LOCUS AF077740 1453 bp mRNA linear HUM 20-MAR-2000
DEFINITION Homo sapiens 2-amino-3-ketobutyrate-CoA ligase mRNA, nuclear gene
encoding mitochondrial protein, complete cds.
ACCESSION AF077740
VERSION AF077740.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1453)
AUTHORS Edgar,A.J. and Polak,J.M.
TITLE Sequence comparison of human and mouse 2-amino-3-ketobutyrate
coenzyme A ligases
JOURNAL (in) ? (Ed.);
JOURNAL OF PATHOLOGY VOL. 187 SUPPLEMENT: 21a;
(1999)
REFERENCE 2 (bases 1 to 1453)
AUTHORS Edgar,A.J. and Polak,J.M.
TITLE Molecular cloning of the human and murine 2-amino-3-ketobutyrate
coenzyme A ligase cDNAs
JOURNAL Eur. J. Biochem. 267 (6), 1805-1812 (2000)
PUBMED 10712613
REFERENCE 3 (bases 1 to 1453)
AUTHORS Edgar,A.J.
TITLE Direct Submission
JOURNAL Submitted (14-JUL-1998) Histochemistry, Imperial College, Du Cane
Road, London W12 0NN, United Kingdom
FEATURES Location/Qualifiers
source 1..1453
/db_xref="H-InvDB:HIT000066229"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="22"
/tissue_type="lung"
CDS 4..1263
/EC_number="2.3.1.29"
/function="catalyzes the addition of acetyl-CoA to glycine
to form 2-amino-3-oxobutanoate"
/note="KBL; similar to Escherichia coli glycine
acetyltransferase, SwissProt Accession Number P07912;
similar to Bos taurus liver mitochondria
2-Amino-3-ketobutyrate-CoA ligase, PIR Accession Number
627734; similar to genomic sequence deposited in GenBank
Accession Number Z97630"
/codon_start=1
/product="2-amino-3-ketobutyrate-CoA ligase"
/protein_id="AAC27720.1"
/translation="MWPGNAWRAALFWVPRGRRAQSALAQLRGILEGELEGIRGAGTW
KSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLSSV
RFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPEDAVLSDELNHA
SIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEIC
CLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTT
GPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKM
EAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARIRVQI
SAVHSEEDIDRCVEAFVEVGRLHGALP"
misc_feature 925..927
/note="probable pyridoxal-5-phosphate attachment site;
binding site"
BASE COUNT 294 a 434 c 455 g 270 t
ORIGIN
1 gcgatgtggc ctgggaacgc ctggcgcgcc gcactcttct gggtgccccg cggccgccgc
61 gcacagtcag cgctggccca gctgcgtggc attctggagg gggagctgga aggcatccgc
121 ggagctggca cttggaagag tgagcgggtc atcacgtccc gtcaggggcc gcacatccgc
181 gtggacggcg tctccggagg aatcctcaac ttctgtgcca acaactacct gggcctgagc
241 agccaccctg aggtgattca ggcaggtctg caggctctgg aggagtttgg agctggcctt
301 agctcggtcc gcttcatctg tggaacccag agcatccaca agaatctaga agcaaaaata
361 gcccgcttcc accagcggga ggatgccatc ctctatccca gctgttatga cgccaacgcc
421 ggcctctttg aggccctgct gaccccagag gacgcagtcc tgtcggacga gctgaaccat
481 gcctccatca tcgacggcat ccggctgtgc aaggcccaca agtaccgcta tcgccacctg
541 gacatggccg acctagaagc caagctgcag gaggcccaga agcatcggct gcgcctggtg
601 gccaccgatg gggccttttc catggatggc gacatcgcac ccctgcagga gatctgctgc
661 ctcgcctcta gatatggtgc cctggtcttc atggatgaat gccatgccac tggtttcctg
721 gggcccacag gacggggcac agatgagctg ctgggtgtga tggaccaggt caccatcatc
781 aactccaccc tggggaaggc cctgggtgga gcatcagggg gctacacgac agggcctggg
841 cccctggtgt cactgctgcg gcagcgcgcc cggccatacc tcttctccaa cagtctgcca
901 cctgctgtcg ttggctgcgc ctccaaggcc ctagatctgc tgatggggag taacaccatt
961 gtccagtcta tggctgccaa gacccagagg ttccgtagta agatggaagc tgctggcttc
1021 actatctcag gagccagtca ccccatctgc cctgtgatgc tgggtgatgc ccggctggcc
1081 tctcgcatgg cggatgacat gctgaagaga ggcatctttg tcatcgggtt cagctacccc
1141 gtggtcccca agggcaaggc ccggatccgg gtacagatct cagcagtgca tagcgaggaa
1201 gacattgacc gctgcgtgga ggccttcgtg gaagtggggc gactgcacgg ggcactgccc
1261 tgagctctgg gtaaggacga gaagagccaa ggtccgcctg ctgccacagg gtcaaaggag
1321 gttttcgatc agcccagacc agaggctctg agccctgaac caaagtccca gagctgggct
1381 gggacgtgac ctgtgctgag ggctgtgaga atgtgaaaca acagtgtgaa aattggctgt
1441 gaaaaaaaaa aaa
//