION AF081279 VERSION AF081279.1 KEYWORDS STS. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 536) AUTHORS Frey,M.R., Wu,W., Dunn,J.M. and Matera,A.G. TITLE The U22 host gene (UHG): chromosomal localization of UHG and distribution of U22 small nucleolar RNA JOURNAL Histochem. Cell Biol. 108 (4-5), 365-370 (1997) PUBMED 9387929 REFERENCE 2 (bases 1 to 536) AUTHORS Matera,A.G. and Wu,W. TITLE Direct Submission JOURNAL Submitted (29-JUL-1998) Genetics, Case Western Reserve Univ., 10900 Euclid Ave, Cleveland, OH 44106-4955, USA FEATURES Location/Qualifiers source 1..536 /organism="Homo sapiens" /mol_type="genomic DNA" /db_xref="taxon:9606" /chromosome="11" /map="11q13; between WI-8652 and CHLC.GATA46A12" /note="for radiation hybrid and FISH mapping of UHG" primer_bind 1..19 /PCR_conditions="25ng DNA, 20mM Tris- pH8.3, 2.5mM MgCl2, 50mM KCl, 0.2 mM dNTPs, 0.8 uM of each primer and 0.5 U Taq polymerase in 10 ul; 1min 94C denaturation; 30 cycles of 94C (20 sec.), a 30 sec annealing (initial annealing temp. 62C, final annealing temp 47C) and 72C extension (45 sec; final extension for 5 min); touchdown protocol" gene 92..>536 /gene="UHG" /note="U22 host gene" misc_RNA join(92..112,511..>536) /gene="UHG" exon 92..112 /gene="UHG" /number=1 intron 113..510 /gene="UHG" /number=1 ncRNA 346..412 /ncRNA_class="snoRNA" /gene="UHG" /product="U25 snoRNA" primer_bind complement(518..536) BASE COUNT 97 a 132 c 146 g 161 t ORIGIN 1 cgtatgcgcg tgcgctcgcc gtagccccgc ccccaaatcc cgtgggcccc tgccggcatg 61 gtatttaatc gtctccccgg ggagattcgt tctcattttt ctactgctcg tggtaagtgg 121 cttcgtggtc tttatagctg ttactctttt gtactttgtc tttttctttt attttctttt 181 gagcgattgt gcgaacatag catagcacgc actatgcctt ctgtgttgta gctgcctggc 241 cagggcgact ggcggataag gtcttgtgcg tggcctcgag gcttaaaagt agcagtgggg 301 ctttgtgaag gacaaaatgg cgatggcggg ccgtgtaggt cccccttcct atgatgagga 361 ccttttcaca gacctgtact gagctccgtg aggataaata actctgagga gatgggccct 421 gcaagcctct tgcttagccg tctgttcaga aaatagcgtt ttcgaaatgc cctgagttga 481 cctaatgtct tattgggctc ctgtctgcag gatttacgcg cacgttggaa ccgaag //