LOCUS AF071076 6705 bp mRNA linear HUM 31-MAR-1999
DEFINITION Homo sapiens cell-line HeLa Nup98-Nup96 precursor, mRNA, complete
cds.
ACCESSION AF071076
VERSION AF071076.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6705)
AUTHORS Fontoura,B.M., Blobel,G. and Matunis,M.J.
TITLE A conserved biogenesis pathway for nucleoporins: proteolytic
processing of a 186-kilodalton precursor generates Nup98 and the
novel nucleoporin, Nup96
JOURNAL J. Cell Biol. 144 (6), 1097-1112 (1999)
PUBMED 10087256
REFERENCE 2 (bases 1 to 6705)
AUTHORS Fontoura,B.M.A., Blobel,G. and Matunis,M.J.
TITLE Direct Submission
JOURNAL Submitted (08-JUN-1998) Cell Biology, The Rockefeller University,
1230 York Avenue, New York, NY 10021, USA
COMMENT Radu,A. Cell 81, 215-222, 1995.
FEATURES Location/Qualifiers
source 1..6705
/db_xref="H-InvDB:HIT000065835"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="HeLa"
CDS 125..5263
/note="nucleoporin"
/codon_start=1
/product="Nup98-Nup96 precursor"
/protein_id="AAD22395.1"
/translation="MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGS
SNNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLF
SSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGT
TIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQ
VGAGTTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQ
QNQQTTSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTA
TNTSTGTAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFG
FGTNTSGNSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGA
PGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSPLFRNPMSD
PKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDE
PSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFSFLSKPV
DENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMRAALRN
GLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKITNVF
GECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVGEGL
NRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSW
VFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPPQ
SQSPEVEQLGRVVELDSDMVDITQEPVLDTMLEESMPEDQEPVSASTHIASSLGINPH
VLQIMKASLLTDEEDVDMALDQRFSRLPSKADTSQEICSPRLPISASHSSKTRSLVGG
LLQSKFTSGAFLSPSVSVQECRTPRAASLMNIPSTSSWSVPPPLTSVFTMPSPAPEVP
LKTVGTRRQLGLVPREKSVTYGKGKLLMDMALFMGRSFRVGWGPNWTLANSGEQLNGS
HELENHQIADSMEFGFLPNPVAVKPLTESPFKVHLEKLSLRQRKPDEDMKLYQTPLEL
KLKHSTVHVDELCPLIVPNLGVAVIHDYADWVKEASGDLPEAQIVKHWSLTWTLCEAL
WAHLKELDSQLNEPREYIQILERRRAFSRWLSCTATPQIEEEVSLTQKNSPVEAVFSY
LTGKRISEACSLAQQSGDHRLALLLSQFVGSQSVRELLTMQLVDWHQLQADSFIQDER
LRIFALLAGKPVWQLSEKKQINVCSQLDWKRSLAIHLWYLLPPTASISRALSMYEEAF
QNTSDSDRYACSPLPSYLEGSGCVIAEEQNSQTPLRDVCFHLLKLYSDRHYDLNQLLE
PRSITADPLDYRLSWHLWEVLRDLKYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVL
LHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHME
SDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQD
WETSGLVYLDYIRVIEMLRHIQQVEHFNDSNIVQVMTWSSYTSK"
BASE COUNT 1818 a 1620 c 1540 g 1723 t
ORIGIN
1 gcggcggcga cggtttcgtg ggggccgcgc gctgctctgt gagcggcggg tggcagcagg
61 ggactcctga cacttcccct tccccaccga accgcgcttt ctgaaacaaa gactcatttt
121 gaagatgttt aacaaatcat ttggaacacc ctttgggggt ggcacaggtg gctttggcac
181 aacttcaaca tttggacaga atactggctt tggcactact agtggagggg catttggaac
241 atctgcattt ggttctagca acaatactgg aggcctcttt ggaaattcac agactaaacc
301 aggaggattg tttggaacca gttcatttag ccagccagct acctccacaa gcactggctt
361 tgggtttggt acgtcaacag gaacagcaaa taccttgttt ggaactgcaa gcacagggac
421 cagtctcttc tcatcccaaa acaatgcctt tgcacaaaat aaaccaactg gctttggcaa
481 ttttggaacc agtactagca gtggaggact ctttggaacc acaaatacca cctctaatcc
541 ttttggcagc acatctggct ccctctttgg gccaagtagt tttacagctg ctcctactgg
601 gactactatt aaatttaacc ctccaactgg tacagatact atggtcaaag ctggagttag
661 cactaacata agtaccaagc accagtgtat tactgctatg aaagaatatg aaagcaagtc
721 actagaggaa cttcgtttag aggattatca ggctaacagg aagggcccac agaaccaggt
781 gggagcaggt accacaactg gcttgtttgg gtcttctcca gccacttcca gcgcaacagg
841 actcttcagc tcctccacca ctaattcagg ctttgcatat ggtcagaaca aaactgcctt
901 tggaactagt acaactggat ttggaacaaa tccaggtggt ctctttggcc aacagaatca
961 gcagactacc agcctcttca gcaaaccatt tggccaggct acaaccaccc agaacactgg
1021 cttttccttt ggtaatacca gcaccatagg acagccaagc accaacacca tgggattatt
1081 tggagtaacc caagcctcac agcctggagg tctttttggg acagctacaa acaccagcac
1141 tgggacagca tttggaacag gaacaggtct ctttgggcag accaatactg gatttggtgc
1201 tgttggttcg accctgtttg gcaataacaa gcttactaca tttggaagca gcacaaccag
1261 tgcaccttca tttggtacaa ccagtggcgg gctctttggt tttggcacaa ataccagtgg
1321 gaatagtatt tttggaagta aaccagcacc tgggactctt ggaactgggc ttggtgcagg
1381 atttggaaca gctcttggtg ctggacaggc atctttgttt gggaacaacc aacctaagat
1441 tggagggcct cttggtacag gagcctttgg ggcccctgga tttaatacta cgacagccac
1501 tttgggcttt ggagcccccc aggccccagt agctttgaca gatccaaatg cttctgctgc
1561 ccagcaggct gttctccagc agcacatcaa tagtctaaca tactcacctt ttggagactc
1621 tcctctcttc cggaatccga tgtcagaccc taagaagaag gaagagagat tgaaaccaac
1681 aaatccagca gcccagaagg ctcttactac acctactcat tataaactga caccccgccc
1741 tgccactaga gtccggccaa aggctttaca aacaacaggc acagccaagt cacatctctt
1801 tgatgggctg gatgacgatg aaccatccct agccaatgga gcattcatgc ccaagaagag
1861 cattaagaag ttggttttga agaaccttaa taatagcaat ctcttttctc ctgttaatcg
1921 tgattcagaa aatctagctt caccatctga atatccagaa aatggagaga gatttagttt
1981 cctaagcaaa cctgttgatg agaatcacca gcaggatgga gatgaagatt cccttgtttc
2041 acatttttat actaacccta ttgccaaacc tattcctcaa accccagaaa gtgctggaaa
2101 taaacacagc aacagcaaca gtgtggatga taccattgtt gcattaaaca tgcgtgctgc
