LOCUS AF069602 5770 bp mRNA linear HUM 03-MAR-1999
DEFINITION Homo sapiens myosin light chain kinase isoform 3A (MLCK) mRNA,
complete cds.
ACCESSION AF069602
VERSION AF069602.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5770)
AUTHORS Lazar,V.L. and Garcia,J.G.N.
TITLE A Single Human MLCK Gene Transcribes Multiple Isoforms
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5770)
AUTHORS Lazar,V.L. and Garcia,J.G.N.
TITLE Direct Submission
JOURNAL Submitted (03-JUN-1998) Medicine, Indiana University, 1481 W. 10th
Street, 111P, Indianapolis, IN 46202, USA
FEATURES Location/Qualifiers
source 1..5770
/db_xref="H-InvDB:HIT000065661"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3qcen-q21"
/cell_line="HUVEC"
/cell_type="endothelial"
/tissue_type="umbilical vein"
gene 1..5770
/gene="MLCK"
CDS 120..5708
/gene="MLCK"
/codon_start=1
/product="myosin light chain kinase isoform 3A"
/protein_id="AAD15922.1"
/translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECP
PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEVSGIPKPEVAWFLE
GTPVRRQEGSIEVYEDAGSHYLCLLKARTRDSGTYSCTASNAQGQVSCSWTLQVERLA
VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVA
ELHIQDALPEDHGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPI
FLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLWI
QEVFPEDTGTYTCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLI
SCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKN
RVGECSCQVSLMLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPAR
GQGWLEEEDGEDVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDD
LKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVP
PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAK
PAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNE
KRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIIL
SQEGSLCSVSIEKALLEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSR
RPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVT
GTQPITCTWMKFRKQIQDSEHIKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQ
AQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTW
KELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKMKWR
CQTDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKF
FKAYSAKEKENIRQEITIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIID
EDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLAR
RLENAGSLKVLFGTPEFVAPEVINYEPIDATDMWSIGVICYILNRLDCTQCLQHPWLM
KDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPT
SPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYP
DPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCT
AEHIVETMEEGEGEGEEEEE"
BASE COUNT 1497 a 1547 c 1671 g 1055 t
ORIGIN
1 ccggctgcct ctgctgcagt tcagagcaac ttcaggagct tcccagccga gagcttcagg
61 acgcctttcc tgtcccactg gcccagttgc cacaacaaac aacagagaag acggtgacca
121 tgggggatgt gaagctggtt gcctcgtcac acatttccaa aacctccctc agtgtggatc
181 cctcaagagt tgactccatg cccctgacag aggcccctgc tttcattttg ccccctcgga
241 acctctgcat caaagaagga gccaccgcca agttcgaagg gcgggtccgg ggttacccag
301 agccccaggt gacatggcac agaaacgggc aacccatcac cagcgggggc cgcttcctgc
361 tggattgcgg catccggggg actttcagcc ttgtgattca tgctgtccat gaggaggaca
421 ggggaaagta tacctgtgaa gccaccaatg gcagtggtgc tcgccaggtg acagtggagt
