LOCUS AF039942 2952 bp mRNA linear HUM 08-JUN-2000
DEFINITION Homo sapiens HCF-binding transcription factor Zhangfei (ZF) mRNA,
complete cds.
ACCESSION AF039942
VERSION AF039942.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2952)
AUTHORS Lu,R. and Misra,V.
TITLE Zhangfei: a second cellular protein interacts with herpes simplex
virus accessory factor HCF in a manner similar to Luman and VP16
JOURNAL Nucleic Acids Res. 28 (12), 2446-2454 (2000)
PUBMED 10871379
REFERENCE 2 (bases 1 to 2952)
AUTHORS Lu,R., Yang,P. and Misra,V.
TITLE Direct Submission
JOURNAL Submitted (26-DEC-1997) Veterinary Microbiology, University of
Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N 5B4, Canada
COMMENT This cDNA clone is isolated by the same two-hybrid screening of a
HeLa cDNA library that resulted in the isolation of Luman, GenBank
accession AF009368. Northern blots indicate that the complete mRNA
(approximately 4.5KB) is longer than the sequence in this entry.
The complete mRNA may have a long 5' untranslated region. There are
stop codons at all three reading frames in front of the coding
region given in this entry, as well as a kozak-like sequence in
front of the ATG. The protein expressed from this ORF is functional
by in vitro and in vivo assays. The predicted protein sequence
also has all the structural components when aligned with other
similar proteins.
FEATURES Location/Qualifiers
source 1..2952
/db_xref="H-InvDB:HIT000063512"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="HeLa S3"
gene 1..2952
/gene="ZF"
/note="Zhangfei"
CDS 458..1276
/gene="ZF"
/note="host cell factor- or C1-binding transcription
factor; bZIP protein; similar to Luman; does not appear to
bind to consensus bZIP binding sites by itself, which may
be due to its unique basic domain"
/codon_start=1
/product="HCF-binding transcription factor Zhangfei"
/protein_id="AAD28325.1"
/translation="MEEEAIASLPGEETEDMDFLSGLELADLLDPRQPDWHLDPGLSS
PGPLSSSGGGSDSGGLWRGDDDDEAAAAEMQRFSDLLQRLLNGIGGCSSSSDSGSAEK
RRRKSPGGGGGGGSGNDNNQAATKSPRKAAAAAARLNRLKKKEYVMGLESRVRGLAAE
NQELRAENRELGKRVQALQEESRYLRAVLANETGLARLLSRLSGVGLRLTTSLFRDSP
AGDHDYALPVGKQKQDLLEEDDSAGGVCLHVDKDKVSVEFCSACARKASSSLKM"
BASE COUNT 737 a 625 c 776 g 814 t
ORIGIN
1 ggcgcggtcg agtcatcgca gggcctcacc gcttcgttct cccgtccctc cccgcgcctt
61 ggctcgacta gccaagtgag gcgggaggcg actcggacct ttccctgcat ttcgtttcgg
121 ccagtgccgg ggctacccgc cctggggcct gggatccttg gggcccgtga gccactctta
181 gcggccgggg ctaccgcggc ccgccgtggc cctcatgagg catagctgac caagctgctg
241 gcagcctcgg gcagcaactc cccaacccgc agtgagagcc cggagccggc tgcaacttgt
301 tcgctgccct ctgacctgac ccgggctgca gcgggggagg aggagacggc ggcggcgatc
361 tcccggccgc aagcagcagt ttggcgacga aggagagttg gaagccggga gggggagccg
421 cggcggcgtg gccgtgcgcg cgccctcccc cgaggagatg gaggaggagg cgatcgccag
481 cctcccgggg gaagagacgg aggatatgga ctttctgtct gggctggaac tggcggatct
