LOCUS AB451469 1938 bp mRNA linear HUM 02-SEP-2008
DEFINITION Homo sapiens AKAP8L mRNA for A-kinase anchor protein 8-like,
partial cds, clone: FLJ10732SAAF.
ACCESSION AB451469
VERSION AB451469.1
KEYWORDS Gateway cloning system.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1938)
AUTHORS Kawamura,Y., Nomura,N. and Goshima,N.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Naoki Goshima
Biomedicinal Information Research Center (BIRC), National
Institute of Advanced Industrial Science and Technology (AIST);
2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE 2
AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
Imai,J.-I., Watanabe,S. and Nomura,N.
TITLE Human Protein Factory: an infrastructure to convert the human
transcriptome into the in vitro-expressed human proteome of
versatile utility
JOURNAL Unpublished (2008)
COMMENT This human Gateway entry clone was constructed by recombining an
attB-attached open reading frame (ORF) fragment with attP
sequences of the Gateway donor vector pDONR201. The ORF sequence
in the entry clone is flanked with attL1 sequence (100 nt) -
spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
translational initiation codon (ATG) and is also flanked with
spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
the ORF. This is an F-type clone that deletes the stop codon for
C-terminal tagged proteins. DNA sequences of the entire ORF and
flanking regions were validated by sequencing. Clone structure of
the ORF and flanking sequences is as follows:
5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
attL sequences are described in lower cases).
Clone information: http://www.HGPD.jp/
This clone was produced in the "Functional Analysis of Protein and
Research Application" project supported by the New Energy and
Industrial Technology Development Organization (NEDO), Japan
FEATURES Location/Qualifiers
source 1..1938
/clone="FLJ10732SAAF"
/db_xref="H-InvDB:HIT000487682"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="Vector: pDONR201"
/organism="Homo sapiens"
CDS 1..>1938
/codon_start=1
/gene="AKAP8L"
/product="A-kinase anchor protein 8-like"
/protein_id="BAG70283.1"
/transl_table=1
/translation="MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEG
YGYGYGYGQDNTTNYGYGMATSHSWEMPSSDTNANTSASGSASADSVLSRINQRLDMV
PHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLYRSGYDYSELDPEMEMAYEGQ
YDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQCMSGASRL
PSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKK
KRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEE
GKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCS
LCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTV
EDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLFIPMQFGIIQKHLKTMDHN
RNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQEEAEGGA
LDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQ
RQIRGIPGLDVEDDEEGGGGAP"
BASE COUNT 481 a 522 c 627 g 308 t
ORIGIN
1 atgagctaca caggctttgt ccagggatct gaaaccactt tgcagtcgac atactcggat
61 accagcgctc agcccacctg tgattatgga tatggaactt ggaactctgg gacaaataga
121 ggctacgagg gctatggcta tggctatggc tatggccagg ataacaccac caactatggg
181 tatggtatgg ccacttcaca ctcttgggaa atgcctagct ctgacacaaa tgcaaacact
241 agtgcctcgg gtagcgccag tgccgattcc gttttatcca gaattaacca gcgcttagat
301 atggtgccgc atttggagac agacatgatg caaggaggcg tgtacggctc aggtggagaa
361 aggtatgact cttatgagtc ctgcgactcg agggccgtcc tgagtgagcg cgacctgtac
421 cggtcaggct atgactacag cgagcttgac cctgagatgg aaatggccta tgagggccaa
481 tacgatgcct accgcgacca gttccgcatg cgtggcaacg acaccttcgg tcccagggca
541 cagggctggg cccgggatgc ccggagcggc cggccaatgg cctcaggcta tgggcgcatg
601 tgggaagacc ccatgggggc ccggggccag tgcatgtctg gtgcctctcg gctgccctcc
661 ctcttctccc agaacatcat ccccgagtac ggcatgttcc agggcatgcg aggtgggggc
721 gccttcccgg gcggctcccg ctttggtttc gggtttggca atggcatgaa gcagatgagg
781 cggacctgga agacctggac cacagccgac ttccgaacca agaagaagaa gagaaagcag
841 ggcggcagtc ctgatgagcc agatagcaaa gccacccgca cggactgctc ggacaacagc
901 gactcagaca atgatgaggg caccgagggg gaagccacag agggccttga aggcaccgag
961 gctgtggaga agggctccag agtggacgga gaggatgagg agggaaaaga ggatgggaga
1021 gaagaaggca aagaggatcc agagaagggg gccctaacca cccaggatga aaatggccag
1081 accaagcgca agttgcaggc aggcaagaag agtcaggaca agcagaaaaa gcggcagcga
1141 gaccgcatgg tggaaaggat ccagtttgtg tgttctctgt gcaaataccg gaccttctat
1201 gaggacgaga tggccagcca tcttgacagc aagttccaca aggaacactt taagtacgta
1261 ggcaccaagc tccctaagca gacggctgac tttctgcagg agtacgtcac taacaagacc
1321 aagaagacag aggagctccg aaaaaccgtg gaggaccttg atggcctcat ccagcaaatc
1381 tacagagacc aggatctgac ccaggaaatt gccatggagc attttgtgaa gaaggtggag
1441 gcagcccatt gtgcagcctg cgacctcttc attcccatgc agtttgggat catccagaag
1501 catctgaaga ccatggatca caaccggaac cgcaggctca tgatggagca gtccaagaag
1561 tcctccctca tggtggcccg cagtattctc aacaacaagc tcatcagcaa gaagctggag
1621 cgctacctga agggcgagaa ccctttcacc gacagccccg aggaggagaa ggagcaggag
1681 gaggctgagg gcggtgccct ggacgagggg gcgcagggcg aagcggcagg gatctcggag
1741 ggcgcagagg gcgtgccggc gcagcctccc gtgcccccag agccagcccc cggggccgtg
1801 tcgccgccac cgccgccgcc cccagaggag gaggaggagg gcgccgtgcc cttgctggga
1861 ggggcgctgc aacgccagat ccgcggcatc ccgggcctcg acgtggagga cgacgaggag
1921 ggcggcgggg gcgccccg
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