LOCUS       AB451444                 519 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens PTP4A1 mRNA for protein tyrosine phosphatase type IVA
            protein 1, partial cds, clone: FLJ08177AAAF.
ACCESSION   AB451444
VERSION     AB451444.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with
            spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
            the ORF. This is an F-type clone that deletes the stop codon for
            C-terminal tagged proteins. DNA sequences of the entire ORF and
            flanking regions were validated by sequencing. Clone structure of
            the ORF and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
            gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
            attL sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..519
                     /clone="FLJ08177AAAF"
                     /db_xref="H-InvDB:HIT000487657"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..>519
                     /codon_start=1
                     /gene="PTP4A1"
                     /product="protein tyrosine phosphatase type IVA protein 1"
                     /protein_id="BAG70258.1"
                     /transl_table=1
                     /translation="MARMNRPAPVEATYKNMRFLITHNPTNATLNKFIEELKKYGVTT
                     IVRVCEATYDTTLVEKEGIHVLDWPFDDGAPPSNQIVDDWLSLVKIKFREEPGCCIAV
                     HCVAGLGRAPVLVALALIEGGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRF
                     KDSNGHRNNCCIQ"
BASE COUNT          162 a           98 c          116 g          143 t
ORIGIN      
        1 atggctcgaa tgaaccgccc agctcctgtg gaagccacat acaagaacat gagatttctt
       61 attacacaca atccaaccaa tgcgacctta aacaaattta tagaggaact taagaagtat
      121 ggagttacca caatagtaag agtatgtgaa gcaacttatg acactactct tgtggagaaa
      181 gaaggtatcc atgttcttga ttggcctttt gatgatggtg caccaccatc caaccagatt
      241 gttgatgact ggttaagtct tgtgaaaatt aagtttcgtg aagaacctgg ttgttgtatt
      301 gctgttcatt gcgttgcagg ccttgggaga gctccagtac ttgttgccct agcattaatt
      361 gaaggtggaa tgaaatacga agatgcagta caattcataa gacaaaagcg gcgtggagct
      421 tttaacagca agcaacttct gtatttggag aagtatcgtc ctaaaatgcg gctgcgtttc
      481 aaagattcca acggtcatag aaacaactgt tgcattcaa
//