2161 tttgcgaaat gggctggaag gaagcagtga agaaacgtct tttcatgatg agtcacttca
2221 ggatgaccga gaagaaatag aaaataattc ttaccatatg cacccagcag gtattattct
2281 cactaaggtt ggttactata ctattccatc tatggatgac cttgctaaaa ttaccaatgt
2341 ttttggagag tgcattgtct ctgatttcac tattggtcgg aaaggttatg gttcaatcta
2401 ttttgaagga gatgtgaatt tgacaaatct aaatttggat gatattgtgc atatccggag
2461 gaaagaagta gttgtctact tagatgataa ccaaaaacca cctgtgggtg aagggctaaa
2521 taggaaggct gaagttacat tggatggagt ttggccaaca gataaaacat ctcgttgttt
2581 aataaagagc ccagatcgcc ttgctgatat caactatgaa ggaagattgg aagcagtttc
2641 aaggaaacag ggagctcaat tcaaagaata ccggcctgaa actggttctt gggtgtttaa
2701 ggtctcccat ttttctaagt atggccttca ggattctgat gaagaggagg aggagcatcc
2761 gtctaaaact agtacaaaga agttgaagac tgctcctttg cctcctgcaa gccagactac
2821 gcccttgcag atggctctta atggcaaacc tgcacctcca cctcagagcc agagcccaga
2881 ggtggagcag ttagggaggg ttgtggaact ggacagtgac atggtagata tcacccagga
2941 gccagttttg gataccatgt tagaagagag catgcctgag gatcaggaac ctgtgtctgc
3001 ctcaacacat attgcatctt cactgggaat taatccacat gtcttacaga tcatgaaagc
3061 atcattgctt actgatgaag aagatgtaga tatggcactg gatcaacgct tcagtcgcct
3121 gccttccaaa gcagatactt ctcaagaaat ctgttctccc agactcccca tttcagcatc
3181 ccactcgtcg aaaactcgtt cactagttgg tgggttacta caatcaaaat ttacaagtgg
3241 agcttttctt tcaccaagtg tctctgtgca agaatgtcgt actcccagag cagcatcttt
3301 aatgaatatc ccatccacat cctcttggtc tgtccctcca cccctgactt ctgtgttcac
3361 aatgcccagc ccagcccctg aggttccgtt gaaaacagtg ggtacacgta ggcaactagg
3421 cctagtccct cgtgaaaagt ctgtcaccta tggcaaggga aaactcttga tggacatggc
3481 cctattcatg ggacgctcat ttcgtgttgg ttggggcccc aactggactc ttgctaatag
3541 tggagaacag ctgaatggct ctcatgaact agaaaatcat cagattgccg attccatgga
3601 gtttggattc ctgcccaatc cagtagctgt taaaccccta actgagtccc cattcaaagt
3661 tcacttagaa aaactcagtt tgagacaaag aaagccagat gaagacatga aattatatca
3721 gacacctctg gagctcaaat taaaacacag cactgtccat gtggatgaac tgtgtcctct
3781 cattgtcccc aatctgggag ttgctgtcat tcatgactat gcagattggg ttaaagaagc
3841 atcaggagac ttaccagaag cacaaattgt gaagcactgg agcctgacat ggacactatg
3901 tgaagcccta tgggcccacc tgaaggagct tgacagccag ctaaatgaac cccgtgaata
3961 cattcaaatt ctggagcgaa gaagagcttt ctcccgctgg ctatcctgta ctgccacacc
4021 tcagattgaa gaggaagtct ccttaaccca aaaaaacagc cctgtggagg ctgtattcag
4081 ctacctcaca ggcaaaagga tcagtgaggc ctgctctctg gcccagcagt caggggatca
4141 tcgtcttgct cttcttttat ctcagtttgt gggtagccag tcagtccggg agctgctcac
4201 catgcagttg gtggactggc atcagctcca agcagactcc ttcatccagg atgagagact
4261 gcgcatcttt gctctgttgg ctggaaaacc ggtgtggcag ctctcagaaa agaagcaaat
4321 aaacgtgtgc tcccagttgg attggaaacg ctccctggct atccatcttt ggtatttgct
4381 tccaccaaca gcctccattt ctagggcgct cagcatgtat gaagaagcat ttcagaatac
