LOCUS AF043333 516 bp mRNA linear HUM 25-JUL-2016 DEFINITION Homo sapiens interleukin 10 (IL10) mRNA, partial cds. ACCESSION AF043333 VERSION AF043333.1 KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 516) AUTHORS Jang,J.S. and Kim,B.E. TITLE Direct Submission JOURNAL Submitted (15-JAN-1998) Protein Engineering, General Institute of Technology, Hyundai Pharm. Ind. Co., Ltd., 213 Sosa Bon 1-dong, Sosa-gu, Bucheon 422-231, Korea FEATURES Location/Qualifiers source 1..516 /db_xref="H-InvDB:HIT000063730" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /cell_type="PHA-treated peripheral blood leukocyte" gene <1..516 /gene="IL10" misc_feature 1..516 /gene="IL10" /note="Nested PCR: 1) first PCR : forward primer (5'-aaaccacaagacagactt-3') reverse primer (5'-gattttggagacctctaatttatg-3') 2) second PCR : forward primer (5'-gtttgcgcaagcccaggccagggca-3') reverse primer (5'-tcctagagtctatagagt-3')" CDS <1..483 /gene="IL10" /codon_start=1 /product="interleukin 10" /protein_id="AAC03534.1" /translation="SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLD NLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLR LRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN " primer_bind <1..16 /note="second PCR" /PCR_conditions="94C-1min, 50C-1min, 72C-3min, 30 cycles; DeltaCycler II from Ericomp" primer_bind complement(499..516) /note="second PCR" BASE COUNT 148 a 126 c 133 g 109 t ORIGIN 1 agcccaggcc agggcaccca gtctgagaac agctgcaccc acttcccagg caacctgcct 61 aacatgcttc gagatctccg agatgccttc agcagagtga agactttctt tcaaatgaag 121 gatcagctgg acaacttgtt gttaaaggag tccttgctgg aggactttaa gggttacctg 181 ggttgccaag ccttgtctga gatgatccag ttttacctgg aggaggtgat gccccaagct 241 gagaaccaag acccagacat caaggcgcat gtgaactccc tgggggagaa cctgaagacc 301 ctcaggctga ggctacggcg ctgtcatcga tttcttccct gtgaaaacaa gagcaaggcc 361 gtggagcagg tgaagaatgc ctttaataag ctccaagaga aaggcatcta caaagccatg 421 agtgagtttg acatcttcat caactacata gaagcctaca tgacaatgaa gatacgaaac 481 tgagacatca gggtggcgac tctatagact ctagga //