LOCUS AF025654 4546 bp mRNA linear HUM 18-DEC-1997
DEFINITION Homo sapiens mRNA capping enzyme (HCE) mRNA, complete cds.
ACCESSION AF025654
VERSION AF025654.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4546)
AUTHORS Yue,Z., Maldonado,E., Pillutla,R., Cho,H., Reinberg,D. and
Shatkin,A.J.
TITLE Mammalian capping enzyme complements mutant Saccharomyces
cerevisiae lacking mRNA guanylyltransferase and selectively binds
the elongating form of RNA polymerase II
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 94 (24), 12898-12903 (1997)
PUBMED 9371772
REFERENCE 2 (bases 1 to 4546)
AUTHORS Yue,Z., Maldonado,E., Pillutla,R.C., Cho,H., Reinberg,D. and
Shatkin,A.J.
TITLE Direct Submission
JOURNAL Submitted (19-SEP-1997) Molecular Virology, Center for Advanced
Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854,
USA
FEATURES Location/Qualifiers
source 1..4546
/db_xref="H-InvDB:HIT000062780"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="HeLa"
gene 1..4546
/gene="HCE"
CDS 289..2082
/gene="HCE"
/function="RNA guanylyltransferase; RNA 5' triphosphatase"
/codon_start=1
/product="mRNA capping enzyme"
/protein_id="AAB91559.1"
/translation="MAHNKIPPRWLNCPRRGQPVAGRFLPLKTILGPRYDSQVAEENR
FHPSMLSNYLKSLKVKMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTEN
TETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQAR
PPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDEDEDEDGKKESEPGSSASF
GKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSM
DKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLR
MHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPR
HEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKP
GRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPPNVGLLYVGGYERPFAQIKVTKELK
QYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCT
AASQGQKRKHHLDPDTELMPPPPPKRPRPLT"
BASE COUNT 1402 a 823 c 957 g 1364 t
ORIGIN
1 taccagtttg aatacatggc ccagcacctg gtgtttgcca actgcattcc tttgatccta
61 aagttcttca atcaaaacat catgtcctac atcactgcca agaacagcat ttctgtcctg
121 aatgaaagtg gcgcgccgcc cctgacgtta cccggatcgg agaggttgga attcagatta
181 cggctgcgat tcgggtgtct cggaccccgg tgtgcaccgg accacgggga ggcggctcca
241 aaggcgcggt gaacgttggt gagggagggc agctctgcgc cccaagagat ggctcacaac
301 aagatcccgc cgcggtggct gaactgtccc cggcgcggcc agccggtggc aggaagattc
361 ttacctctga agacaatatt aggaccaaga tatgatagtc aagttgctga agaaaatcgg
421 ttccatccca gcatgctctc aaattaccta aagagcctaa aggttaaaat gggcttgttg
481 gtggacctga caaatacttc aaggttctat gaccgaaatg acatagaaaa agaaggaatc
541 aaatatataa aacttcagtg taaaggacat ggtgagtgcc ctaccactga gaatactgag
601 acctttattc gtctgtgtga gcggtttaat gaaagaaatc cgcctgaact tataggtgtt
661 cattgtactc atggcttcaa tcgcactggt ttcctcatat gtgccttttt ggtggagaaa
721 atggattgga gtatcgaagc agcagttgct acttttgccc aagccagacc accaggaatc
781 tacaagggtg attatttgaa ggaacttttt cgtcggtatg gtgacataga ggaagcacca
841 cccccacctc tattgccaga ttggtgtttt gaggatgatg aagacgaaga tgaggatgag
901 gatggaaaga aggaatcaga acccgggtca agtgcttctt ttggcaaaag gagaaaagaa
961 cggttaaaac tgggcgctat tttcttggaa ggtgttactg ttaaaggtgt aactcaagta
1021 acaacacaac caaagttagg agaggtacag cagaagtgtc atcaattctg tggctgggaa
1081 gggtctggat tccctggagc acagcctgtt tccatggaca agcaaaatat taaactttta
1141 gacctgaagc catacaaagt aagctggaaa gcagatggta ctcggtacat gatgttgatt
1201 gatggcacaa atgaagtttt tatgattgat agagacaatt cagtatttca tgtttcaaat
1261 ctggaatttc catttcgtaa agatcttcgt atgcatttat caaatactct cttggatggc
1321 gagatgatta ttgacagagt aaatggacag gctgttccta gatatttgat atatgacata
1381 attaaattca attcacagcc cgttggagat tgtgatttta atgttcgtct gcagtgtata
1441 gaacgagaaa ttataagtcc tcgacacgaa aaaatgaaga ctgggctcat tgacaaaaca
