LOCUS AB451470 1359 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens GPRC5C mRNA for G protein-coupled receptor family C
group 5 member C isoform b, partial cds, clone: FLJ16084SAAF.
ACCESSION AB451470
VERSION AB451470.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1359)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with
spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
the ORF. This is an F-type clone that deletes the stop codon for
C-terminal tagged proteins. DNA sequences of the entire ORF and
flanking regions were validated by sequencing. Clone structure of
the ORF and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
attL sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1359
/clone="FLJ16084SAAF"
/db_xref="H-InvDB:HIT000487683"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..>1359
/codon_start=1
/gene="GPRC5C"
/product="G protein-coupled receptor family C group 5
member C isoform b"
/protein_id="BAG70284.1"
/transl_table=1
/translation="MGTQPEPGLGARMAIHKALVMCLGLPLFLFPGAWAQGHVPPGCS
QGLNPLYYNLCDRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQ
VFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKN
HGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIAN
MDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYT
YGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPEVSQVTKSSPEQSYQGDMYPTRGV
GYETILKEQKGQSMFVENKAFSMDEPVAAKRPVSPYSGYNGQLLTSVYQPTEMALMHK
VPSEGAYDIILPRATANSQVMGSANSTLRAEDMYSAQSHQAATPPKDGKNSQVFRNPY
VWD"
BASE COUNT 240 a 442 c 398 g 279 t
ORIGIN
1 atggggaccc aaccagagcc tggcctggga gccaggatgg ccatccacaa agccttggtg
61 atgtgcctgg gactgcctct cttcctgttc ccaggggcct gggcccaggg ccatgtccca
121 cccggctgca gccaaggcct caaccccctg tactacaacc tgtgtgaccg ctctggggcg
181 tggggcatcg tcctggaggc cgtggctggg gcgggcattg tcaccacgtt tgtgctcacc
241 atcatcctgg tggccagcct cccctttgtg caggacacca agaaacggag cctgctgggg
301 acccaggtat tcttccttct ggggaccctg ggcctcttct gcctcgtgtt tgcctgtgtg
361 gtgaagcccg acttctccac ctgtgcctct cggcgcttcc tctttggggt tctgttcgcc
421 atctgcttct cttgtctggc ggctcacgtc tttgccctca acttcctggc ccggaagaac
481 cacgggcccc ggggctgggt gatcttcact gtggctctgc tgctgaccct ggtagaggtc
541 atcatcaata cagagtggct gatcatcacc ctggttcggg gcagtggcga gggcggccct
601 cagggcaaca gcagcgcagg ctgggccgtg gcctccccct gtgccatcgc caacatggac
661 tttgtcatgg cactcatcta cgtcatgctg ctgctgctgg gtgccttcct gggggcctgg
721 cccgccctgt gtggccgcta caagcgctgg cgtaagcatg gggtctttgt gctcctcacc
781 acagccacct ccgttgccat atgggtggtg tggatcgtca tgtatactta cggcaacaag
841 cagcacaaca gtcccacctg ggatgacccc acgctggcca tcgccctcgc cgccaatgcc
901 tgggccttcg tcctcttcta cgtcatcccc gaggtctccc aggtgaccaa gtccagccca
961 gagcaaagct accaggggga catgtacccc acccggggcg tgggctatga gaccatcctg
1021 aaagagcaga agggtcagag catgttcgtg gagaacaagg ccttttccat ggatgagccg
1081 gttgcagcta agaggccggt gtcaccatac agcgggtaca atgggcagct gctgaccagt
1141 gtgtaccagc ccactgagat ggccctgatg cacaaagttc cgtccgaagg agcttacgac
1201 atcatcctcc cacgggccac cgccaacagc caggtgatgg gcagtgccaa ctcgaccctg
1261 cgggctgaag acatgtactc ggcccagagc caccaggcgg ccacaccgcc gaaagacggc
1321 aagaactctc aggtctttag aaacccctac gtgtgggac
//