4441 ctctgacagt gacagatatg cctgctcccc acttccttcg tatctggagg gttctggctg
4501 tgtgatagcg gaggagcaaa actcacagac accacttcga gatgtctgct ttcaccttct
4561 aaaactctac agtgacagac attatgatct caaccagctg ctggagcctc gaagcataac
4621 agcagatcct ttggactacc gcctaagctg gcacttgtgg gaagtgctga gggatcttaa
4681 atacacccat ctatcagcgc agtgtgaagg tgtgctacag gccagttacg ctggccagct
4741 tgaaagtgag gggctctggg agtgggccat ctttgtcctc ctgcacattg acaactcagg
4801 catacgtgag aaggctgttc gagagctgct tacccggcac tgccagctgt tggagacccc
4861 tgaatcttgg gctaaagaga ctttccttac ccagaagctc cgtgtacctg ccaaatggat
4921 ccacgaggcc aaagctgtgc gagcacacat ggaatctgac aagcacttag aggccctttg
4981 cttatttaag gctgagcact ggaaccgctg ccacaagctc atcatccgac acttagcttc
5041 tgatgccatc attaatgaga actatgacta cctgaagggg ttcttggaag acctggcacc
5101 tccagagcgc agcagcctaa ttcaggattg ggaaacatct gggcttgttt acctggacta
5161 tattagagtc attgaaatgc tccgccatat acagcaggtg gagcatttca atgactctaa
5221 tatagtccag gtaatgacct ggagcagtta cacatcaaag tgacttcact gtgcagtcgg
5281 atagagcaga ttcagtgtta cagtgctaaa gatcgcctgg ctcagtcaga catggccaaa
5341 cgtgtagcca acctgctgcg cgtggtgctg agtctgcatc atcctcctga tagaacctcc
5401 gactcaacac cagaccctca gcgagtccct ttgcgcctct tggctcccca cattggccgg
5461 cttcccatgc ctgaggacta tgccatggac gaactgcgca gccttaccca gtcctatctg
5521 cgagaactgg ctgttgggag cctgtgagcc ccaggcactt tgcatcacag tcacatgccc
5581 attcacacca cacagaggtt ccctgccttg tttggattgg cactgtttgc cattctctgg
5641 gttggctgtg gcatctaccc tcccgtcccc tgctgccaga agcagcatcc tccacttgtt
5701 cagggctttt cttaatactg aacgtagcat aagggcttct ggaacccaga agaggagaca
5761 gtttaccatc ctcaagatca ttcagtgttt ttcctttaaa aaaatggtca ataaagctcc
5821 tttggcagaa tcccccaaag aaccagggta ttctttttcc atccttagcc attntggatc
5881 ttgtgacctt ccatgccaac cagtttcctt attcttacca tggccgtttt atattaggac
5941 tccttaggag gagtgagaca ggtgataatg gatccttaac agatgaacta tccacagaag
6001 gaagagggat ccgtctctta agtaattggt tagttaacac tgaattttgg aggcaaagga
6061 gggttggcct gagttaggaa acaaaatggg atctttctga cacacttagg gcagaagtga
6121 atgcctgtca cggagggatt gatcttcagg gctgtttttg ttcctgcctt tagagttcca
6181 tgaacaccat acctttgnta ntactatgtg caggaaccct tggtcacatg tgacatgtct
6241 gtgggaagct cccagagttt ggtttggtcc ntggttttca gtcttgctga gactctgtct
6301 ggatttgcct gcagagtttg gataaaaaat aacaggttgg taaccttcca agttcatccc
6361 atgttagctc caaagcattt cccaccctcc atctacccct tccagaaggc aaaacaaacc
6421 atgactgagg caggcatgga gttgggcgtt aggggcaggc agagggcctt tgctacactg
6481 ctgacagcta tagggagccc caggtaatgg catgagatag ctggtgttag ggctatctca
6541 ggcaatatgg ccagcacctg ggtctttatg catgaagata atgtaaaggt ttttattaaa
6601 aaatatatat atgtataaat aaatgatcta gatattttcc tctttttctg aagctacttt
6661 cttaaaaaat aaataaaatg tttatagcat tcctattaaa aaaaa
//