LOCUS AF069601 5719 bp mRNA linear HUM 14-MAR-2000
DEFINITION Homo sapiens myosin light chain kinase isoform 2 (MLCK) mRNA,
complete cds.
ACCESSION AF069601
VERSION AF069601.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5719)
AUTHORS Lazar,V.L. and Garcia,J.G.N.
TITLE A Single Human MLCK Gene Transcribes Multiple Isoforms
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5719)
AUTHORS Lazar,V.L. and Garcia,J.G.N.
TITLE Direct Submission
JOURNAL Submitted (03-JUN-1998) Medicine, Indiana University, 1481 W. 10th
Street, 111P, Indianapolis, IN 46202, USA
REFERENCE 3 (bases 1 to 5719)
AUTHORS Birukov,K.G. and Garcia,J.G.N.
TITLE Direct Submission
JOURNAL Submitted (14-MAR-2000) Pulmonary and Critical Care Division, Johns
Hopkins University School of Medicine, 501 Hopkins Bayview Circle,
Baltimore, MD 21224, USA
REMARK Sequence update by submitter
COMMENT On Mar 14, 2000 this sequence version replaced AF069601.1.
FEATURES Location/Qualifiers
source 1..5719
/db_xref="H-InvDB:HIT000065660"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="3"
/map="3qcen-q21"
/cell_line="HUVEC"
/cell_type="endothelial"
/tissue_type="umbilical vein"
gene 1..5719
/gene="MLCK"
CDS 120..5657
/gene="MLCK"
/codon_start=1
/product="myosin light chain kinase isoform 2"
/protein_id="AAD15921.2"
/translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECP
PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEGLAVMEVAPSFSSV
LKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVAELHIQDALPED
HGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPIFLQGLSDLKVM
DGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLWIQEVFPEDTGTY
TCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPT
VHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKNRVGECSCQVSL
MLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPARGQGWLEEEDGE
DVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDDLKEIPAEQMDF
RANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPKPATPDFRS
VLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNA
KPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTAPA
FKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSI
EKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLG
TESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK
FRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKP
DPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTWKELATCRSTSF
NVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEV
DYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKEN
IRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECI
KYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVL
FGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWD
FDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMK
KYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQA
FLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESR
HFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEE
EEE"
BASE COUNT 1492 a 1524 c 1652 g 1051 t
ORIGIN
1 ccggctgcct ctgctgcagt tcagagcaac ttcaggagct tcccagccga gagcttcagg
61 acgcctttcc tgtcccactg gcccagttgc cacaacaaac aacagagaag acggtgacca
121 tgggggatgt gaagctggtt gcctcgtcac acatttccaa aacctccctc agtgtggatc
181 cctcaagagt tgactccatg cccctgacag aggcccctgc tttcattttg ccccctcgga
