LOCUS       AF043333                 516 bp    mRNA    linear   HUM 25-JUL-2016
DEFINITION  Homo sapiens interleukin 10 (IL10) mRNA, partial cds.
ACCESSION   AF043333
VERSION     AF043333.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Jang,J.S. and Kim,B.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-1998) Protein Engineering, General Institute of
            Technology, Hyundai Pharm. Ind. Co., Ltd., 213 Sosa Bon 1-dong,
            Sosa-gu, Bucheon 422-231, Korea
FEATURES             Location/Qualifiers
     source          1..516
                     /db_xref="H-InvDB:HIT000063730"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /cell_type="PHA-treated peripheral blood leukocyte"
     gene            <1..516
                     /gene="IL10"
     misc_feature    1..516
                     /gene="IL10"
                     /note="Nested PCR:
                     1) first PCR :
                     forward primer (5'-aaaccacaagacagactt-3')
                     reverse primer (5'-gattttggagacctctaatttatg-3')
                     2) second PCR :
                     forward primer (5'-gtttgcgcaagcccaggccagggca-3')
                     reverse primer (5'-tcctagagtctatagagt-3')"
     CDS             <1..483
                     /gene="IL10"
                     /codon_start=1
                     /product="interleukin 10"
                     /protein_id="AAC03534.1"
                     /translation="SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLD
                     NLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLR
                     LRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
                     "
     primer_bind     <1..16
                     /note="second PCR"
                     /PCR_conditions="94C-1min, 50C-1min, 72C-3min, 30 cycles;
                     DeltaCycler II from Ericomp"
     primer_bind     complement(499..516)
                     /note="second PCR"
BASE COUNT          148 a          126 c          133 g          109 t
ORIGIN      
        1 agcccaggcc agggcaccca gtctgagaac agctgcaccc acttcccagg caacctgcct
       61 aacatgcttc gagatctccg agatgccttc agcagagtga agactttctt tcaaatgaag
      121 gatcagctgg acaacttgtt gttaaaggag tccttgctgg aggactttaa gggttacctg
      181 ggttgccaag ccttgtctga gatgatccag ttttacctgg aggaggtgat gccccaagct
      241 gagaaccaag acccagacat caaggcgcat gtgaactccc tgggggagaa cctgaagacc
      301 ctcaggctga ggctacggcg ctgtcatcga tttcttccct gtgaaaacaa gagcaaggcc
      361 gtggagcagg tgaagaatgc ctttaataag ctccaagaga aaggcatcta caaagccatg
      421 agtgagtttg acatcttcat caactacata gaagcctaca tgacaatgaa gatacgaaac
      481 tgagacatca gggtggcgac tctatagact ctagga
//