1501 caggaaccat ttagcgtcag aaataagccg ttttttgaca tctgtacttc aagaaagcta
1561 cttgaaggaa attttgccaa agaagtgagc catgaaatgg atggacttat ttttcagcct
1621 actggaaaat acaaacctgg tcgatgtgat gatattttga aatggaagcc tcccagtctg
1681 aattctgtgg attttcgtct aaaaataacc agaatgggag gagaagggtt acttcctccg
1741 aatgttggcc tcctgtatgt tggaggttat gaaagaccct ttgcacaaat caaggtgaca
1801 aaagagctga aacagtatga caacaaaatt atagaatgca aatttgagaa caacagctgg
1861 gtcttcatga gacagagaac agacaaaagt tttcctaatg cctacaacac tgccatggct
1921 gtgtgtaaca gcatctcaaa ccctgtcacc aaggagatgc tgtttgagtt catcgacaga
1981 tgtactgcag cttctcaagg acagaagcga aaacatcatc tggaccctga cacggagctc
2041 atgccaccac cacctcccaa aagaccacgc cctttaacct aagacctgcc tgtgacttga
2101 gggttaagaa gaaagaggaa tgaggaaaaa acgctgttgc ccatttttgt cgccgagaaa
2161 ttgaaaaacg actgtggctt gatgttgata cacatttgaa atttttttta aaaaaaaaga
2221 attatcgtag ccagcctgta aatacttgat gcattcgttt cctcagtgct gcaatacatc
2281 atggacttaa acatcttatt tatctgataa actcagcctt accttgtgga atctgaagat
2341 tgaaatatcc aaaggtttaa acaagatgga aatcaatgaa aaagaggcct tgtttatata
2401 tgggtagctt cacatttaat ttataaagtt aacaatctgg aaactgtgag cccataaaac
2461 agtatttttt agaagttgtg ttacaactgt agcaaatttt ccttttaaaa aatccaggca
2521 atggcattaa acattcttgc tatcatttac catggatttc ctacatttct gagatccctg
2581 tattcaaaaa caaatgacag ggttgttaag ttgtactata ttaaacaact tggtctggtt
2641 tatatcattt taacaagttt tttggaaatg taaaagaaaa aagtaaagct ccatttttgt
2701 gaattgcatt gtttctaaag gaagtatttt gagtattttc atctgtttgt attgtttcga
2761 ctatgatatc agatatccag tgtgtccatt ctggcagtgg ttttgagtta cttcatggaa
2821 cttggacata caccaccagg tcctttatgt gttgttctcc tgggagactt ctttttttaa
2881 atgccaggct acttctgctt tacctcagtg gtatcagcac attgttaatt acgttaaaac
2941 acacaactca ctgtgatcta tgggagtgat atcaaataca aacgatgttg tgttccttct
3001 ctcgaacaaa acagcaaagg gaatgccagc taacttcttt gattagaagg taatgtcatg
3061 gaatagaatg ctgtcaccag aaaatgctct cccactgcta gataaatgca gaggcaaagt
3121 gtagacatct gtaggaacta aatatcaagg aaatcaagac atttattttc aaagcgagaa
3181 ttctatctac attctattct gcggatgtta tttctgttat acatctgaat tttaaaatgt
3241 ttactgatag ataatggatt taaagggcgg ctaattgatt gtctgccatt gttgataaac
3301 tgttctaatt ggtttgtgaa agaactctga gaattagcta aatggaggct agtgtattta
3361 tttttagagg tacatgtttt aagtaaatgt atttaaaaca aattttcatg gtctgaattg
3421 tatatgtaaa tgcatatctg tgtaagtata tgcaagtgta tattctttct actgatttca
3481 gtcagacacg aaatccaact gaattacccg gtagactgaa tatctgacgg gatttctata
3541 tggacatagc acagatgaac caaaaatgta tttatattca cctgagtttt aatgttttta
3601 aacaaagttt tctcctgcag tgcttttcta tatgaaatag ttgagtcaag cttgggcttt
3661 tttcttcgtt gttcccaagt catgtaatgt atagtgaatt tatccaaaac gccattgtaa
3721 cgttttttac tagagtgttt ttgcacattt gggcgctaaa gggattaaaa caactgatgc
3781 cacaatgact attgaaggag ggaaaattct caaggttcca tagtatcttt acaaaatgga
3841 ttaaaaaaaa atgcctatgc aacttcaaat agtctgatgc ctgcttcccc aggacgagtg
3901 actcttccac ggtgtgctgc tttccctccg agcctgtgga aaggcagagc ccttttgcag
3961 atacgaaagc aagcttttgc caatgatctt taatggagtt aaaatgttta tggtaattgt
4021 aacctttcaa atgagttacg tgaaaagagc atctcacttt taatacaccc agatattttc
4081 ttcaagtctt tgcgcatttt tagcaggact taattttcct aaatttattc tttcctttca
4141 attatagtga catagagtag ttggccttat aagtagatcc catttagccc ctgaacataa
4201 taccatctgc agtattataa aagtcattta gaaggtcagg gggataatct agaggcagga
4261 ttttggacat tgtgaaggaa atgtgctcct tctcagctca cttcaataac tattttctga
4321 gactgaagtt ttttagacaa gaataagaaa actttgcttt cttcagttat cacatgtgaa
4381 agcttttgct ttttgttagg aaaaggtgtg gatgactcta ctgcgtggat ggttacattt
4441 gctttccgtg aaatgctcag aaaatgtcag gacattcctt ttccaattgt gtgtcagtgc
4501 gtattgtaat aaaactactg atggaattcc ggaaaaaaaa aaaaaa
//