481 tgacagtaga aggaagtttt gcgaagcagc ttggtcagcc tgttgtttcc aaaaccttag
541 gggatagatt ttcagcttca gcagtggaga cccgtcctag catctggggg gagtgcccac
601 caaagtttgc taccaagctg ggccgagttg tggtcaaaga aggacagatg ggacgattct
661 cctgcaagat cactggccgg ccccaaccgc aggtcacctg gctcaaggga aatgttccac
721 tgcagccgag tgcccgtgtg tctgtgtctg agaagaacgg catgcaggtt ctggaaatcc
781 atggagtcaa ccaagatgac gtgggagtgt acacgtgcct ggtggtgaac gggtcgggga
841 aggcctcgat gtcagctgaa ctttccatcc aaggtttgga cagtgccaat aggtcatttg
901 tgagagaaac aaaagccacc aattcagatg tcaggaaaga ggtgaccaat gtaatctcaa
961 aggagtcgaa gctggacagt ctggaggctg cagccaaaag caagaactgc tccagccccc
1021 agagaggtgg ctccccaccc tgggctgcaa acagccagcc tcagccccca agggagtcca
1081 agctggagtc atgcaaggac tcgcccagaa cggccccgca gaccccggtc cttcagaaga
1141 cttccagctc catcaccctg caggccgcaa gagttcagcc ggaaccaaga gcaccaggcc
1201 tgggggtcct atcaccttct ggagaagaga ggaagaggcc agctcctccc cgtccagcca
1261 ccttccccac caggcagcct ggcctgggga gccaagatgt tgtgagcaag gctgctaaca
1321 ggagaatccc catggagggc cagagggatt cagcattccc caaatttgag agcaagcccc
1381 aaagccagga ggtcaaggaa aatcaaactg tcaagttcag atgtgaagtt tccgggattc
1441 caaagcctga agtggcctgg ttcctggaag gcacccccgt gaggagacag gaaggcagca
1501 ttgaggttta tgaagatgct ggctcccatt acctctgcct gctgaaagcc cggaccaggg
1561 acagtgggac atacagctgc actgcttcca acgcccaagg ccaggtgtcc tgtagctgga
1621 ccctccaagt ggaaaggctt gccgtgatgg aggtggcccc ctccttctcc agtgtcctga
1681 aggactgcgc tgttattgag ggccaggatt ttgtgctgca gtgctccgta cgggggaccc
1741 cagtgccccg gatcacttgg ctgctgaatg ggcagcccat ccagtacgct cgctccacct
1801 gcgaggccgg cgtggctgag ctccacatcc aggatgccct gccggaggac catggcacct
1861 acacctgcct agctgagaat gccttggggc aggtgtcctg cagcgcctgg gtcaccgtcc
1921 atgaaaagaa gagtagcagg aagagtgagt accttctgcc tgtggctccc agcaagccca
1981 ctgcacccat cttcctgcag ggcctctctg atctcaaagt catggatgga agccaggtca
2041 ctatgactgt ccaagtgtca gggaatccac cccctgaagt catctggctg cacaatggga
2101 atgagatcca agagtcagag gacttccact ttgaacagag aggaactcag cacagccttt
2161 ggatccagga agtgttcccg gaggacacgg gcacgtacac ctgcgaggcc tggaacagcg
2221 ctggagaggt ccgcacccag gccgtgctca cggtacaaga gcctcacgat ggcacccagc
2281 cctggttcat cagtaagcct cgctcagtga cagcctccct gggccagagt gtcctcatct
2341 cctgcgccat agctggtgac ccctttccta ccgtgcactg gctcagagat ggcaaagccc
2401 tctgcaaaga cactggccac ttcgaggtgc ttcagaatga ggacgtgttc accctggttc
2461 taaagaaggt gcagccctgg catgccggcc agtatgagat cctgctcaag aaccgggttg
2521 gcgaatgcag ttgccaggtg tcactgatgc tacagaacag ctctgccaga gcccttccac
2581 gggggaggga gcctgccagc tgcgaggacc tctgtggtgg aggagttggt gctgatggtg
2641 gtggtagtga ccgctatggg tccctgaggc ctggctggcc agcaagaggg cagggttggc
2701 tagaggagga agacggcgag gacgtgcgag gggtgctgaa gaggcgcgtg gagacgaggc
2761 agcacactga ggaggcgatc cgccagcagg aggtggagca gctggacttc cgcgacctcc
2821 tgggcaagaa ggtgagtaca aagaccctat cggaagacga cctgaaggag atcccggccg
2881 agcagatgga tttccgtgcc aacctgcagc ggcaagtgaa gccaaagact gtgtctgagg
2941 aagagaggaa ggtgcacagc ccccaacagg tcgattttcg ctccgtccta gccaagaagg
3001 ggactcccaa gacccccgtg cctgagaagg tgccaccgcc aaaacctgcc accccggatt
3061 ttcgctcagt gctgggtggc aagaagaaat taccagcaga gaatggcagc agcagtgccg