241 acctctgcat caaagaagga gccaccgcca agttcgaagg gcgggtccgg ggttacccag
301 agccccaggt gacatggcac agaaacgggc aacccatcac cagcgggggc cgcttcctgc
361 tggattgcgg catccggggg actttcagcc ttgtgattca tgctgtccat gaggaggaca
421 ggggaaagta tacctgtgaa gccaccaatg gcagtggtgc tcgccaggtg acagtggagt
481 tgacagtaga aggaagtttt gcgaagcagc ttggtcagcc tgttgtttcc aaaaccttag
541 gggatagatt ttcagcttca gcagtggaga cccgtcctag catctggggg gagtgcccac
601 caaagtttgc taccaagctg ggccgagttg tggtcaaaga aggacagatg ggacgattct
661 cctgcaagat cactggccgg ccccaaccgc aggtcacctg gctcaaggga aatgttccac
721 tgcagccgag tgcccgtgtg tctgtgtctg agaagaacgg catgcaggtt ctggaaatcc
781 atggagtcaa ccaagatgac gtgggagtgt acacgtgcct ggtggtgaac gggtcgggga
841 aggcctcgat gtcagctgaa ctttccatcc aaggtttgga cagtgccaat aggtcatttg
901 tgagagaaac aaaagccacc aattcagatg tcaggaaaga ggtgaccaat gtaatctcaa
961 aggagtcgaa gctggacagt ctggaggctg cagccaaaag caagaactgc tccagccccc
1021 agagaggtgg ctccccaccc tgggctgcaa acagccagcc tcagccccca agggagtcca
1081 agctggagtc atgcaaggac tcgcccagaa cggccccgca gaccccggtc cttcagaaga
1141 cttccagctc catcaccctg caggccgcaa gagttcagcc ggaaccaaga gcaccaggcc
1201 tgggggtcct atcaccttct ggagaagaga ggaagaggcc agctcctccc cgtccagcca
1261 ccttccccac caggcagcct ggcctgggga gccaagatgt tgtgagcaag gctgctaaca
1321 ggagaatccc catggagggc cagagggatt cagcattccc caaatttgag agcaagcccc
1381 aaagccagga ggtcaaggaa aatcaaactg tcaagttcag atgtgaaggg cttgccgtga
1441 tggaggtggc cccctccttc tccagtgtcc tgaaggactg cgctgttatt gagggccagg
1501 attttgtgct gcagtgctcc gtacggggga ccccagtgcc ccggatcact tggctgctga
1561 atgggcagcc catccagtac gctcgctcca cctgcgaggc cggcgtggct gagctccaca
1621 tccaggatgc cctgccggag gaccatggca cctacacctg cctagctgag aatgccttgg
1681 ggcaggtgtc ctgcagcgcc tgggtcaccg tccatgaaaa gaagagtagc aggaagagtg
1741 agtaccttct gcctgtggct cccagcaagc ccactgcacc catcttcctg cagggcctct
1801 ctgatctcaa agtcatggat ggaagccagg tcactatgac tgtccaagtg tcagggaatc
1861 caccccctga agtcatctgg ctgcacaatg ggaatgagat ccaagagtca gaggacttcc
1921 actttgaaca gagaggaact cagcacagcc tttggatcca ggaagtgttc ccggaggaca
1981 cgggcacgta cacctgcgag gcctggaaca gcgctggaga ggtccgcacc caggccgtgc
2041 tcacggtaca agagcctcac gatggcaccc agccctggtt catcagtaag cctcgctcag
2101 tgacagcctc cctgggccag agtgtcctca tctcctgcgc catagctggt gacccctttc
2161 ctaccgtgca ctggctcaga gatggcaaag ccctctgcaa agacactggc cacttcgagg
2221 tgcttcagaa tgaggacgtg ttcaccctgg ttctaaagaa ggtgcagccc tggcatgccg
2281 gccagtatga gatcctgctc aagaaccggg ttggcgaatg cagttgccag gtgtcactga
2341 tgctacagaa cagctctgcc agagcccttc cacgggggag ggagcctgcc agctgcgagg
2401 acctctgtgg tggaggagtt ggtgctgatg gtggtggtag tgaccgctat gggtccctga
2461 ggcctggctg gccagcaaga gggcagggtt ggctagagga ggaagacggc gaggacgtgc
2521 gaggggtgct gaagaggcgc gtggagacga ggcagcacac tgaggaggcg atccgccagc
2581 aggaggtgga gcagctggac ttccgagacc tcctggggaa gaaggtgagt acaaagaccc
2641 tatcggaaga cgacctgaag gagatcccgg ccgagcagat ggatttccgt gccaacctgc
2701 agcggcaagt gaagccaaag actgtgtctg aggaagagag gaaggtgcac agcccccagc
2761 aggtcgattt tcgctctgtc ctggccaaga aggggacttc caagaccccc gtgcctgaga
2821 aggtgccacc gccaaaacct gccaccccgg attttcgctc agtgctgggt ggcaagaaga
2881 aattaccagc agagaatggc agcagcagtg ccgagaccct gaatgccaag gcagtggaga
2941 gttccaagcc cctgagcaat gcacagcctt cagggccctt gaaacccgtg ggcaacgcca