541 cctggacccc aggcaaccgg actggcacct ggaccccggg cttagctcgc cggggcctct
601 ctcctcgtct ggcggaggct cggatagcgg cggcctgtgg agaggggacg atgacgatga
661 ggccgcggct gctgaaatgc agcgcttctc tgacctgctg caaaggctgt taaacggtat
721 cggaggctgc agcagcagca gtgacagtgg cagcgccgaa aagaggcgga gaaagtcccc
781 aggaggaggc ggcggtggcg gcagcggtaa cgacaacaac caggcggcga caaagagtcc
841 ccggaaggcg gcggcggccg ctgcccgcct taatcgactg aagaagaagg agtacgtgat
901 ggggctggag agtcgagtcc ggggtctggc agccgagaac caggagctgc gggccgagaa
961 tcgggagctg ggcaaacgcg tacaggcact gcaggaggag agtcgctacc tacgggcagt
1021 cttagccaac gagactggac tggctcgctt gctgagccgg ctgagcggcg tgggactgcg
1081 gctgaccacc tcgctcttca gagactcgcc cgccggtgac cacgactacg ctctgccagt
1141 gggaaagcag aagcaggacc tgctggaaga ggacgactcg gcgggaggag tctgtctcca
1201 tgtggacaag gataaggtgt cggtggagtt ctgctcggcg tgcgcccgga aggcgtcgtc
1261 ttctcttaaa atgtagggtc aagtaatctg ctctttatcc gcgtttaccc ctttcaactc
1321 ccttacacca tgtcaaactt accttagtgg gacatcttca ccggacacat ttcagaggag
1381 agaaaaaaag taatattgaa tcttaaagtg tttagctaaa agcatgaatg tgacacagta
1441 accaactcct aatgataaca tgtgactatt aaatctctct gacagtttct tttttaggtg
1501 atttccttcc tgccaggctc cgttgtaggg gttacagaac agtcgttccc gcctcacaac
1561 ctgtggatac agctgttggg gcagaagaga cgggaccagc tgctggccac atttcctgct
1621 ttattttaaa aggtagtata agaatgagga aaaagaggta atatcagggc ttctgctgtt
1681 ttttattttt aacatgttca taattaaaaa gtattttcca gcagtccaaa gatgtaagtt
1741 atcttacaca taatatgttt tattttgtta tttggttatg aaaatggaat ccttgttctt
1801 gcacaactgt aaatgttttg ttgctagata atacgatttg agacctgaat tggtctttgg
1861 tttccagtgc atcacagcat attttgtaaa atcatgtact actgcacttg agcatgaatg
1921 ggtagtagcc aaactcacaa attggagtga tgaacctgct tatacctaag ggcaggagca
1981 agcccctcac aatgcagctg catgggtttt tagtgcctac tgaattatat atatatatac
2041 atatatatat atatatataa accaaaagta gttggaaaga ttatttgaaa tgactaactt
2101 tgtgctatct ttatgaaata tgttaaatgt agcttttttg aaacagaagc cttgaattga
2161 aatttaacta atacttgaac attttgtata tatttctttg tatataattt tgtgcagtac
2221 caatgacaaa aatatggtgt cataataaaa ccaggtttgt tgatctttta gttatgggct
2281 caaagaattt attcatctct aacatgatat tggaaaataa tggatgaaaa taggaaaaat
2341 gattgttaat gctgactgtg ggtcttaaaa ggttctggaa agcagtaagt tcatttttct
2401 aaaaactata acattctgtt ggagtatttt cttccttacg tcaatacttt tcctgcatta
2461 tttgaaattg tgggctgggg agaaacagta gtcaaagctt tctgaattga gatactttga
2521 aattccaagt gtagattttt agaatgtcat tttataaatg gccgtttttg gaattacttg
2581 ataagaactt ttgaaaatgg aaggattagt atggcctatt tttaaagctg ctttgttagg
2641 ttccttatgt tttattaact gtcttttctc agtttccatt tcattttttt ttttctagtt
2701 ttggtgactt agtgattttg tcatttttta catcaacttc atggtcttgt ttttacatgg
2761 taattgcatg tacttaggat ctatctaata ggggctttaa ataaatttgg tcatatttat
2821 gtgtaagcac attttactgt aaatgtttgg gtttctgaat ttaaacagat ctgtttattt
2881 cagtatgtag taaacaatat cttaaagtgt ccgattcact acttgttaat taaaaaagtt
2941 atgattaatg tg
//