LOCUS AB451444 519 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens PTP4A1 mRNA for protein tyrosine phosphatase type IVA protein 1, partial cds, clone: FLJ08177AAAF. ACCESSION AB451444 VERSION AB451444.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 519) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..519 /clone="FLJ08177AAAF" /db_xref="H-InvDB:HIT000487657" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>519 /codon_start=1 /gene="PTP4A1" /product="protein tyrosine phosphatase type IVA protein 1" /protein_id="BAG70258.1" /transl_table=1 /translation="MARMNRPAPVEATYKNMRFLITHNPTNATLNKFIEELKKYGVTT IVRVCEATYDTTLVEKEGIHVLDWPFDDGAPPSNQIVDDWLSLVKIKFREEPGCCIAV HCVAGLGRAPVLVALALIEGGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRF KDSNGHRNNCCIQ" BASE COUNT 162 a 98 c 116 g 143 t ORIGIN 1 atggctcgaa tgaaccgccc agctcctgtg gaagccacat acaagaacat gagatttctt 61 attacacaca atccaaccaa tgcgacctta aacaaattta tagaggaact taagaagtat 121 ggagttacca caatagtaag agtatgtgaa gcaacttatg acactactct tgtggagaaa 181 gaaggtatcc atgttcttga ttggcctttt gatgatggtg caccaccatc caaccagatt 241 gttgatgact ggttaagtct tgtgaaaatt aagtttcgtg aagaacctgg ttgttgtatt 301 gctgttcatt gcgttgcagg ccttgggaga gctccagtac ttgttgccct agcattaatt 361 gaaggtggaa tgaaatacga agatgcagta caattcataa gacaaaagcg gcgtggagct 421 tttaacagca agcaacttct gtatttggag aagtatcgtc ctaaaatgcg gctgcgtttc 481 aaagattcca acggtcatag aaacaactgt tgcattcaa //