LOCUS       AB451447                 594 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens YKT6 mRNA for YKT6 v-SNARE protein, partial cds,
            clone: FLJ08184AAAF.
ACCESSION   AB451447
VERSION     AB451447.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with
            spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
            the ORF. This is an F-type clone that deletes the stop codon for
            C-terminal tagged proteins. DNA sequences of the entire ORF and
            flanking regions were validated by sequencing. Clone structure of
            the ORF and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
            gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
            attL sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..594
                     /clone="FLJ08184AAAF"
                     /db_xref="H-InvDB:HIT000487660"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..>594
                     /codon_start=1
                     /gene="YKT6"
                     /product="YKT6 v-SNARE protein"
                     /protein_id="BAG70261.1"
                     /transl_table=1
                     /translation="MKLYSLSVLYKGEAKVVLLKAAYDVSSLSFFQRSSVQEFMTFTS
                     QLIVERSSKGTRASVKEQDYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDEFS
                     KQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESL
                     LERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM"
BASE COUNT          165 a          153 c          143 g          133 t
ORIGIN      
        1 atgaagctgt acagcctcag cgtcctctac aaaggcgagg ccaaggtggt gctgctcaaa
       61 gccgcatacg atgtgtcttc cctcagcttt ttccagagat ccagcgttca ggaattcatg
      121 accttcacga gtcaactgat tgtggagcgc tcatcgaaag gcactagagc ttctgtcaaa
      181 gaacaagact atctgtgcca cgtctacgtc cggaatgata gtcttgcagg tgtggtcatt
      241 gctgacaatg aatacccatc ccgggtggcc tttaccttgc tggagaaggt actagatgaa
      301 ttctccaagc aagtcgacag gatagactgg ccagtaggat cccctgctac aatccattac
      361 ccagccctgg atggtcacct cagtagatac cagaacccac gagaagctga tcccatgact
      421 aaagtgcagg ccgaactaga tgagaccaaa atcattctgc acaacaccat ggagtctctg
      481 ttagagcgag gtgagaagct agatgacttg gtgtccaaat ccgaggtgct gggaacacag
      541 tctaaagcct tctataaaac tgcccggaaa caaaactcat gctgtgccat catg
//