3121 agaccctgaa tgccaaggca gtggagagtt ccaagcccct gagcaatgca cagccttcag
3181 ggcccttgaa acccgtgggc aacgccaagc ctgctgagac cctgaagcca atgggcaacg
3241 ccaagcctgc cgagaccctg aagcccatgg gcaatgccaa gcctgatgag aacctgaaat
3301 ccgctagcaa agaagaactc aagaaagacg ttaagaatga tgtgaactgc aagagaggcc
3361 atgcagggac cacagataat gaaaagagat cagagagcca ggggacagcc ccagccttca
3421 agcagaagct gcaagatgtt catgtggcag agggcaagaa gctgctgctc cagtgccagg
3481 tgtcttctga ccccccagcc accatcatct ggacgctgaa tggaaagacc ctcaagacca
3541 ccaagttcat catcctctcc caggaaggct cactctgctc cgtctccatc gagaaggcac
3601 tgctcgagga cagaggctta tacaagtgtg tagccaagaa tgacgctggc caggcggagt
3661 gctcctgcca agtcaccgtg gatgatgctc cagccagtga gaacaccaag gccccagaga
3721 tgaaatcccg gaggcccaag agctctcttc ctcccgtgct aggaactgag agtgatgcga
3781 ctgtgaaaaa gaaacctgcc cccaagacac ctccgaaggc agcaatgccc cctcagatca
3841 tccagttccc tgaggaccag aaggtacgcg caggagagtc agtggagctg tttggcaaag
3901 tgacaggcac tcagcccatc acctgtacct ggatgaagtt ccgaaagcag atccaggaca
3961 gcgagcacat caaggtggag aacagcgaga atggcagcaa gctcaccatc ctggccgcgc
4021 gccaggagca ctgcggctgc tacacactgc tggtggagaa caagctgggc agcaggcagg
4081 cccaggtcaa cctcactgtc gtggataagc cagacccccc agctggcaca ccttgtgcct
4141 ctgacattcg gagctcctca ctgaccctgt cctggtatgg ctcctcatat gatgggggca
4201 gtgctgtaca gtcctacagc atcgagatct gggactcagc caacaagacg tggaaggaac
4261 tagccacatg ccgcagcacc tctttcaacg tccaggacct gctgcctgac cacgaatata
4321 agttccgtgt acgtgcaatc aacgtgtatg gaaccagtga gccaagccag gagtctgaac
4381 tcacaacggt aggagagaaa cctgaagagc cgaagatgaa gtggaggtgt cagactgatg
4441 atgagaagga gcccgaggtt gattaccgga cagtgacaat caatactgaa caaaaagtat
4501 ctgacttcta cgacattgag gagagattag gatctgggaa atttggacag gtctttcgac
4561 ttgtagaaaa gaaaactcga aaagtctggg cagggaagtt cttcaaggca tattcagcaa
4621 aagagaaaga gaatatccgg caggagatta cgatcatgaa ctgcctccac caccctaagc
4681 tggtccagtg tgtggatgcc tttgaagaaa aggccaacat cgtcatggtc ctggagatcg
4741 tgtcaggagg ggagctgttt gagcgcatca ttgacgagga ctttgagctg acggagcgtg
4801 agtgcatcaa gtacatgcgg cagatctcgg agggagtgga gtacatccac aagcagggca
4861 tcgtgcacct ggacctcaag ccggagaaca tcatgtgtgt caacaagacg ggcaccagga
4921 tcaagctcat cgactttggt ctggccagga ggctggagaa tgcggggtct ctgaaggtcc
4981 tctttggcac cccagaattt gtggctcctg aagtgatcaa ctatgagccc atcgacgcca
5041 cagacatgtg gagcatcggg gtcatctgct acatcctaaa ccgcctggac tgcacgcagt
5101 gccttcagca tccatggcta atgaaagata ccaagaacat ggaggccaag aaactctcca
5161 aggaccggat gaagaagtac atggcaagaa ggaaatggca gaaaacgggc aatgctgtga
5221 gagccattgg aagactgtcc tctatggcaa tgatctcagg gctcagtggc aggaaatcct
5281 caacagggtc accaaccagc ccgctcaatg cagaaaaact agaatctgaa gaagatgtgt
5341 cccaagcttt ccttgaggct gttgctgagg aaaagcctca tgtaaaaccc tatttctcta
5401 agaccattcg cgatttagaa gttgtggagg gaagtgctgc tagatttgac tgcaagattg
5461 aaggataccc agaccccgag gttgtctggt tcaaagatga ccagtcaatc agggagtccc
5521 gccacttcca gatagactac gatgaggacg ggaactgctc tttaattatt agtgatgttt
5581 gcggggatga cgatgccaag tacacctgca aggctgtcaa cagtcttgga gaagccacct
5641 gcacagcaga gcacattgtg gaaacgatgg aggaaggtga aggggaaggg gaagaggaag
5701 aagagtgaaa caaagccaga gaaaagcagt ttctaagtca tattaaaagg actatttctc
5761 tcaaaatcca
//