3001 agcctgctga gaccctgaag ccaatgggca acgccaagcc tgccgagacc ctgaagccca
3061 tgggcaatgc caagcctgat gagaacctga aatccgctag caaagaagaa ctcaagaaag
3121 acgttaagaa tgatgtgaac tgcaagagag gccatgcagg gaccacagat aatgaaaaga
3181 gatcagagag ccaggggaca gccccagcct tcaagcagaa gctgcaagat gttcatgtgg
3241 cagagggcaa gaagctgctg ctccagtgcc aggtgtcttc tgacccccca gccaccatca
3301 tctggacgct gaatggaaag accctcaaga ccaccaagtt catcatcctc tcccaggaag
3361 gctcactctg ctccgtctcc atcgagaagg cactgcctga ggacagaggc ttatacaagt
3421 gtgtagccaa gaatgacgct ggccaggcgg agtgctcctg ccaagtcacc gtggatgatg
3481 ctccagccag tgagaacacc aaggccccag agatgaaatc ccggaggccc aagagctctc
3541 ttcctcccgt gctaggaact gagagtgatg cgactgtgaa aaagaaacct gcccccaaga
3601 cacctccgaa ggcagcaatg ccccctcaga tcatccagtt ccctgaggac cagaaggtac
3661 gcgcaggaga gtcagtggag ctgtttggca aagtgacagg cactcagccc atcacctgta
3721 cctggatgaa gttccgaaag cagatccagg aaagcgagca catgaaggtg gagaacagcg
3781 agaatggcag caagctcacc atcctggccg cgcgccagga gcactgcggc tgctacacac
3841 tgctggtgga gaacaagctg ggcagcaggc aggcccaggt caacctcact gtcgtggata
3901 agccagaccc cccagctggc acaccttgtg cctctgacat tcggagctcc tcactgaccc
3961 tgtcctggta tggctcctca tatgatgggg gcagtgctgt acagtcctac agcatcgaga
4021 tctgggactc agccaacaag acgtggaagg aactagccac atgccgcagc acctctttca
4081 acgtccagga cctgctgcct gaccacgaat ataagttccg tgtacgtgca atcaacgtgt
4141 atggaaccag tgagccaagc caggagtctg aactcacaac ggtaggagag aaacctgaag
4201 agccgaagga tgaagtggag gtgtcagatg atgatgagaa ggagcccgag gttgattacc
4261 ggacagtgac aatcaatact gaacaaaaag tatctgactt ctacgacatt gaggagagat
4321 taggatctgg gaaatttgga caggtctttc gacttgtaga aaagaaaact cgaaaagtct
4381 gggcagggaa gttcttcaag gcatattcag caaaagagaa agagaatatc cggcaggaga
4441 ttagcatcat gaactgcctc caccacccta agctggtcca gtgtgtggat gcctttgaag
4501 aaaaggccaa catcgtcatg gtcctggaga tcgtgtcagg aggggagctg tttgagcgca
4561 tcattgacga ggactttgag ctgacggagc gtgagtgcat caagtacatg cggcagatct
4621 cggagggagt ggagtacatc cacaagcagg gcatcgtgca cctggacctc aagccggaga
4681 acatcatgtg tgtcaacaag acgggcacca ggatcaagct catcgacttt ggtctggcca
4741 ggaggctgga gaatgcgggg tctctgaagg tcctctttgg caccccagaa tttgtggctc
4801 ctgaagtgat caactatgag cccatcggct acgccacaga catgtggagc atcggggtca
4861 tctgctacat cctagtcagt ggcctttccc ccttcatggg agacaacgat aacgaaacct
4921 tggccaacgt tacctcagcc acctgggact tcgacgacga ggcattcgat gagatctccg
4981 acgatgccaa ggatttcatc agcaatctgc tgaagaaaga tatgaaaaac cgcctggact
5041 gcacgcagtg ccttcagcat ccatggctaa tgaaagatac caagaacatg gaggccaaga
5101 aactctccaa ggaccggatg aagaagtaca tggcaagaag gaaatggcag aaaacgggca
5161 atgctgtgag agccattgga agactgtcct ctatggcaat gatctcaggg ctcagtggca
5221 ggaaatcctc aacagggtca ccaaccagcc cgctcaatgc agaaaaacta gaatctgaag
5281 aagatgtgtc ccaagctttc cttgaggctg ttgctgagga aaagcctcat gtaaaaccct
5341 atttctctaa gaccattcgc gatttagaag ttgtggaggg aagtgctgct agatttgact
5401 gcaagattga aggataccca gaccccgagg ttgtctggtt caaagatgac cagtcaatca
5461 gggagtcccg ccacttccag atagactacg atgaggacgg gaactgctct ttaattatta
5521 gtgatgtttg cggggatgac gatgccaagt acacctgcaa ggctgtcaac agtcttggag
5581 aagccacctg cacagcagag ctcattgtgg aaacgatgga ggaaggtgaa ggggaagggg
5641 aagaggaaga agagtgaaac aaagccagag aaaagcagtt tctaagtcat attaaaagga
5701 ctatttctct caaaatcca
//